Preparative and capillary isoelectric focusing for detection
and identification of Aspergillus conidia in complex sample
matrices

J 2018

Preparative and capillary isoelectric focusing for detection and identification of Aspergillus conidia in complex sample matrices

HORKÁ, Marie, Jiří ŠALPLACHTA, Filip RŮŽIČKA a Karel ŠLAIS

Základní údaje

Originální název

Preparative and capillary isoelectric focusing for detection and identification of Aspergillus conidia in complex sample matrices

Autoři

HORKÁ, Marie (203 Česká republika, garant), Jiří ŠALPLACHTA (203 Česká republika), Filip RŮŽIČKA (203 Česká republika, domácí) a Karel ŠLAIS (203 Česká republika)

Vydání

Journal of Separation Science, Germany, WILEY-V C H VERLAG GMBH, 2018, 1615-9306

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.516

Kód RIV

RIV/00216224:14110/18:00106898

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1002/jssc.201800547

UT WoS

000450408300013

Klíčová slova anglicky

capillary isoelectric focusing; peanuts; red pepper powder

Štítky

14110113, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 3. 2019 12:29, Soňa Böhmová

Anotace

V originále

This study describes a new method for fast identification of highly hydrophobic conidia of Aspergillus species from both simple and complex matrices. The method is based on recently developed preparative isoelectric focusing in a cellulose-based separation medium which had to be modified with respect to the highly hydrophobic surface of the conidia. Although Aspergillus conidia are colored, their zones in the cellulose bed were indicated by colored isoelectric point markers. The isoelectric point values of Aspergillus conidia were determined by capillary isoelectric focusing. Preparative isoelectric focusing was successfully used for preconcentration of individual conidia of cultivated strains of Aspergillus niger, Aspergillus fumigatus, Aspergillus flavus, and Aspergillus parasiticus, and also for separation of the conidia in a mixture. Subsequently, red pepper powder and peanuts spiked with Aspergillus niger and Aspergillus flavus conidia, respectively, were used as complex matrices. The detection limit for identification of the conidia in these complex matrices is 10(4) conidia mL(-1). The presence of conidia in the focused zones was confirmed by their subsequent analysis by capillary isoelectric focusing. Their viability was confirmed by a cultivation of the conidia extracted from the collected fractions after preparative isoelectric focusing.

Návaznosti

NV16-29916A, projekt VaV

Název: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu

VI20172020069, projekt VaV

Název: Vývoj moderních instrumentálních metod pro rychlou detekci a identifikaci vybraných B-agens a toxinů (Akronym: VMIMRDIBT)

Investor: Ministerstvo vnitra ČR, Vývoj moderních instrumentálních metod pro rychlou detekci a identifikaci vybraných B-agens a toxinů

Citovat

HORKÁ, Marie, Jiří ŠALPLACHTA, Filip RŮŽIČKA a Karel ŠLAIS. Preparative and capillary isoelectric focusing for detection and identification of Aspergillus conidia in complex sample matrices. Journal of Separation Science. Germany: WILEY-V C H VERLAG GMBH, 2018, roč. 41, č. 22, s. 1-10. ISSN 1615-9306. Dostupné z: https://dx.doi.org/10.1002/jssc.201800547.

@article{1482642,
   author = {Horká, Marie and Šalplachta, Jiří and Růžička, Filip and Šlais, Karel},
   article_location = {Germany},
   article_number = {22},
   doi = {http://dx.doi.org/10.1002/jssc.201800547},
   keywords = {capillary isoelectric focusing; peanuts; red pepper powder},
   language = {eng},
   issn = {1615-9306},
   journal = {Journal of Separation Science},
   title = {Preparative and capillary isoelectric focusing for detection and identification of Aspergillus conidia in complex sample matrices},
   volume = {41},
   year = {2018}
}

TY  - JOUR
ID  - 1482642
AU  - Horká, Marie - Šalplachta, Jiří - Růžička, Filip - Šlais, Karel
PY  - 2018
TI  - Preparative and capillary isoelectric focusing for detection and identification of Aspergillus conidia in complex sample matrices
JF  - Journal of Separation Science
VL  - 41
IS  - 22
SP  - 1-10
EP  - 1-10
PB  - WILEY-V C H VERLAG GMBH
SN  - 16159306
KW  - capillary isoelectric focusing
KW  - peanuts
KW  - red pepper powder
N2  - This study describes a new method for fast identification of highly hydrophobic conidia of Aspergillus species from both simple and complex matrices. The method is based on recently developed preparative isoelectric focusing in a cellulose-based separation medium which had to be modified with respect to the highly hydrophobic surface of the conidia. Although Aspergillus conidia are colored, their zones in the cellulose bed were indicated by colored isoelectric point markers. The isoelectric point values of Aspergillus conidia were determined by capillary isoelectric focusing. Preparative isoelectric focusing was successfully used for preconcentration of individual conidia of cultivated strains of Aspergillus niger, Aspergillus fumigatus, Aspergillus flavus, and Aspergillus parasiticus, and also for separation of the conidia in a mixture. Subsequently, red pepper powder and peanuts spiked with Aspergillus niger and Aspergillus flavus conidia, respectively, were used as complex matrices. The detection limit for identification of the conidia in these complex matrices is 10(4) conidia mL(-1). The presence of conidia in the focused zones was confirmed by their subsequent analysis by capillary isoelectric focusing. Their viability was confirmed by a cultivation of the conidia extracted from the collected fractions after preparative isoelectric focusing.
ER  -

HORKÁ, Marie, Jiří ŠALPLACHTA, Filip RŮŽIČKA a Karel ŠLAIS. Preparative and capillary isoelectric focusing for detection and identification of Aspergillus conidia in complex sample matrices. \textit{Journal of Separation Science}. Germany: WILEY-V C H VERLAG GMBH, 2018, roč.~41, č.~22, s.~1-10. ISSN~1615-9306. Dostupné z: https://dx.doi.org/10.1002/jssc.201800547.

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