Factors contributing to the coexistence of two reproductive
forms of Carassius gibelio in the Czech Republic.

a 2018

Factors contributing to the coexistence of two reproductive forms of Carassius gibelio in the Czech Republic.

PAKOSTA, Tomáš, Lukáš VETEŠNÍK, Pavel HYRŠL a Andrea VETEŠNÍKOVÁ ŠIMKOVÁ

Základní údaje

Originální název

Factors contributing to the coexistence of two reproductive forms of Carassius gibelio in the Czech Republic.

Autoři

PAKOSTA, Tomáš (203 Česká republika, domácí), Lukáš VETEŠNÍK (203 Česká republika, domácí), Pavel HYRŠL (203 Česká republika, domácí) a Andrea VETEŠNÍKOVÁ ŠIMKOVÁ (203 Česká republika, garant, domácí)

Vydání

7th Workshop of the European Centre of IchthyoParasitology, 2018

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10613 Zoology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/18:00101390

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-210-9079-8

Klíčová slova anglicky

Carassius gibelio; MHC; gynogenetic; immunocompetence; host-parasite dynamics

Změněno: 3. 12. 2018 09:10, Mgr. Tomáš Pakosta

Anotace

V originále

The first records of Carassius auratus gibelio species arose in 1976. Since 1990 the transformation from originally triploid (likely gynogenetic) towards the mixed diploid-polyploid population (with coexisting gynogenetic and sexual forms) was reported. This unique fish species, which originated from Asia, is characterized by high environmental tolerance and adaptability. Therefore, it is a perfect model of a successful, invasive species. However, until now, the potential differences in physiology, immunity and/or parasitic between two coexisting reproductive forms were not fully investigated. The aim of this study was to investigate the parasite load and the selected physiological and immune parameters in two reproductive forms of C. auratus gibelio. According to mtDNA analysis, C. gibelio, which is a target of our study, is the most common form of Carassius auratus complex in the Czech Republic. Apart from C. auratus gibelio there are also other three forms reported in Czech Republic in low frequency i.e. C. auratus auratus, C. auratus langsdorfii and C. auratus M-line. In the frame of our four-year study, 22 different species of metazoan parasites were found on 195 fish individuals investigated. Monogenea represented the most frequent group of parasites in both diploid likely sexual females and males, and triploid likely gynogenetic females. From all parasite species, 3 species of Monogenea, namely in Dactylogyrus dulkeiti, Dactylogyrus anchoratus and Gyrodactylus sprostonae were found in the high prevalence and the highest intensity of infection. This pattern was recognized in both reproduction forms. We also found that both sexual individuals and gynogenetic females were parasitized more in the last year in comparison with three previous years. We did not confirm the prediction of Red Queen hypothesis as we did not find significantly higher level of infection in gynogenetic females compared to sexual forms in any of four years studied. Concerning physiological parameters studied, we confirmed statistically higher erythrocyte count in diploid form when compared to triploid form. On the other hand, higher level of haematocrit was found in diploid males compared to both groups of females. From the immunity point of view, we did not observe any significant difference between reproductive forms of C. auratus gibelio. Nevertheless, difference in some physiological and immune parameters were found among the specimens collected in different years. Activity of oxidative burst reached significantly lower values in the last year of study for both forms. On the other hand, in the same year the activity of the complement was significantly higher when compared to other 3 years. Condition factor and gonado-somatic factor were lower in diploid males when compared to sexual and asexual females. We also reported higher value of spleen-somatic index (considered as an indicator of immunocompetence) of diploid males when compared to both females. Thus, it seems that the high investment in gonad development in both gynogenetic and sexual females is compensated by low investment in immunocompetence. This result may indicate that investment into traits related to the maintenance, reproduction and immunity are determined rather by sex than by different ploidy level likely related to different modes of reproduction (gynogenetic vs. sexual). This may contribute to the coexistence of two reproduction forms of C. gibelio living in the same environment.

Návaznosti

GBP505/12/G112, projekt VaV

Název: ECIP - Evropské centrum ichtyoparazitologie

Investor: Grantová agentura ČR, ECIP - Evropské centrum ichtyoparazitologie

Citovat

PAKOSTA, Tomáš, Lukáš VETEŠNÍK, Pavel HYRŠL a Andrea VETEŠNÍKOVÁ ŠIMKOVÁ. Factors contributing to the coexistence of two reproductive forms of Carassius gibelio in the Czech Republic. In 7th Workshop of the European Centre of IchthyoParasitology. 2018. ISBN 978-80-210-9079-8.

