Bejel in Cuba: molecular identification of Treponema pallidum
subsp endemicum in patients diagnosed with venereal syphilis

J 2018

Bejel in Cuba: molecular identification of Treponema pallidum subsp endemicum in patients diagnosed with venereal syphilis

NODA, A.A., Linda GRILLOVÁ, R. LIENHARD, O. BLANCO, I. RODRIGUEZ et. al.

Základní údaje

Originální název

Bejel in Cuba: molecular identification of Treponema pallidum subsp endemicum in patients diagnosed with venereal syphilis

Autoři

NODA, A.A. (192 Kuba), Linda GRILLOVÁ (203 Česká republika, domácí), R. LIENHARD (756 Švýcarsko), O. BLANCO (192 Kuba), I. RODRIGUEZ (192 Kuba) a David ŠMAJS (203 Česká republika, garant, domácí)

Vydání

Clinical Microbiology and Infection, NJ USA, WILEY-BLACKWELL, 2018, 1198-743X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30303 Infectious Diseases

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 6.425

Kód RIV

RIV/00216224:14110/18:00101381

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.cmi.2018.02.006

UT WoS

000448188200019

Klíčová slova anglicky

Bejel; Cuba; Diagnosis; Molecular typing; Treponema pallidum subsp endemicum

Štítky

14110513, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 2. 2019 21:05, Soňa Böhmová

Anotace

V originále

Objectives: Bejel, caused by Treponema pallidum subsp. endemicum (TEN), was until now considered as a non-venereal disease endemic in areas with hot and dry climates. This study has identified TEN in clinical samples from Cuban patients previously diagnosed with syphilis. Methods: We performed sequencing-based molecular typing on 92 samples from Cuban individuals diagnosed with syphilis. Moreover, to differentiate T. pallidum subspecies, multi-locus sequence analysis (MLSA) was designed and was applied to suspicious samples. Results: Nine samples, from six patients, had a nucleotide sequence similarity (at all typing loci) to the Bosnia A genome, which is the infectious agent of bejel. Additionally, MLSA clearly supported a TEN classification for the treponemal samples. Clinical and epidemiological data from the six patients also suggested sexual transmission of bejel as well as the endemicity of this rare treponematosis in Cuba. Conclusions: Molecular identification of Treponema pallidum subsp. endemicum, the agent of bejel, in Cuban patients diagnosed with syphilis indicates the clear limitations of a diagnosis based exclusively on serology, geographical occurrence, clinical symptoms and anamnestic data. This finding has important implications for Global Public Health Systems, including paradigm changes regarding the location of endemic outbreaks, clinical aspects and transmission of this neglected disease. (C) 2018 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.

Návaznosti

GA17-25455S, projekt VaV

Název: Studium genomů patogenních treponem na základě analýzy jednotlivých buněk

Investor: Grantová agentura ČR, Studium genomů patogenních treponem na základě analýzy jednotlivých buněk

NV17-31333A, projekt VaV

Název: Vývoj nového typovacího systému pro původce syfilis, Treponema pallidum subsp. pallidum, zaměřeného na proteomické rozdíly

Citovat

NODA, A.A., Linda GRILLOVÁ, R. LIENHARD, O. BLANCO, I. RODRIGUEZ a David ŠMAJS. Bejel in Cuba: molecular identification of Treponema pallidum subsp endemicum in patients diagnosed with venereal syphilis. Clinical Microbiology and Infection. NJ USA: WILEY-BLACKWELL, 2018, roč. 24, č. 11, s. 1-5. ISSN 1198-743X. Dostupné z: https://dx.doi.org/10.1016/j.cmi.2018.02.006.

@article{1473176,
   author = {Noda, A.A. and Grillová, Linda and Lienhard, R. and Blanco, O. and Rodriguez, I. and Šmajs, David},
   article_location = {NJ USA},
   article_number = {11},
   doi = {http://dx.doi.org/10.1016/j.cmi.2018.02.006},
   keywords = {Bejel; Cuba; Diagnosis; Molecular typing; Treponema pallidum subsp endemicum},
   language = {eng},
   issn = {1198-743X},
   journal = {Clinical Microbiology and Infection},
   title = {Bejel in Cuba: molecular identification of Treponema pallidum subsp endemicum in patients diagnosed with venereal syphilis},
   volume = {24},
   year = {2018}
}

TY  - JOUR
ID  - 1473176
AU  - Noda, A.A. - Grillová, Linda - Lienhard, R. - Blanco, O. - Rodriguez, I. - Šmajs, David
PY  - 2018
TI  - Bejel in Cuba: molecular identification of Treponema pallidum subsp endemicum in patients diagnosed with venereal syphilis
JF  - Clinical Microbiology and Infection
VL  - 24
IS  - 11
SP  - 1-5
EP  - 1-5
PB  - WILEY-BLACKWELL
SN  - 1198743X
KW  - Bejel
KW  - Cuba
KW  - Diagnosis
KW  - Molecular typing
KW  - Treponema pallidum subsp endemicum
N2  - Objectives: Bejel, caused by Treponema pallidum subsp. endemicum (TEN), was until now considered as a non-venereal disease endemic in areas with hot and dry climates. This study has identified TEN in clinical samples from Cuban patients previously diagnosed with syphilis. Methods: We performed sequencing-based molecular typing on 92 samples from Cuban individuals diagnosed with syphilis. Moreover, to differentiate T. pallidum subspecies, multi-locus sequence analysis (MLSA) was designed and was applied to suspicious samples. Results: Nine samples, from six patients, had a nucleotide sequence similarity (at all typing loci) to the Bosnia A genome, which is the infectious agent of bejel. Additionally, MLSA clearly supported a TEN classification for the treponemal samples. Clinical and epidemiological data from the six patients also suggested sexual transmission of bejel as well as the endemicity of this rare treponematosis in Cuba. Conclusions: Molecular identification of Treponema pallidum subsp. endemicum, the agent of bejel, in Cuban patients diagnosed with syphilis indicates the clear limitations of a diagnosis based exclusively on serology, geographical occurrence, clinical symptoms and anamnestic data. This finding has important implications for Global Public Health Systems, including paradigm changes regarding the location of endemic outbreaks, clinical aspects and transmission of this neglected disease. (C) 2018 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
ER  -

NODA, A.A., Linda GRILLOVÁ, R. LIENHARD, O. BLANCO, I. RODRIGUEZ a David ŠMAJS. Bejel in Cuba: molecular identification of Treponema pallidum subsp endemicum in patients diagnosed with venereal syphilis. \textit{Clinical Microbiology and Infection}. NJ USA: WILEY-BLACKWELL, 2018, roč.~24, č.~11, s.~1-5. ISSN~1198-743X. Dostupné z: https://dx.doi.org/10.1016/j.cmi.2018.02.006.

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