Online Enantioselective Capillary Electrophoretic Method for
Screening Cytochrome P450 3A4 Inhibitors

C 2020

Online Enantioselective Capillary Electrophoretic Method for Screening Cytochrome P450 3A4 Inhibitors

ŘEMÍNEK, Roman, Zdeněk GLATZ a Wolfgang THORMANN

Základní údaje

Originální název

Online Enantioselective Capillary Electrophoretic Method for Screening Cytochrome P450 3A4 Inhibitors

Název česky

Online enantioselektivní kapilárně elektroforetická metoda pro screening inhibitorů cytochromu P450 3A4

Autoři

ŘEMÍNEK, Roman (203 Česká republika, domácí), Zdeněk GLATZ (203 Česká republika, garant, domácí) a Wolfgang THORMANN (756 Švýcarsko)

Vydání

1. vyd. USA, Targeting Enzymes for Pharmaceutical Development: Methods and Protocols, od s. 167-178, 12 s. Targeting Enzymes for Pharmaceutical Development, 2020

Nakladatel

Springer

Další údaje

Jazyk

angličtina

Typ výsledku

Kapitola resp. kapitoly v odborné knize

Obor

10609 Biochemical research methods

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Odkazy

URL

Kód RIV

RIV/00216224:14310/20:00113958

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-1-0716-0162-4

DOI

http://dx.doi.org/10.1007/978-1-0716-0163-1_11

UT WoS

000650966200012

Klíčová slova česky

Kapilární elektroforéza; Enantioselektivní separace; Cytochrom P450; CYP3A4; Ketamin

Klíčová slova anglicky

Capillary electrophoresis; Enantioselective separation; Cytochrome P450; CYP3A4; Ketamine

Štítky

rivok, topvydavatel

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 8. 2021 12:46, Mgr. Marie Šípková, DiS.

Anotace

ORIG CZ

V originále

The market share of single-enantiomer drugs is steadily increasing. The pharmacodynamics and pharmacokinetics of individual enantiomers can differ considerably. Thus, their characteristics have to be addressed as early as possible in the development process of new pharmaceuticals. Capillary electrophoresis is a promising technique for enantioselective drug metabolism studies due to highly effective separations, minuscule consumption of sample and reagents, compatibility with a variety of detection techniques, high-throughput via automation, and the implementation of on-line procedures. An on-line method comprised of the diffusion-based mixing of cytochrome P450 3A4 with racemic ketamine, incubation of the enzyme reaction, separation of the reaction products S- and R-norketamine and their quantification is presented in this chapter. Since diffusion is an inherent property of all molecules, the method enables the addition of virtually any compound to the reaction mixture without the need for additional optimization of the mixing conditions, and thus can be favorably used for the rapid screening of putative cytochrome P450 3A4 inhibitors.

Česky

Byl publikován protokol pro screening inhibitorů cytochromu P450 3A4 pomocí on-line enantioselektivní metody založené na kapilární elektroforéze.

Návaznosti

GA16-06106S, projekt VaV

Název: Vysoce efektivní systém založený na kapilární elektroforéze pro screening inhibitorů beta-sekretázy jako terapeutického cíle pro Alzheimerovu chorobu (Akronym: Alzheimer)

Investor: Grantová agentura ČR, Vysoce efektivní systém založený na kapilární elektroforéze pro screening inhibitorů beta-sekretázy jako terapeutického cíle pro Alzheimerovu chorobu

Citovat

ŘEMÍNEK, Roman, Zdeněk GLATZ a Wolfgang THORMANN. Online Enantioselective Capillary Electrophoretic Method for Screening Cytochrome P450 3A4 Inhibitors. In N.E. Labrou. Targeting Enzymes for Pharmaceutical Development: Methods and Protocols. 1. vyd. USA: Springer, 2020, s. 167-178. Targeting Enzymes for Pharmaceutical Development. ISBN 978-1-0716-0162-4. Dostupné z: https://dx.doi.org/10.1007/978-1-0716-0163-1_11.

@inbook{1482480,
   author = {Řemínek, Roman and Glatz, Zdeněk and Thormann, Wolfgang},
   address = {USA},
   booktitle = {Targeting Enzymes for Pharmaceutical Development: Methods and Protocols},
   doi = {http://dx.doi.org/10.1007/978-1-0716-0163-1_11},
   edition = {1.},
   editor = {N.E. Labrou},
   keywords = {Capillary electrophoresis; Enantioselective separation; Cytochrome P450; CYP3A4; Ketamine},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {USA},
   isbn = {978-1-0716-0162-4},
   pages = {167-178},
   publisher = {Springer},
   title = {Online Enantioselective Capillary Electrophoretic Method for Screening Cytochrome P450 3A4 Inhibitors},
   url = {https://link.springer.com/protocol/10.1007%2F978-1-0716-0163-1_11},
   year = {2020}
}

TY  - CHAP
ID  - 1482480
AU  - Řemínek, Roman - Glatz, Zdeněk - Thormann, Wolfgang
PY  - 2020
TI  - Online Enantioselective Capillary Electrophoretic Method for Screening Cytochrome P450 3A4 Inhibitors
VL  - Targeting Enzymes for Pharmaceutical Development
PB  - Springer
CY  - USA
SN  - 9781071601624
KW  - Capillary electrophoresis
KW  - Enantioselective separation
KW  - Cytochrome P450
KW  - CYP3A4
KW  - Ketamine
UR  - https://link.springer.com/protocol/10.1007%2F978-1-0716-0163-1_11
N2  - The market share of single-enantiomer drugs is steadily increasing. The pharmacodynamics and pharmacokinetics of individual enantiomers can differ considerably. Thus, their characteristics have to be addressed as early as possible in the development process of new pharmaceuticals. Capillary electrophoresis is a promising technique for enantioselective drug metabolism studies due to highly effective separations, minuscule consumption of sample and reagents, compatibility with a variety of detection techniques, high-throughput via automation, and the implementation of on-line procedures. An on-line method comprised of the diffusion-based mixing of cytochrome P450 3A4 with racemic ketamine, incubation of the enzyme reaction, separation of the reaction products S- and R-norketamine and their quantification is presented in this chapter. Since diffusion is an inherent property of all molecules, the method enables the addition of virtually any compound to the reaction mixture without the need for additional optimization of the mixing conditions, and thus can be favorably used for the rapid screening of putative cytochrome P450 3A4 inhibitors.
ER  -

ŘEMÍNEK, Roman, Zdeněk GLATZ a Wolfgang THORMANN. Online Enantioselective Capillary Electrophoretic Method for Screening Cytochrome P450 3A4 Inhibitors. In N.E. Labrou. \textit{Targeting Enzymes for Pharmaceutical Development: Methods and Protocols}. 1. vyd. USA: Springer, 2020, s.~167-178. Targeting Enzymes for Pharmaceutical Development. ISBN~978-1-0716-0162-4. Dostupné z: https://dx.doi.org/10.1007/978-1-0716-0163-1\_{}11.

Podrobný výpis o publikaci