CAPILLARY ELECTROPHORETIC ANALYSIS OF EXHALED BREATH CONDENSATE
IN THE DIAGNOSIS OF GASTROESOPHAGEAL REFLUX DISEASE

J 2018

CAPILLARY ELECTROPHORETIC ANALYSIS OF EXHALED BREATH CONDENSATE IN THE DIAGNOSIS OF GASTROESOPHAGEAL REFLUX DISEASE

KUBÁŇ, Petr, Pavol ĎURČ, Júlia LAČNÁ, Michal GREGUŠ, František FORET et. al.

Základní údaje

Originální název

CAPILLARY ELECTROPHORETIC ANALYSIS OF EXHALED BREATH CONDENSATE IN THE DIAGNOSIS OF GASTROESOPHAGEAL REFLUX DISEASE

Autoři

KUBÁŇ, Petr (203 Česká republika, garant, domácí), Pavol ĎURČ (703 Slovensko, domácí), Júlia LAČNÁ (703 Slovensko, domácí), Michal GREGUŠ (703 Slovensko, domácí), František FORET (203 Česká republika, domácí), Jiří DOLINA (203 Česká republika, domácí), Štefan KONEČNÝ (703 Slovensko, domácí), Martina DOUBKOVÁ (203 Česká republika, domácí), Dagmar KINDLOVÁ (203 Česká republika, domácí), Eva POKOJOVÁ (203 Česká republika, domácí) a Jana SKŘIČKOVÁ (203 Česká republika, domácí)

Vydání

HUNGARIAN JOURNAL OF INDUSTRY AND CHEMISTRY, WARSAW, DE GRUYTER POLAND SP ZOO, 2018, 0133-0276

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Polsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 0.084 v roce 2002

Kód RIV

RIV/00216224:14740/18:00106949

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1515/hjic-2018-0006

UT WoS

000440079700006

Klíčová slova anglicky

capillary electrophoresis; exhaled breath condensate; ionic analysis; diagnosis; gastroesophageal reflux disease

Štítky

14110213, 14110215, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 4. 3. 2019 12:28, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

In this work, capillary electrophoresis with contactless conductometric detection (CCD) was used for the analysis of the ionic content of exhaled breath condensate (EBC) to differentiate between healthy individuals and patients with gastroesophageal reflux disease (GERD). The exhaled breath condensate was collected using a miniature sample collection device and the content analyzed using a separation electrolyte composed of 20 mM 2-(N-morpholino)ethanesulfonic acid, 20 mM L-histidine, 2 mM 18-Crown-6 and 30 mu M cetyltrimethylammonium bromide. The separation of anions took less than 2.5 minutes, while the cations were separated in less than 1.5 minutes. The most significantly elevated ions in the group of patients suffering from gastroesophageal reflux disease were chloride, nitrate, propionate and butyrate. Although the number of subjects was too small to draw definite conclusions with regard to the discriminatory power of these ions, the pilot data are promising for EBC as a useful non-invasive alternative for other methods used in the diagnosis of gastroesophageal reflux disease.

Návaznosti

NV17-31945A, projekt VaV

Název: Vývoj neinvazivní diagnostiky extraezofageálních projevů refluxní choroby jícnu

Citovat

KUBÁŇ, Petr, Pavol ĎURČ, Júlia LAČNÁ, Michal GREGUŠ, František FORET, Jiří DOLINA, Štefan KONEČNÝ, Martina DOUBKOVÁ, Dagmar KINDLOVÁ, Eva POKOJOVÁ a Jana SKŘIČKOVÁ. CAPILLARY ELECTROPHORETIC ANALYSIS OF EXHALED BREATH CONDENSATE IN THE DIAGNOSIS OF GASTROESOPHAGEAL REFLUX DISEASE. HUNGARIAN JOURNAL OF INDUSTRY AND CHEMISTRY. WARSAW: DE GRUYTER POLAND SP ZOO, 2018, roč. 46, č. 1, s. 23-27. ISSN 0133-0276. Dostupné z: https://dx.doi.org/10.1515/hjic-2018-0006.

