Characterization of PluLec, a novel galactose-specific lectin
from Photorhabdus luminescens

a 2018

Characterization of PluLec, a novel galactose-specific lectin from Photorhabdus luminescens

KORSÁK, Marek, Josef HOUSER, Eva FUJDIAROVÁ, Marie POKORNÁ, Jan KOMÁREK et. al.

Základní údaje

Originální název

Characterization of PluLec, a novel galactose-specific lectin from Photorhabdus luminescens

Autoři

KORSÁK, Marek (703 Slovensko, domácí), Josef HOUSER (203 Česká republika, domácí), Eva FUJDIAROVÁ (203 Česká republika, domácí), Marie POKORNÁ (203 Česká republika, domácí), Jan KOMÁREK (203 Česká republika, domácí) a Michaela WIMMEROVÁ (203 Česká republika, garant, domácí)

Vydání

XIX. setkání biochemiků a molekulárních biologů, 2018

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/18:00104710

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-210-9069-9

Klíčová slova česky

Photorhabdus luminescens;lektin;

Klíčová slova anglicky

Photorhabdus luminescens;lectin

Změněno: 29. 11. 2018 16:49, Mgr. Marek Korsák

Anotace

V originále

Lectins are proteins of non-immune origin capable of binding carbohydrates reversibly and with high specificity. They are found in all known living organisms. Unlike antibodies, they are not product of immune response and do not possess any enzymatic activity. Lectins are involved in recognition events in various physiological and pathological processes (e.g. cell communication, adhesion, migration, pathogen recognition, immune response modulation). Lectins are commonly used for characterization of carbohydrate structures, for purification of glycoproteins and to specifically label the cell surface structures. They are also used for separation and cell typing. Photorhabdus luminescens is a naturally bioluminescent Gram-negative bacterium and an insect pathogen, which symbiotically lives in Heterorhabditidae nematodes. PluLec is a putative lectin from Photorhabdus luminescens and a homologue of PA-IL lectin, which is D-galactose specific, Ca2+ dependent, cytotoxic lectin from opportunistic pathogen Pseudomonas aeruginosa, involved in facilitating infection in patients with compromised immunity. This research is focused on cloning, production and study of recombinant protein PluLec using various methods for structural and functional characterization, namely dynamic light scattering, circular dichroism, differential scanning fluorimetry, hemagglutination, glycan array, surface plasmon resonance, analytical ultracentrifugation and protein X-ray crystallography. Detailed knowledge of the structure and function of PluLec can help to understand the mechanism of P. luminescens infection. The lectin might also find further application in biotechnological research.

Návaznosti

MUNI/A/1100/2017, interní kód MU

Název: Podpora biochemického výzkumu v roce 2018

Investor: Masarykova univerzita, Podpora biochemického výzkumu v roce 2018, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

KORSÁK, Marek, Josef HOUSER, Eva FUJDIAROVÁ, Marie POKORNÁ, Jan KOMÁREK a Michaela WIMMEROVÁ. Characterization of PluLec, a novel galactose-specific lectin from Photorhabdus luminescens. In XIX. setkání biochemiků a molekulárních biologů. 2018. ISBN 978-80-210-9069-9.

@proceedings{1473310,
   author = {Korsák, Marek and Houser, Josef and Fujdiarová, Eva and Pokorná, Marie and Komárek, Jan and Wimmerová, Michaela},
   booktitle = {XIX. setkání biochemiků a molekulárních biologů},
   keywords = {Photorhabdus luminescens;lectin},
   language = {eng},
   isbn = {978-80-210-9069-9},
   title = {Characterization of PluLec, a novel galactose-specific lectin from Photorhabdus luminescens},
   year = {2018}
}

TY  - CONF
ID  - 1473310
AU  - Korsák, Marek - Houser, Josef - Fujdiarová, Eva - Pokorná, Marie - Komárek, Jan - Wimmerová, Michaela
PY  - 2018
TI  - Characterization of PluLec, a novel galactose-specific lectin from Photorhabdus luminescens
SN  - 9788021090699
KW  - Photorhabdus luminescens;lectin
N2  - Lectins are proteins of non-immune origin capable of binding carbohydrates reversibly and with high specificity. They are found in all known living organisms. Unlike antibodies, they are not product of immune response and do not possess any enzymatic activity. Lectins are involved in recognition events in various physiological and pathological processes (e.g. cell communication, adhesion, migration, pathogen recognition, immune response modulation). Lectins are commonly used for characterization of carbohydrate structures, for purification of glycoproteins and to specifically label the cell surface structures. They are also used for separation and cell typing. Photorhabdus luminescens is a naturally bioluminescent Gram-negative bacterium and an insect pathogen, which symbiotically lives in Heterorhabditidae nematodes. PluLec is a putative lectin from Photorhabdus luminescens and a homologue of PA-IL lectin, which is D-galactose specific, Ca2+ dependent, cytotoxic lectin from opportunistic pathogen Pseudomonas aeruginosa, involved in facilitating infection in patients with compromised immunity. This research is focused on cloning, production and study of recombinant protein PluLec using various methods for structural and functional characterization, namely dynamic light scattering, circular dichroism, differential scanning fluorimetry, hemagglutination, glycan array, surface plasmon resonance, analytical ultracentrifugation and protein X-ray crystallography. Detailed knowledge of the structure and function of PluLec can help to understand the mechanism of P. luminescens infection. The lectin might also find further application in biotechnological research.
ER  -

KORSÁK, Marek, Josef HOUSER, Eva FUJDIAROVÁ, Marie POKORNÁ, Jan KOMÁREK a Michaela WIMMEROVÁ. Characterization of PluLec, a novel galactose-specific lectin from Photorhabdus luminescens. In \textit{XIX. setkání biochemiků a molekulárních biologů}. 2018. ISBN~978-80-210-9069-9.

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