Escherichia coli isolates from patients with inflammatory bowel
disease: ExPEC virulence- and colicin-determinants are more
frequent compared to healthy controls

J 2018

Escherichia coli isolates from patients with inflammatory bowel disease: ExPEC virulence- and colicin-determinants are more frequent compared to healthy controls

MICENKOVÁ, Lenka, Lucia FRANKOVIČOVÁ, Iva JABORNÍKOVÁ, Juraj BOSÁK, Petr DÍTĚ et. al.

Základní údaje

Originální název

Escherichia coli isolates from patients with inflammatory bowel disease: ExPEC virulence- and colicin-determinants are more frequent compared to healthy controls

Autoři

MICENKOVÁ, Lenka (703 Slovensko, domácí), Lucia FRANKOVIČOVÁ (703 Slovensko), Iva JABORNÍKOVÁ (203 Česká republika, domácí), Juraj BOSÁK (703 Slovensko, domácí), Petr DÍTĚ (203 Česká republika, domácí), Jan ŠMARDA (203 Česká republika, domácí), Martin VRBA (203 Česká republika), Alena ŠEVČÍKOVÁ (203 Česká republika), Marta KMEŤOVÁ (703 Slovensko) a David ŠMAJS (203 Česká republika, garant, domácí)

Vydání

International Journal of Medical Microbiology, JENA, Urban & Fischer Verlag, 2018, 1438-4221

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.362

Kód RIV

RIV/00216224:14110/18:00101321

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.ijmm.2018.04.008

UT WoS

000440393900002

Klíčová slova anglicky

E. coli; Crohn's disease; Ulcerative colitis; Virulence; Bacteriocin; Phylogenetic group

Štítky

14110213, 14110513, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 3. 2019 10:33, Soňa Böhmová

Anotace

V originále

A set of 178 Escherichia coli isolates taken from patients with inflammatory bowel disease (IBD) was analyzed for bacteriocin production and tested for the prevalence of 30 bacteriocin and 22 virulence factor determinants. Additionally, E. coli phylogenetic groups were also determined. Pulsed-field gel electrophoresis (PFGE) was used for exclusion of clonal character of isolates. Results were compared to data from a previously published analysis of 1283 fecal commensal E. coil isolates. The frequency of bacteriocinogenic isolates (66.9%) was significantly higher in IBD E. coli compared to fecal commensal E. coli isolates (54.2%, p < 0.01). In the group of IBD E. call isolates, a higher prevalence of determinants for group B colicins (i.e., colicins B, D, Ia, Ib, M, and 5/10) (p < 0.01), including a higher prevalence of the colicin B determinant (p < 0.01) was found. Virulence factor determinants encoding fimbriae (fin/A, 91.0%; pap, 27.5%), cytotoxic necrotizing factor (cnfl, 11.2%), aerobactin synthesis (aer, 43.3%), and the locus associated with invasivity (ial, 9.0%) were more prevalent in IBD E. coli (p < 0.05 for all five determinants). E. coli isolates from IBD mucosal biopsies were more frequently bacteriocinogenic (84.6%, p < 0.01) compared to fecal IBD isolates and fecal commensal E. coli. PFGE analysis revealed clusters specific for IBD E. coli isolates (n = 11), for fecal isolates (n = 13), and clusters containing both IBD and fecal isolates (n = 10). ExPEC (Extraintestinal Pathogenic E. coli) virulence and colicin determinants appear to be important characteristics of IBD E. coil isolates, especially the E. coli isolates obtained directly from biopsy samples.

Návaznosti

EF15_003/0000469, projekt VaV

Název: Cetocoen Plus

GA16-21649S, projekt VaV

Název: Molekulární charakterizace nových bakteriocinů identifikovaných v rodech Escherichia a Shigella

Investor: Grantová agentura ČR, Molekulární charakterizace nových bakteriocinů identifikovaných v rodech Escherichia a Shigella

LM2015051, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

ROZV/25/LF/2017, interní kód MU

Název: LF - Příspěvek na IP 2017

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, LF - Příspěvek na IP 2017, Interní rozvojové projekty

Citovat

MICENKOVÁ, Lenka, Lucia FRANKOVIČOVÁ, Iva JABORNÍKOVÁ, Juraj BOSÁK, Petr DÍTĚ, Jan ŠMARDA, Martin VRBA, Alena ŠEVČÍKOVÁ, Marta KMEŤOVÁ a David ŠMAJS. Escherichia coli isolates from patients with inflammatory bowel disease: ExPEC virulence- and colicin-determinants are more frequent compared to healthy controls. International Journal of Medical Microbiology. JENA: Urban & Fischer Verlag, 2018, roč. 308, č. 5, s. 498-504. ISSN 1438-4221. Dostupné z: https://dx.doi.org/10.1016/j.ijmm.2018.04.008.