@article{1472910,
   author = {Kubáň, Petr and Ďurč, Pavol and Lačná, Júlia and Greguš, Michal and Foret, František and Dolina, Jiří and Konečný, Štefan and Doubková, Martina and Kindlová, Dagmar and Pokojová, Eva and Skřičková, Jana},
   article_location = {WARSAW},
   article_number = {1},
   doi = {http://dx.doi.org/10.1515/hjic-2018-0006},
   keywords = {capillary electrophoresis; exhaled breath condensate; ionic analysis; diagnosis; gastroesophageal reflux disease},
   language = {eng},
   issn = {0133-0276},
   journal = {HUNGARIAN JOURNAL OF INDUSTRY AND CHEMISTRY},
   title = {CAPILLARY ELECTROPHORETIC ANALYSIS OF EXHALED BREATH CONDENSATE IN THE DIAGNOSIS OF GASTROESOPHAGEAL REFLUX DISEASE},
   url = {https://content.sciendo.com/view/journals/hjic/46/1/article-p23.xml},
   volume = {46},
   year = {2018}
}

TY  - JOUR
ID  - 1472910
AU  - Kubáň, Petr - Ďurč, Pavol - Lačná, Júlia - Greguš, Michal - Foret, František - Dolina, Jiří - Konečný, Štefan - Doubková, Martina - Kindlová, Dagmar - Pokojová, Eva - Skřičková, Jana
PY  - 2018
TI  - CAPILLARY ELECTROPHORETIC ANALYSIS OF EXHALED BREATH CONDENSATE IN THE DIAGNOSIS OF GASTROESOPHAGEAL REFLUX DISEASE
JF  - HUNGARIAN JOURNAL OF INDUSTRY AND CHEMISTRY
VL  - 46
IS  - 1
SP  - 23-27
EP  - 23-27
PB  - DE GRUYTER POLAND SP ZOO
SN  - 01330276
KW  - capillary electrophoresis
KW  - exhaled breath condensate
KW  - ionic analysis
KW  - diagnosis
KW  - gastroesophageal reflux disease
UR  - https://content.sciendo.com/view/journals/hjic/46/1/article-p23.xml
N2  - In this work, capillary electrophoresis with contactless conductometric detection (CCD) was used for the analysis of the ionic content of exhaled breath condensate (EBC) to differentiate between healthy individuals and patients with gastroesophageal reflux disease (GERD). The exhaled breath condensate was collected using a miniature sample collection device and the content analyzed using a separation electrolyte composed of 20 mM 2-(N-morpholino)ethanesulfonic acid, 20 mM L-histidine, 2 mM 18-Crown-6 and 30 mu M cetyltrimethylammonium bromide. The separation of anions took less than 2.5 minutes, while the cations were separated in less than 1.5 minutes. The most significantly elevated ions in the group of patients suffering from gastroesophageal reflux disease were chloride, nitrate, propionate and butyrate. Although the number of subjects was too small to draw definite conclusions with regard to the discriminatory power of these ions, the pilot data are promising for EBC as a useful non-invasive alternative for other methods used in the diagnosis of gastroesophageal reflux disease.
ER  -

KUBÁŇ, Petr, Pavol ĎURČ, Júlia LAČNÁ, Michal GREGUŠ, František FORET, Jiří DOLINA, Štefan KONEČNÝ, Martina DOUBKOVÁ, Dagmar KINDLOVÁ, Eva POKOJOVÁ a Jana SKŘIČKOVÁ. CAPILLARY ELECTROPHORETIC ANALYSIS OF EXHALED BREATH CONDENSATE IN THE DIAGNOSIS OF GASTROESOPHAGEAL REFLUX DISEASE. \textit{HUNGARIAN JOURNAL OF INDUSTRY AND CHEMISTRY}. WARSAW: DE GRUYTER POLAND SP ZOO, 2018, roč.~46, č.~1, s.~23-27. ISSN~0133-0276. Dostupné z: https://dx.doi.org/10.1515/hjic-2018-0006.

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