Limitations of routine MALDI-TOF mass spectrometric
identification of Acinetobacter species and remedial actions

J 2018

Limitations of routine MALDI-TOF mass spectrometric identification of Acinetobacter species and remedial actions

ŠEDO, Ondrej, Lenka RADOLFOVÁ-KŘÍŽOVÁ, Alexandr NEMEC a Zbyněk ZDRÁHAL

Základní údaje

Originální název

Limitations of routine MALDI-TOF mass spectrometric identification of Acinetobacter species and remedial actions

Autoři

ŠEDO, Ondrej (203 Česká republika, domácí), Lenka RADOLFOVÁ-KŘÍŽOVÁ, Alexandr NEMEC a Zbyněk ZDRÁHAL (203 Česká republika, garant, domácí)

Vydání

Journal of Microbiological Methods, Elsevier, 2018, 0167-7012

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.803

Kód RIV

RIV/00216224:14740/18:00101425

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.mimet.2018.10.009

UT WoS

000451490900013

Klíčová slova anglicky

Acinetobacter spp.; Bacteria profiling; BioTyper; MALDI-TOF MS

Štítky

CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 3. 2019 16:16, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

A set of 204 taxonomically well-defined strains belonging to 17 Acinetobacter spp., including 11 recently described species (A. albensis, A. bohemicus, A. colistiniresistens, A. courvalinii, A. dispersus, A. gandensis, A. modestus, A. proteolyticus, A. seifertii, A. variabilis, and A. vivianii) and six species of the so-called haemolytic Glade (A. beijerinckii, A. gyllenbergii, A. haemolyticus, A. junii, A. parvus, and A. venetianus), were subjected to MALDI-TOF mass spectrometric profiling. The identification outputs were evaluated using the current version (8.0.0.0) of the commercially available Bruker Daltonics, Biotyper database, which does not contain reference entries for six of the species tested. Up to 29% of the strains were falsely identified as different Acinetobacter spp. present in the Biotyper database, resulting mostly from the close phylogenetic relationship of species of the haemolytic Glade. To obtain more reliable identification, extending the commercial database showed only partial improvement, while the use of an alternative MALDI matrix solution (strongly acidified ferulic acid) allowed correct identification of nearly all problematic strains.

Návaznosti

EF16_013/0001776, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii pro lidské zdraví

GBP206/12/G151, projekt VaV

Název: Centrum nových přístupů k bioanalýze a molekulární diagnostice

LM2015043, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

ŠEDO, Ondrej, Lenka RADOLFOVÁ-KŘÍŽOVÁ, Alexandr NEMEC a Zbyněk ZDRÁHAL. Limitations of routine MALDI-TOF mass spectrometric identification of Acinetobacter species and remedial actions. Journal of Microbiological Methods. Elsevier, 2018, roč. 154, NOV 2018, s. 79-85. ISSN 0167-7012. Dostupné z: https://dx.doi.org/10.1016/j.mimet.2018.10.009.

@article{1477346,
   author = {Šedo, Ondrej and RadolfováandKřížová, Lenka and Nemec, Alexandr and Zdráhal, Zbyněk},
   article_number = {NOV 2018},
   doi = {http://dx.doi.org/10.1016/j.mimet.2018.10.009},
   keywords = {Acinetobacter spp.; Bacteria profiling; BioTyper; MALDI-TOF MS},
   language = {eng},
   issn = {0167-7012},
   journal = {Journal of Microbiological Methods},
   title = {Limitations of routine MALDI-TOF mass spectrometric identification of Acinetobacter species and remedial actions},
   volume = {154},
   year = {2018}
}

TY  - JOUR
ID  - 1477346
AU  - Šedo, Ondrej - Radolfová-Křížová, Lenka - Nemec, Alexandr - Zdráhal, Zbyněk
PY  - 2018
TI  - Limitations of routine MALDI-TOF mass spectrometric identification of Acinetobacter species and remedial actions
JF  - Journal of Microbiological Methods
VL  - 154
IS  - NOV 2018
SP  - 79-85
EP  - 79-85
PB  - Elsevier
SN  - 01677012
KW  - Acinetobacter spp.
KW  - Bacteria profiling
KW  - BioTyper
KW  - MALDI-TOF MS
N2  - A set of 204 taxonomically well-defined strains belonging to 17 Acinetobacter spp., including 11 recently described species (A. albensis, A. bohemicus, A. colistiniresistens, A. courvalinii, A. dispersus, A. gandensis, A. modestus, A. proteolyticus, A. seifertii, A. variabilis, and A. vivianii) and six species of the so-called haemolytic Glade (A. beijerinckii, A. gyllenbergii, A. haemolyticus, A. junii, A. parvus, and A. venetianus), were subjected to MALDI-TOF mass spectrometric profiling. The identification outputs were evaluated using the current version (8.0.0.0) of the commercially available Bruker Daltonics, Biotyper database, which does not contain reference entries for six of the species tested. Up to 29% of the strains were falsely identified as different Acinetobacter spp. present in the Biotyper database, resulting mostly from the close phylogenetic relationship of species of the haemolytic Glade. To obtain more reliable identification, extending the commercial database showed only partial improvement, while the use of an alternative MALDI matrix solution (strongly acidified ferulic acid) allowed correct identification of nearly all problematic strains.
ER  -

ŠEDO, Ondrej, Lenka RADOLFOVÁ-KŘÍŽOVÁ, Alexandr NEMEC a Zbyněk ZDRÁHAL. Limitations of routine MALDI-TOF mass spectrometric identification of Acinetobacter species and remedial actions. \textit{Journal of Microbiological Methods}. Elsevier, 2018, roč.~154, NOV 2018, s.~79-85. ISSN~0167-7012. Dostupné z: https://dx.doi.org/10.1016/j.mimet.2018.10.009.

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