Study of Hemeoxygenase-1 and Biliverdin Reductase expression in
the Choroid Plexus after Subarachnoid Hemorrhage

a 2018

Study of Hemeoxygenase-1 and Biliverdin Reductase expression in the Choroid Plexus after Subarachnoid Hemorrhage

SINAI CACODCAR, Rahul Sanjiva, Peter SOLÁR, Ilona KLUSÁKOVÁ, Petr DUBOVÝ, Marek JOUKAL et. al.

Základní údaje

Originální název

Study of Hemeoxygenase-1 and Biliverdin Reductase expression in the Choroid Plexus after Subarachnoid Hemorrhage

Autoři

SINAI CACODCAR, Rahul Sanjiva (620 Portugalsko), Peter SOLÁR (703 Slovensko), Ilona KLUSÁKOVÁ (203 Česká republika), Petr DUBOVÝ (203 Česká republika) a Marek JOUKAL (203 Česká republika, garant, domácí)

Vydání

Morphology 2018, 2018

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30103 Neurosciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/18:00104361

Organizační jednotka

Lékařská fakulta

ISBN

978-80-88064-35-0

Klíčová slova česky

hemoxygenáza; biliverdinreduktáza; subarachnoidální krvácení; krevně-likvorová bariéra; plexus choroideus; HO-1; BVR; CP; SAH

Klíčová slova anglicky

Hemoxygenase-1; biliverdin reductase; subarachnoidal hemorrhage; blood-CSF barrier; plexus choroideus

Změněno: 5. 12. 2018 09:12, doc. MUDr. Marek Joukal, Ph.D.

Anotace

V originále

Subarachnoid hemorrhage (SAH) is a form of hemorrhagic stroke, associated with significant morbidity and mortality of affected patients. Complications after SAH include seizures, neurological deficits and an edematous state resulting from affected cerebrospinal fluid (CSF) circulatory dynamics. Blood clot degradation leads to activation of enzymes Hemeoxygenase-1 (HO-1) and Biliverdin reductase (BVR). The aim of our study was to describe the expression of HO-1 and BVR in the choroid plexus (CP) after SAH. Wistar rats (n=34; male) were used in our experiment. Injection of autologous arterial blood (SAH group) or artificial CSF (control group) to the cisterna magna was performed and rats were left to survive for 1, 3 and 7 days. SAH and control animals were sacrificed together with naive rats and coronal cryostat sections of the brain were prepared. Immunohistochemical detection of HO-1 and BVR enzymes in CP was performed, quantified and statistically analyzed. HO-1 was expressed in cuboidal cells (CC) and epiplexus cells (EC) in all groups of animals. After SAH, a higher statistically significant increase of HO-1 was observed in the CP compared with control and naive animals. Moreover, SAH induced increased number of HO-1+ EC following the time of survival. BVR was found in erythrocytes forming the clot on CP in animals 1 day after SAH. Subsequent survival times showed clot resolution and increased BVR expression in CC. An increased expression of HO-1 and BVR in CP was observed after SAH, suggesting possible disruption of CSF homeostasis and hydrocephalus formation.

Návaznosti

MUNI/A/0908/2017, interní kód MU

Název: Reakce plexus choroideus laboratorního potkana na subarachnoidální krvácení

Investor: Masarykova univerzita, Reakce plexus choroideus laboratorního potkana na subarachnoidální krvácení, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

SINAI CACODCAR, Rahul Sanjiva, Peter SOLÁR, Ilona KLUSÁKOVÁ, Petr DUBOVÝ a Marek JOUKAL. Study of Hemeoxygenase-1 and Biliverdin Reductase expression in the Choroid Plexus after Subarachnoid Hemorrhage. In Morphology 2018. 2018. ISBN 978-80-88064-35-0.

@proceedings{1463196,
   author = {Sinai Cacodcar, Rahul Sanjiva and Solár, Peter and Klusáková, Ilona and Dubový, Petr and Joukal, Marek},
   booktitle = {Morphology 2018},
   keywords = {Hemoxygenase-1; biliverdin reductase; subarachnoidal hemorrhage; blood-CSF barrier; plexus choroideus},
   language = {eng},
   isbn = {978-80-88064-35-0},
   title = {Study of Hemeoxygenase-1 and Biliverdin Reductase expression in the Choroid Plexus after Subarachnoid Hemorrhage},
   year = {2018}
}

TY  - CONF
ID  - 1463196
AU  - Sinai Cacodcar, Rahul Sanjiva - Solár, Peter - Klusáková, Ilona - Dubový, Petr - Joukal, Marek
PY  - 2018
TI  - Study of Hemeoxygenase-1 and Biliverdin Reductase expression in the Choroid Plexus after Subarachnoid Hemorrhage
SN  - 9788088064350
KW  - Hemoxygenase-1
KW  - biliverdin reductase
KW  - subarachnoidal hemorrhage
KW  - blood-CSF barrier
KW  - plexus choroideus
N2  - Subarachnoid hemorrhage (SAH) is a form of hemorrhagic stroke, associated with significant morbidity and mortality of affected patients. Complications after SAH include seizures, neurological deficits and an edematous state resulting from affected cerebrospinal fluid (CSF) circulatory dynamics. Blood clot degradation leads to activation of enzymes Hemeoxygenase-1 (HO-1) and Biliverdin reductase (BVR). The aim of our study was to describe the expression of HO-1 and BVR in the choroid plexus (CP) after SAH. Wistar rats (n=34; male) were used in our experiment. Injection of autologous arterial blood (SAH group) or artificial CSF (control group) to the cisterna magna was performed and rats were left to survive for 1, 3 and 7 days. SAH and control animals were sacrificed together with naive rats and coronal cryostat sections of the brain were prepared. Immunohistochemical detection of HO-1 and BVR enzymes in CP was performed, quantified and statistically analyzed. HO-1 was expressed in cuboidal cells (CC) and epiplexus cells (EC) in all groups of animals. After SAH, a higher statistically significant increase of HO-1 was observed in the CP compared with control and naive animals. Moreover, SAH induced increased number of HO-1+ EC following the time of survival. BVR was found in erythrocytes forming the clot on CP in animals 1 day after SAH. Subsequent survival times showed clot resolution and increased BVR expression in CC. An increased expression of HO-1 and BVR in CP was observed after SAH, suggesting possible disruption of CSF homeostasis and hydrocephalus formation.
ER  -

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