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@article{1477346,
author = {Šedo, Ondrej and RadolfováandKřížová, Lenka and Nemec, Alexandr and Zdráhal, Zbyněk},
article_number = {NOV 2018},
doi = {http://dx.doi.org/10.1016/j.mimet.2018.10.009},
keywords = {Acinetobacter spp.; Bacteria profiling; BioTyper; MALDI-TOF MS},
language = {eng},
issn = {0167-7012},
journal = {Journal of Microbiological Methods},
title = {Limitations of routine MALDI-TOF mass spectrometric identification of Acinetobacter species and remedial actions},
volume = {154},
year = {2018}
}
TY - JOUR
ID - 1477346
AU - Šedo, Ondrej - Radolfová-Křížová, Lenka - Nemec, Alexandr - Zdráhal, Zbyněk
PY - 2018
TI - Limitations of routine MALDI-TOF mass spectrometric identification of Acinetobacter species and remedial actions
JF - Journal of Microbiological Methods
VL - 154
IS - NOV 2018
SP - 79-85
EP - 79-85
PB - Elsevier
SN - 01677012
KW - Acinetobacter spp.
KW - Bacteria profiling
KW - BioTyper
KW - MALDI-TOF MS
N2 - A set of 204 taxonomically well-defined strains belonging to 17 Acinetobacter spp., including 11 recently described species (A. albensis, A. bohemicus, A. colistiniresistens, A. courvalinii, A. dispersus, A. gandensis, A. modestus, A. proteolyticus, A. seifertii, A. variabilis, and A. vivianii) and six species of the so-called haemolytic Glade (A. beijerinckii, A. gyllenbergii, A. haemolyticus, A. junii, A. parvus, and A. venetianus), were subjected to MALDI-TOF mass spectrometric profiling. The identification outputs were evaluated using the current version (8.0.0.0) of the commercially available Bruker Daltonics, Biotyper database, which does not contain reference entries for six of the species tested. Up to 29% of the strains were falsely identified as different Acinetobacter spp. present in the Biotyper database, resulting mostly from the close phylogenetic relationship of species of the haemolytic Glade. To obtain more reliable identification, extending the commercial database showed only partial improvement, while the use of an alternative MALDI matrix solution (strongly acidified ferulic acid) allowed correct identification of nearly all problematic strains.
ER -
ŠEDO, Ondrej, Lenka RADOLFOVÁ-KŘÍŽOVÁ, Alexandr NEMEC a Zbyněk ZDRÁHAL. Limitations of routine MALDI-TOF mass spectrometric identification of Acinetobacter species and remedial actions. \textit{Journal of Microbiological Methods}. Elsevier, 2018, roč.~154, NOV 2018, s.~79-85. ISSN~0167-7012. Dostupné z: https://dx.doi.org/10.1016/j.mimet.2018.10.009.
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