KADEŘÁVEK, Pavel, F. FERRAGE, G. BODENHAUSEN a D. KURZBACH. High-Resolution NMR of Folded Proteins in Hyperpolarized Physiological Solvents. Chemistry - A European Journal. WEINHEIM: Wiley, 2018, roč. 24, č. 51, s. 13418-13423. ISSN 0947-6539. Dostupné z: https://dx.doi.org/10.1002/chem.201802885. |
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@article{1468956, author = {Kadeřávek, Pavel and Ferrage, F. and Bodenhausen, G. and Kurzbach, D.}, article_location = {WEINHEIM}, article_number = {51}, doi = {http://dx.doi.org/10.1002/chem.201802885}, keywords = {dissolution DNP; hyperpolarized water; cross-relaxation effect; protein NMR spectroscopy; proton exchange}, language = {eng}, issn = {0947-6539}, journal = {Chemistry - A European Journal}, title = {High-Resolution NMR of Folded Proteins in Hyperpolarized Physiological Solvents}, url = {https://onlinelibrary.wiley.com/doi/full/10.1002/chem.201802885}, volume = {24}, year = {2018} }
TY - JOUR ID - 1468956 AU - Kadeřávek, Pavel - Ferrage, F. - Bodenhausen, G. - Kurzbach, D. PY - 2018 TI - High-Resolution NMR of Folded Proteins in Hyperpolarized Physiological Solvents JF - Chemistry - A European Journal VL - 24 IS - 51 SP - 13418-13423 EP - 13418-13423 PB - Wiley SN - 09476539 KW - dissolution DNP KW - hyperpolarized water KW - cross-relaxation effect KW - protein NMR spectroscopy KW - proton exchange UR - https://onlinelibrary.wiley.com/doi/full/10.1002/chem.201802885 N2 - Hyperpolarized 2D exchange spectroscopy (HYPEX) to obtain high-resolution nuclear magnetic resonance (NMR) spectra of folded proteins under near-physiological conditions is reported. The technique is based on hyperpolarized water, which is prepared by dissolution dynamic nuclear polarization and mixed in situ in an NMR spectrometer with a protein in a physiological saline buffer at body temperature. Rapid exchange of labile protons with the hyperpolarized solvent, combined with cross-relaxation effects (NOEs), leads to boosted signal intensities for many amide H-1-N-15 correlations in the protein ubiquitin. As the introduction of hyperpolarization to the target protein is mediated via the solvent, the method is applicable to a broad spectrum of target molecules. ER -
KADEŘÁVEK, Pavel, F. FERRAGE, G. BODENHAUSEN a D. KURZBACH. High-Resolution NMR of Folded Proteins in Hyperpolarized Physiological Solvents. \textit{Chemistry - A European Journal}. WEINHEIM: Wiley, 2018, roč.~24, č.~51, s.~13418-13423. ISSN~0947-6539. Dostupné z: https://dx.doi.org/10.1002/chem.201802885.
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