Phosphorylation of STAT3 at Serine 727 and its nuclear
translocation indicates direct and indirect induction of
regeneration program in the rat dorsal root ganglia neurons

k 2017

Phosphorylation of STAT3 at Serine 727 and its nuclear translocation indicates direct and indirect induction of regeneration program in the rat dorsal root ganglia neurons

KOKOŠOVÁ, Viktória, Ilona KLUSÁKOVÁ, Ivana HRADILOVÁ SVÍŽENSKÁ a Petr DUBOVÝ

Základní údaje

Originální název

Phosphorylation of STAT3 at Serine 727 and its nuclear translocation indicates direct and indirect induction of regeneration program in the rat dorsal root ganglia neurons

Autoři

KOKOŠOVÁ, Viktória (703 Slovensko), Ilona KLUSÁKOVÁ (203 Česká republika), Ivana HRADILOVÁ SVÍŽENSKÁ (203 Česká republika) a Petr DUBOVÝ (203 Česká republika, garant, domácí)

Vydání

Morphology 2017, 2017

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

30103 Neurosciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14110/17:00101339

Organizační jednotka

Lékařská fakulta

ISBN

978-80-263-1315-1

Klíčová slova česky

regenerační program; neurony; spinální ganglia

Klíčová slova anglicky

regeneration program; neurons; spinal ganglia

Příznaky

Mezinárodní význam

Změněno: 5. 12. 2018 09:56, prof. RNDr. Petr Dubový, CSc.

Anotace

V originále

Phosphorylation of STAT3 at Serine 727 and its nuclear translocation indicates direct and indirect induction of regeneration program in the rat dorsal root ganglia neurons Kokošová V., Klusáková I., Hradilová-Svíženská I., Dubový P. Department of Anatomy, Faculty of Medicine, Masaryk University, Brno, Czech Republic Phosphorylation of STAT3 on serine 727 (pSTAT3-S727) can modulate transcriptional activity of STAT3 phosphorylated on position of Tyrosine 705 (pSTAT3-Y705). Evidence of pSTAT3-S727 expression was not yet investigated in the dorsal root ganglia (DRG) neurons. Sixteen rats of Wistar line (male, 250-300g) were divided into four groups. The right sciatic nerve of the first group was compressed by triple ligatures (SNC, n=4), the sciatic nerve of the second group was cut and ligated to avoid spontaneous reinnervation (CSNT, n=4), and the sciatic nerve of the third group rats was only exposed without any damage (sham-operation). All rats were left to survive for 7 days. Rats without any surgical intervention were used as a naive control. Animals were perfused by Zamboni fixation and DRG were removed bilaterally in segments L4 and C6-C7. Expression of pSTAT3-S727 was detected by indirect immunohistochemical method and analyzed by image analysis program (NIS Elements, Nikon). Low intensity of pSTAT3-S727 immunofluorescence (IF) was measured in the neuronal nuclei of the lumbar and cervical DRG of the naive rats. Significant increased intensity of IF was found bilaterally in neuronal nuclei of DRG at L4 as well as C6-C7 segments after SNC and CSNT when compared with DRG from naive or sham-operated animals. The present results of pSTAT3-S727 expression in DRG neurons correlate well with our published results of IL-6, IL-6R and pSTAT3-Y705 elevation in both lumbar and cervical segments after unilateral sciatic nerve injury and support the idea of spreading inflammatory signaling alongside the spinal cord. Supported by grant 16-08508S of The Czech Science Foundation.

Návaznosti

GA16-08508S, projekt VaV

Název: Zvýšení endogenního regeneračního programu a jeho aktivace zprostředkovaná cytokiny/chemokiny v neuronech ganglií bez spojení s poškozeným nervem (Akronym: ERP)

Investor: Grantová agentura ČR, Zvýšení endogenního regeneračního programu a jeho aktivace zprostředkovaná cytokiny/chemokiny

Citovat

KOKOŠOVÁ, Viktória, Ilona KLUSÁKOVÁ, Ivana HRADILOVÁ SVÍŽENSKÁ a Petr DUBOVÝ. Phosphorylation of STAT3 at Serine 727 and its nuclear translocation indicates direct and indirect induction of regeneration program in the rat dorsal root ganglia neurons. In Morphology 2017. 2017. ISBN 978-80-263-1315-1.

@proceedings{1466517,
   author = {Kokošová, Viktória and Klusáková, Ilona and Hradilová Svíženská, Ivana and Dubový, Petr},
   booktitle = {Morphology 2017},
   keywords = {regeneration program; neurons; spinal ganglia},
   language = {eng},
   isbn = {978-80-263-1315-1},
   title = {Phosphorylation of STAT3 at Serine 727 and its nuclear translocation indicates direct and indirect induction of regeneration program in the rat dorsal root ganglia neurons},
   url = {http://www.morphology2017.cz/programme},
   year = {2017}
}

TY  - CONF
ID  - 1466517
AU  - Kokošová, Viktória - Klusáková, Ilona - Hradilová Svíženská, Ivana - Dubový, Petr
PY  - 2017
TI  - Phosphorylation of STAT3 at Serine 727 and its nuclear translocation indicates direct and indirect induction of regeneration program in the rat dorsal root ganglia neurons
SN  - 9788026313151
KW  - regeneration program
KW  - neurons
KW  - spinal ganglia
UR  - http://www.morphology2017.cz/programme
N2  - Phosphorylation of STAT3 at Serine 727 and its nuclear translocation indicates direct and indirect induction of regeneration program in the rat dorsal root ganglia neurons Kokošová V., Klusáková I., Hradilová-Svíženská I., Dubový P. Department of Anatomy, Faculty of Medicine, Masaryk University, Brno, Czech Republic Phosphorylation of STAT3 on serine 727 (pSTAT3-S727) can modulate transcriptional activity of STAT3 phosphorylated on position of Tyrosine 705 (pSTAT3-Y705). Evidence of pSTAT3-S727 expression was not yet investigated in the dorsal root ganglia (DRG) neurons. Sixteen rats of Wistar line (male, 250-300g) were divided into four groups. The right sciatic nerve of the first group was compressed by triple ligatures (SNC, n=4), the sciatic nerve of the second group was cut and ligated to avoid spontaneous reinnervation (CSNT, n=4), and the sciatic nerve of the third group rats was only exposed without any damage (sham-operation). All rats were left to survive for 7 days. Rats without any surgical intervention were used as a naive control. Animals were perfused by Zamboni fixation and DRG were removed bilaterally in segments L4 and C6-C7. Expression of pSTAT3-S727 was detected by indirect immunohistochemical method and analyzed by image analysis program (NIS Elements, Nikon). Low intensity of pSTAT3-S727 immunofluorescence (IF) was measured in the neuronal nuclei of the lumbar and cervical DRG of the naive rats. Significant increased intensity of IF was found bilaterally in neuronal nuclei of DRG at L4 as well as C6-C7 segments after SNC and CSNT when compared with DRG from naive or sham-operated animals. The present results of pSTAT3-S727 expression in DRG neurons correlate well with our published results of IL-6, IL-6R and pSTAT3-Y705 elevation in both lumbar and cervical segments after unilateral sciatic nerve injury and support the idea of spreading inflammatory signaling alongside the spinal cord. Supported by grant 16-08508S of The Czech Science Foundation.
ER  -

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