VOJTOVÁ, Lucy, Tomáš ZIKMUND, Veronika PAVLIŇÁKOVÁ, Jakub ŠALPLACHTA, Dominika KALASOVÁ, EVa PROSECKÁ, Jana BRTNÍKOVÁ, Jakub ŽÍDEK, David PAVLIŇÁK and Jozef KAISER. The 3D imaging of mesenchymal stem cells on porous scaffolds using high-contrasted x-ray computed nanotomography. Journal of Microscopy. Hoboken: WILEY-BLACKWELL, 2019, vol. 273, No 3, p. 169-177. ISSN 0022-2720. Available from: https://dx.doi.org/10.1111/jmi.12771.
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Basic information
Original name The 3D imaging of mesenchymal stem cells on porous scaffolds using high-contrasted x-ray computed nanotomography
Authors VOJTOVÁ, Lucy (203 Czech Republic, guarantor), Tomáš ZIKMUND (203 Czech Republic), Veronika PAVLIŇÁKOVÁ (203 Czech Republic), Jakub ŠALPLACHTA (203 Czech Republic), Dominika KALASOVÁ (203 Czech Republic), EVa PROSECKÁ (203 Czech Republic), Jana BRTNÍKOVÁ (203 Czech Republic), Jakub ŽÍDEK (203 Czech Republic), David PAVLIŇÁK (203 Czech Republic, belonging to the institution) and Jozef KAISER (203 Czech Republic).
Edition Journal of Microscopy, Hoboken, WILEY-BLACKWELL, 2019, 0022-2720.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10300 1.3 Physical sciences
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW Full Text
Impact factor Impact factor: 1.575
RIV identification code RIV/00216224:14310/19:00108936
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1111/jmi.12771
UT WoS 000458426100002
Keywords in English biopolymeric scaffold;mesenchymal stem cells;SEM/EDX;tissue engineering; X-ray computed nanotomography
Tags rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 11/3/2020 15:39.
Abstract
This study presents an X-ray computed nanotomography (nano-CT) based, high-resolution imaging technique. Asample of highly porous collagen scaffold seeded with contrastedmesenchymalstemcells(MSC)was investigated by using lab-based nano-CT. The whole volume of the sample was analysed without its destruction. To evaluate the potential of nano-CT, a comparison measurement was done using a standard microscopy technique. Scanning electron microscopy (SEM) combined with energy dispersive X-ray analysis (EDX) established an extension and local accumulation of the contrasting agent – heavy metallic osmium tetroxide.
Links
ED2.1.00/03.0086, research and development projectName: Regionální VaV centrum pro nízkonákladové plazmové a nanotechnologické povrchové úpravy
LO1411, research and development projectName: Rozvoj centra pro nízkonákladové plazmové a nanotechnologické povrchové úpravy (Acronym: CEPLANT plus)
Investor: Ministry of Education, Youth and Sports of the CR
LQ1601, research and development projectName: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
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