@article{1461537,
   author = {Micenková, Lenka and Frankovičová, Lucia and Jaborníková, Iva and Bosák, Juraj and Dítě, Petr and Šmarda, Jan and Vrba, Martin and Ševčíková, Alena and Kmeťová, Marta and Šmajs, David},
   article_location = {JENA},
   article_number = {5},
   doi = {http://dx.doi.org/10.1016/j.ijmm.2018.04.008},
   keywords = {E. coli; Crohn's disease; Ulcerative colitis; Virulence; Bacteriocin; Phylogenetic group},
   language = {eng},
   issn = {1438-4221},
   journal = {International Journal of Medical Microbiology},
   title = {Escherichia coli isolates from patients with inflammatory bowel disease: ExPEC virulence- and colicin-determinants are more frequent compared to healthy controls},
   volume = {308},
   year = {2018}
}

TY  - JOUR
ID  - 1461537
AU  - Micenková, Lenka - Frankovičová, Lucia - Jaborníková, Iva - Bosák, Juraj - Dítě, Petr - Šmarda, Jan - Vrba, Martin - Ševčíková, Alena - Kmeťová, Marta - Šmajs, David
PY  - 2018
TI  - Escherichia coli isolates from patients with inflammatory bowel disease: ExPEC virulence- and colicin-determinants are more frequent compared to healthy controls
JF  - International Journal of Medical Microbiology
VL  - 308
IS  - 5
SP  - 498-504
EP  - 498-504
PB  - Urban & Fischer Verlag
SN  - 14384221
KW  - E. coli
KW  - Crohn's disease
KW  - Ulcerative colitis
KW  - Virulence
KW  - Bacteriocin
KW  - Phylogenetic group
N2  - A set of 178 Escherichia coli isolates taken from patients with inflammatory bowel disease (IBD) was analyzed for bacteriocin production and tested for the prevalence of 30 bacteriocin and 22 virulence factor determinants. Additionally, E. coli phylogenetic groups were also determined. Pulsed-field gel electrophoresis (PFGE) was used for exclusion of clonal character of isolates. Results were compared to data from a previously published analysis of 1283 fecal commensal E. coil isolates. The frequency of bacteriocinogenic isolates (66.9%) was significantly higher in IBD E. coli compared to fecal commensal E. coli isolates (54.2%, p < 0.01). In the group of IBD E. call isolates, a higher prevalence of determinants for group B colicins (i.e., colicins B, D, Ia, Ib, M, and 5/10) (p < 0.01), including a higher prevalence of the colicin B determinant (p < 0.01) was found. Virulence factor determinants encoding fimbriae (fin/A, 91.0%; pap, 27.5%), cytotoxic necrotizing factor (cnfl, 11.2%), aerobactin synthesis (aer, 43.3%), and the locus associated with invasivity (ial, 9.0%) were more prevalent in IBD E. coli (p < 0.05 for all five determinants). E. coli isolates from IBD mucosal biopsies were more frequently bacteriocinogenic (84.6%, p < 0.01) compared to fecal IBD isolates and fecal commensal E. coli. PFGE analysis revealed clusters specific for IBD E. coli isolates (n = 11), for fecal isolates (n = 13), and clusters containing both IBD and fecal isolates (n = 10). ExPEC (Extraintestinal Pathogenic E. coli) virulence and colicin determinants appear to be important characteristics of IBD E. coil isolates, especially the E. coli isolates obtained directly from biopsy samples.
ER  -

MICENKOVÁ, Lenka, Lucia FRANKOVIČOVÁ, Iva JABORNÍKOVÁ, Juraj BOSÁK, Petr DÍTĚ, Jan ŠMARDA, Martin VRBA, Alena ŠEVČÍKOVÁ, Marta KMEŤOVÁ a David ŠMAJS. Escherichia coli isolates from patients with inflammatory bowel disease: ExPEC virulence- and colicin-determinants are more frequent compared to healthy controls. \textit{International Journal of Medical Microbiology}. JENA: Urban \&{} Fischer Verlag, 2018, roč.~308, č.~5, s.~498-504. ISSN~1438-4221. Dostupné z: https://dx.doi.org/10.1016/j.ijmm.2018.04.008.

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