Dynamics of the Pollen Sequestrome Defined by Subcellular
Coupled Omics

J 2018

Dynamics of the Pollen Sequestrome Defined by Subcellular Coupled Omics

HAFIDH, Said, David POTĚŠIL, Karel MULLER, Jan FÍLA, Christos MICHAILIDIS et. al.

Základní údaje

Originální název

Dynamics of the Pollen Sequestrome Defined by Subcellular Coupled Omics

Autoři

HAFIDH, Said (203 Česká republika), David POTĚŠIL (203 Česká republika, domácí), Karel MULLER (203 Česká republika), Jan FÍLA (203 Česká republika), Christos MICHAILIDIS (203 Česká republika), Anna HERRMANNOVÁ (203 Česká republika), Jana FECÍKOVÁ (203 Česká republika), Till ISCHEBECK (276 Německo), Leoš Shivaya VALÁŠEK (203 Česká republika), Zbyněk ZDRÁHAL (203 Česká republika, garant, domácí) a David HONYS

Vydání

Plant Physiology, American Society of Plant Physiologists, 2018, 0032-0889

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 6.305

Kód RIV

RIV/00216224:14740/18:00101408

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1104/pp.18.00648

UT WoS

000444004100022

Klíčová slova anglicky

DIFFERENTIALLY EXPRESSED PROTEINS; MALE GAMETOPHYTE DEVELOPMENT; MESSENGER-RNA TRANSPORT; TUBE GROWTH; NICOTIANA-TABACUM; GENE-EXPRESSION; ARABIDOPSIS-THALIANA; MATURE POLLEN; PROTEOMIC ANALYSIS; PROCESSING BODIES

Štítky

CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 3. 2019 11:15, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Reproduction success in angiosperm plants depends on robust pollen tube growth through the female pistil tissues to ensure successful fertilization. Accordingly, there is an apparent evolutionary trend to accumulate significant reserves during pollen maturation, including a population of stored mRNAs, that are utilized later for a massive translation of various proteins in growing pollen tubes. Here, we performed a thorough transcriptomic and proteomic analysis of stored and translated transcripts in three subcellular compartments of tobacco (Nicotiana tabacum), long-term storage EDTA/puromycin-resistant particles, translating polysomes, and free ribonuclear particles, throughout tobacco pollen development and in in vitro-growing pollen tubes. We demonstrated that the composition of the aforementioned complexes is not rigid and that numerous transcripts were redistributed among these complexes during pollen development, which may represent an important mechanism of translational regulation. Therefore, we defined the pollen sequestrome as a distinct and highly dynamic compartment for the storage of stable, translationally repressed transcripts and demonstrated its dynamics. We propose that EDTA/puromycin-resistant particle complexes represent aggregated nontranslating monosomes as the primary mediators of messenger RNA sequestration. Such organization is extremely useful in fast tip-growing pollen tubes, where rapid and orchestrated protein synthesis must take place in specific regions.

Návaznosti

GA18-02448S, projekt VaV

Název: Úloha translačních iniciačních faktorů při skladování a aktivaci transkriptů v samčí zárodečné linii krytosemenných rostlin

Investor: Grantová agentura ČR, Úloha translačních iniciačních faktorů při skladování a aktivaci transkriptů v samčí zárodečné linii krytosemenných rostlin

LM2015043, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LM2015085, projekt VaV

Název: CERIT Scientific Cloud (Akronym: CERIT-SC)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CERIT Scientific Cloud

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

HAFIDH, Said, David POTĚŠIL, Karel MULLER, Jan FÍLA, Christos MICHAILIDIS, Anna HERRMANNOVÁ, Jana FECÍKOVÁ, Till ISCHEBECK, Leoš Shivaya VALÁŠEK, Zbyněk ZDRÁHAL a David HONYS. Dynamics of the Pollen Sequestrome Defined by Subcellular Coupled Omics. Plant Physiology. American Society of Plant Physiologists, 2018, roč. 178, č. 1, s. 258-282. ISSN 0032-0889. Dostupné z: https://dx.doi.org/10.1104/pp.18.00648.

