Neuroinflammatory regulations of regenerative program in the
dorsal root ganglia neurons non-associated with injured nerve

a 2018

Neuroinflammatory regulations of regenerative program in the dorsal root ganglia neurons non-associated with injured nerve

DUBOVÝ, Petr, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ, Václav BRÁZDA, Marek JOUKAL et. al.

Základní údaje

Originální název

Neuroinflammatory regulations of regenerative program in the dorsal root ganglia neurons non-associated with injured nerve

Autoři

DUBOVÝ, Petr (203 Česká republika, domácí), Ivana HRADILOVÁ SVÍŽENSKÁ (203 Česká republika, domácí), Ilona KLUSÁKOVÁ (203 Česká republika, domácí), Václav BRÁZDA (203 Česká republika, domácí) a Marek JOUKAL (203 Česká republika, garant, domácí)

Vydání

Morphology 2018, 2018

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30103 Neurosciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/18:00101399

Organizační jednotka

Lékařská fakulta

ISBN

978-80-88064-35-0

Klíčová slova anglicky

regenerative program; remote DRG; IL-6

Změněno: 5. 12. 2018 09:49, doc. MUDr. Marek Joukal, Ph.D.

Anotace

V originále

We found earlier that unilateral sciatic nerve injury can induce inflammatory reactions bilaterally not only in the dorsal root ganglion (DRG) neurons associated with L4-L5 spinal cord segments but also in remote cervical DRG. This systemic neuroinflammatory reaction of DRG neurons can be related with their pro-regenerative state after peripheral nerve injury. To verify our hypothesis, we investigated axon regeneration distal to the rat ulnar nerve crush after prior sciatic nerve injury, and after intrathecal application of IL-6 or AG490 inhibitor of JAK2 in comparison to vehiculum. A role of IL-6 in activation of pro-regenerative state of DRG neurons was also tested in IL-6 -/- mice. We found that sciatic nerve lesion for 7 days increased the distance of regenerated axons after the ulnar nerve crush. In addition, simultaneous intrathecal application of IL-6 with the ulnar nerve crush extended regenerated axons, too. In contrast, inhibition of STAT3 phosphorylation by JAK2 resulted in significantly reduced lengths of regenerated axon. A regeneration of axons distal to the ulnar nerve crush after prior sciatic nerve injury was significantly reduced in IL-6 -/- mice. The results presented here confirmed that unilateral sciatic nerve injury can induce pro-regenerative state of the cervical DRG neurons non-associated with injured nerve. This reflects a systemic reaction of the DRG neurons alongside neuroaxis to unilateral nerve injury mediated by IL-6 released into the paraspinal intrathecal space.

Návaznosti

GA16-08508S, projekt VaV

Název: Zvýšení endogenního regeneračního programu a jeho aktivace zprostředkovaná cytokiny/chemokiny v neuronech ganglií bez spojení s poškozeným nervem (Akronym: ERP)

Investor: Grantová agentura ČR, Zvýšení endogenního regeneračního programu a jeho aktivace zprostředkovaná cytokiny/chemokiny

Citovat

DUBOVÝ, Petr, Ivana HRADILOVÁ SVÍŽENSKÁ, Ilona KLUSÁKOVÁ, Václav BRÁZDA a Marek JOUKAL. Neuroinflammatory regulations of regenerative program in the dorsal root ganglia neurons non-associated with injured nerve. In Morphology 2018. 2018. ISBN 978-80-88064-35-0.

@proceedings{1475168,
   author = {Dubový, Petr and Hradilová Svíženská, Ivana and Klusáková, Ilona and Brázda, Václav and Joukal, Marek},
   booktitle = {Morphology 2018},
   keywords = {regenerative program; remote DRG; IL-6},
   language = {eng},
   isbn = {978-80-88064-35-0},
   title = {Neuroinflammatory regulations of regenerative program in the dorsal root ganglia neurons non-associated with injured nerve},
   year = {2018}
}

TY  - CONF
ID  - 1475168
AU  - Dubový, Petr - Hradilová Svíženská, Ivana - Klusáková, Ilona - Brázda, Václav - Joukal, Marek
PY  - 2018
TI  - Neuroinflammatory regulations of regenerative program in the dorsal root ganglia neurons non-associated with injured nerve
SN  - 9788088064350
KW  - regenerative program
KW  - remote DRG
KW  - IL-6
N2  - We found earlier that unilateral sciatic nerve injury can induce inflammatory reactions bilaterally not only in the dorsal root ganglion (DRG) neurons associated with L4-L5 spinal cord segments but also in remote cervical DRG. This systemic neuroinflammatory reaction of DRG neurons can be related with their pro-regenerative state after peripheral nerve injury. To verify our hypothesis, we investigated axon regeneration distal to the rat ulnar nerve crush after prior sciatic nerve injury, and after intrathecal application of IL-6 or AG490 inhibitor of JAK2 in comparison to vehiculum. A role of IL-6 in activation of pro-regenerative state of DRG neurons was also tested in IL-6 -/- mice. We found that sciatic nerve lesion for 7 days increased the distance of regenerated axons after the ulnar nerve crush. In addition, simultaneous intrathecal application of IL-6 with the ulnar nerve crush extended regenerated axons, too. In contrast, inhibition of STAT3 phosphorylation by JAK2 resulted in significantly reduced lengths of regenerated axon. A regeneration of axons distal to the ulnar nerve crush after prior sciatic nerve injury was significantly reduced in IL-6 -/- mice. The results presented here confirmed that unilateral sciatic nerve injury can induce pro-regenerative state of the cervical DRG neurons non-associated with injured nerve. This reflects a systemic reaction of the DRG neurons alongside neuroaxis to unilateral nerve injury mediated by IL-6 released into the paraspinal intrathecal space.
ER  -

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