@proceedings{1474478,
   author = {Pakosta, Tomáš and Vetešník, Lukáš and Hyršl, Pavel and Vetešníková Šimková, Andrea},
   booktitle = {7th Workshop of the European Centre of IchthyoParasitology},
   keywords = {Carassius gibelio; MHC; gynogenetic; immunocompetence; host-parasite dynamics},
   language = {eng},
   isbn = {978-80-210-9079-8},
   title = {Factors contributing to the coexistence of two reproductive forms of Carassius gibelio in the Czech Republic.},
   year = {2018}
}

TY  - CONF
ID  - 1474478
AU  - Pakosta, Tomáš - Vetešník, Lukáš - Hyršl, Pavel - Vetešníková Šimková, Andrea
PY  - 2018
TI  - Factors contributing to the coexistence of two reproductive forms of Carassius gibelio in the Czech Republic.
SN  - 9788021090798
KW  - Carassius gibelio
KW  - MHC
KW  - gynogenetic
KW  - immunocompetence
KW  - host-parasite dynamics
N2  - The first records of Carassius auratus gibelio species arose in 1976. Since 1990 the transformation from originally triploid (likely gynogenetic) towards the mixed diploid-polyploid population (with coexisting gynogenetic and sexual forms) was reported. This unique fish species, which originated from Asia, is characterized by high environmental tolerance and adaptability. Therefore, it is a perfect model of a successful, invasive species. However, until now, the potential differences in physiology, immunity and/or parasitic between two coexisting reproductive forms were not fully investigated. The aim of this study was to investigate the parasite load and the selected physiological and immune parameters in two reproductive forms of C. auratus gibelio. According to mtDNA analysis, C. gibelio, which is a target of our study, is the most common form of Carassius auratus complex in the Czech Republic. Apart from C. auratus gibelio there are also other three forms reported in Czech Republic in low frequency i.e. C. auratus auratus, C. auratus langsdorfii and C. auratus M-line. In the frame of our four-year study, 22 different species of metazoan parasites were found on 195 fish individuals investigated. Monogenea represented the most frequent group of parasites in both diploid likely sexual females and males, and triploid likely gynogenetic females. From all parasite species, 3 species of Monogenea, namely in Dactylogyrus dulkeiti, Dactylogyrus anchoratus and Gyrodactylus sprostonae were found in the high prevalence and the highest intensity of infection. This pattern was recognized in both reproduction forms. We also found that both sexual individuals and gynogenetic females were parasitized more in the last year in comparison with three previous years. We did not confirm the prediction of Red Queen hypothesis as we did not find significantly higher level of infection in gynogenetic females compared to sexual forms in any of four years studied. Concerning physiological parameters studied, we confirmed statistically higher erythrocyte count in diploid form when compared to triploid form. On the other hand, higher level of haematocrit was found in diploid males compared to both groups of females. From the immunity point of view, we did not observe any significant difference between reproductive forms of C. auratus gibelio. Nevertheless, difference in some physiological and immune parameters were found among the specimens collected in different years. Activity of oxidative burst reached significantly lower values in the last year of study for both forms. On the other hand, in the same year the activity of the complement was significantly higher when compared to other 3 years. Condition factor and gonado-somatic factor were lower in diploid males when compared to sexual and asexual females. We also reported higher value of spleen-somatic index (considered as an indicator of immunocompetence) of diploid males when compared to both females. Thus, it seems that the high investment in gonad development in both gynogenetic and sexual females is compensated by low investment in immunocompetence. This result may indicate that investment into traits related to the maintenance, reproduction and immunity are determined rather by sex than by different ploidy level likely related to different modes of reproduction (gynogenetic vs. sexual). This may contribute to the coexistence of two reproduction forms of C. gibelio living in the same environment.
ER  -

PAKOSTA, Tomáš, Lukáš VETEŠNÍK, Pavel HYRŠL a Andrea VETEŠNÍKOVÁ ŠIMKOVÁ. Factors contributing to the coexistence of two reproductive forms of Carassius gibelio in the Czech Republic. In \textit{7th Workshop of the European Centre of IchthyoParasitology}. 2018. ISBN~978-80-210-9079-8.

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