EXPRESSION OF REGENERATION-ASSOCIATED PROTEINS IN THE PRIMARY
SENSORY NEURONS AND REGENERATING AXONS AFTER NERVE INJURY

p 2017

EXPRESSION OF REGENERATION-ASSOCIATED PROTEINS IN THE PRIMARY SENSORY NEURONS AND REGENERATING AXONS AFTER NERVE INJURY

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Ivana HRADILOVÁ SVÍŽENSKÁ, Václav BRÁZDA, Marek JOUKAL et. al.

Základní údaje

Originální název

EXPRESSION OF REGENERATION-ASSOCIATED PROTEINS IN THE PRIMARY SENSORY NEURONS AND REGENERATING AXONS AFTER NERVE INJURY

Autoři

DUBOVÝ, Petr (203 Česká republika, garant, domácí), Ilona KLUSÁKOVÁ (203 Česká republika), Ivana HRADILOVÁ SVÍŽENSKÁ (203 Česká republika), Václav BRÁZDA (203 Česká republika) a Marek JOUKAL (203 Česká republika)

Vydání

2017

Další údaje

Jazyk

angličtina

Typ výsledku

Vyžádané přednášky

Obor

30103 Neurosciences

Stát vydavatele

Španělsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14110/17:00101403

Organizační jednotka

Lékařská fakulta

Klíčová slova česky

regenerační program; neurony; spinální ganglia; GAP43; SCG10; STAT3

Klíčová slova anglicky

regeneration program; neurons; spinal ganglia; GAP43; SCG10; STAT3

Příznaky

Mezinárodní význam

Změněno: 5. 12. 2018 12:02, prof. RNDr. Petr Dubový, CSc.

Anotace

V originále

EXPRESSION OF REGENERATION-ASSOCIATED PROTEINS IN THE PRIMARY SENSORY NEURONS AND REGENERATING AXONS AFTER NERVE INJURY Dubový P., Klusáková I., Hradilová-Svíženská I., Brázda V., Joukal M. Department of Anatomy, Cellular and Molecular Research Group, Faculty of Medicine, Masaryk University, Brno, Czech Republic Peripheral nerve injury results in profound alterations of affected neurons involving an interplay between intrinsic and extrinsic molecular events. Restart of regenerative neuronal program is important prerequisite for functional recovery of injured peripheral nerve. The primary sensory neurons with their cell bodies in the dorsal root ganglia (DRG) provide a useful in vivo model to study regulation of neuronal intrinsic regeneration capacity after axotomy. The aim of present experiments was to study quantitative immunodetection of GAP43 and SCG10 as markers of initiation of regeneration program in the rat primary sensory neurons and indicators of axon regeneration in the injured peripheral nerves. We found bilateral increase of GAP43 and SCG10 proteins in different size types of neuronal bodies after unilateral sciatic nerve injury. In addition, DRG sections were double immunostained for GAP43 and SCG10 as well as for the regeneration-associated proteins and molecular markers of neuronal subpopulations (NF200, CGRP, IB4) and activated STAT3. Double immunostaining of GAP43 and SCG10 revealed such differences indicating SCG10 as a better marker of regenerative status of the primary sensory neurons. The better results of regenerated axon detection one day after nerve crush were also obtained using SCG10 than GAP43 immunostaining. Supported by grant 16-08508S of The Czech Science Foundation.

Návaznosti

GA16-08508S, projekt VaV

Název: Zvýšení endogenního regeneračního programu a jeho aktivace zprostředkovaná cytokiny/chemokiny v neuronech ganglií bez spojení s poškozeným nervem (Akronym: ERP)

Investor: Grantová agentura ČR, Zvýšení endogenního regeneračního programu a jeho aktivace zprostředkovaná cytokiny/chemokiny

Citovat

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Ivana HRADILOVÁ SVÍŽENSKÁ, Václav BRÁZDA a Marek JOUKAL. EXPRESSION OF REGENERATION-ASSOCIATED PROTEINS IN THE PRIMARY SENSORY NEURONS AND REGENERATING AXONS AFTER NERVE INJURY. 2017.

@misc{1475196,
   author = {Dubový, Petr and Klusáková, Ilona and Hradilová Svíženská, Ivana and Brázda, Václav and Joukal, Marek},
   keywords = {regeneration program; neurons; spinal ganglia; GAP43; SCG10; STAT3},
   language = {eng},
   title = {EXPRESSION OF REGENERATION-ASSOCIATED PROTEINS IN THE PRIMARY SENSORY NEURONS AND REGENERATING AXONS AFTER NERVE INJURY},
   url = {https://sites.google.com/site/ispnr2017/program/full-programm},
   year = {2017}
}

TY  - SLIDE
ID  - 1475196
AU  - Dubový, Petr - Klusáková, Ilona - Hradilová Svíženská, Ivana - Brázda, Václav - Joukal, Marek
PY  - 2017
TI  - EXPRESSION OF REGENERATION-ASSOCIATED PROTEINS IN THE PRIMARY SENSORY NEURONS AND REGENERATING AXONS AFTER NERVE INJURY
KW  - regeneration program
KW  - neurons
KW  - spinal ganglia
KW  - GAP43
KW  - SCG10
KW  - STAT3
UR  - https://sites.google.com/site/ispnr2017/program/full-programm
L2  - https://sites.google.com/site/ispnr2017/program/full-programm
N2  - EXPRESSION OF REGENERATION-ASSOCIATED PROTEINS IN THE PRIMARY SENSORY NEURONS AND REGENERATING AXONS AFTER NERVE INJURY Dubový P., Klusáková I., Hradilová-Svíženská I., Brázda V., Joukal M. Department of Anatomy, Cellular and Molecular Research Group, Faculty of Medicine, Masaryk University, Brno, Czech Republic Peripheral nerve injury results in profound alterations of affected neurons involving an interplay between intrinsic and extrinsic molecular events. Restart of regenerative neuronal program is important prerequisite for functional recovery of injured peripheral nerve. The primary sensory neurons with their cell bodies in the dorsal root ganglia (DRG) provide a useful in vivo model to study regulation of neuronal intrinsic regeneration capacity after axotomy. The aim of present experiments was to study quantitative immunodetection of GAP43 and SCG10 as markers of initiation of regeneration program in the rat primary sensory neurons and indicators of axon regeneration in the injured peripheral nerves. We found bilateral increase of GAP43 and SCG10 proteins in different size types of neuronal bodies after unilateral sciatic nerve injury. In addition, DRG sections were double immunostained for GAP43 and SCG10 as well as for the regeneration-associated proteins and molecular markers of neuronal subpopulations (NF200, CGRP, IB4) and activated STAT3. Double immunostaining of GAP43 and SCG10 revealed such differences indicating SCG10 as a better marker of regenerative status of the primary sensory neurons. The better results of regenerated axon detection one day after nerve crush were also obtained using SCG10 than GAP43 immunostaining. Supported by grant 16-08508S of The Czech Science Foundation.
ER  -

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Ivana HRADILOVÁ SVÍŽENSKÁ, Václav BRÁZDA a Marek JOUKAL. \textit{EXPRESSION OF REGENERATION-ASSOCIATED PROTEINS IN THE PRIMARY SENSORY NEURONS AND REGENERATING AXONS AFTER NERVE INJURY}. 2017.

Podrobný výpis o publikaci