@article{1475805,
   author = {Hafidh, Said and Potěšil, David and Muller, Karel and Fíla, Jan and Michailidis, Christos and Herrmannová, Anna and Fecíková, Jana and Ischebeck, Till and Valášek, Leoš Shivaya and Zdráhal, Zbyněk and Honys, David},
   article_number = {1},
   doi = {http://dx.doi.org/10.1104/pp.18.00648},
   keywords = {DIFFERENTIALLY EXPRESSED PROTEINS; MALE GAMETOPHYTE DEVELOPMENT; MESSENGER-RNA TRANSPORT; TUBE GROWTH; NICOTIANA-TABACUM; GENE-EXPRESSION; ARABIDOPSIS-THALIANA; MATURE POLLEN; PROTEOMIC ANALYSIS; PROCESSING BODIES},
   language = {eng},
   issn = {0032-0889},
   journal = {Plant Physiology},
   title = {Dynamics of the Pollen Sequestrome Defined by Subcellular Coupled Omics},
   volume = {178},
   year = {2018}
}

TY  - JOUR
ID  - 1475805
AU  - Hafidh, Said - Potěšil, David - Muller, Karel - Fíla, Jan - Michailidis, Christos - Herrmannová, Anna - Fecíková, Jana - Ischebeck, Till - Valášek, Leoš Shivaya - Zdráhal, Zbyněk - Honys, David
PY  - 2018
TI  - Dynamics of the Pollen Sequestrome Defined by Subcellular Coupled Omics
JF  - Plant Physiology
VL  - 178
IS  - 1
SP  - 258-282
EP  - 258-282
PB  - American Society of Plant Physiologists
SN  - 00320889
KW  - DIFFERENTIALLY EXPRESSED PROTEINS
KW  - MALE GAMETOPHYTE DEVELOPMENT
KW  - MESSENGER-RNA TRANSPORT
KW  - TUBE GROWTH
KW  - NICOTIANA-TABACUM
KW  - GENE-EXPRESSION
KW  - ARABIDOPSIS-THALIANA
KW  - MATURE POLLEN
KW  - PROTEOMIC ANALYSIS
KW  - PROCESSING BODIES
N2  - Reproduction success in angiosperm plants depends on robust pollen tube growth through the female pistil tissues to ensure successful fertilization. Accordingly, there is an apparent evolutionary trend to accumulate significant reserves during pollen maturation, including a population of stored mRNAs, that are utilized later for a massive translation of various proteins in growing pollen tubes. Here, we performed a thorough transcriptomic and proteomic analysis of stored and translated transcripts in three subcellular compartments of tobacco (Nicotiana tabacum), long-term storage EDTA/puromycin-resistant particles, translating polysomes, and free ribonuclear particles, throughout tobacco pollen development and in in vitro-growing pollen tubes. We demonstrated that the composition of the aforementioned complexes is not rigid and that numerous transcripts were redistributed among these complexes during pollen development, which may represent an important mechanism of translational regulation. Therefore, we defined the pollen sequestrome as a distinct and highly dynamic compartment for the storage of stable, translationally repressed transcripts and demonstrated its dynamics. We propose that EDTA/puromycin-resistant particle complexes represent aggregated nontranslating monosomes as the primary mediators of messenger RNA sequestration. Such organization is extremely useful in fast tip-growing pollen tubes, where rapid and orchestrated protein synthesis must take place in specific regions.
ER  -

HAFIDH, Said, David POTĚŠIL, Karel MULLER, Jan FÍLA, Christos MICHAILIDIS, Anna HERRMANNOVÁ, Jana FECÍKOVÁ, Till ISCHEBECK, Leoš Shivaya VALÁŠEK, Zbyněk ZDRÁHAL a David HONYS. Dynamics of the Pollen Sequestrome Defined by Subcellular Coupled Omics. \textit{Plant Physiology}. American Society of Plant Physiologists, 2018, roč.~178, č.~1, s.~258-282. ISSN~0032-0889. Dostupné z: https://dx.doi.org/10.1104/pp.18.00648.

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