Preparative and capillary isoelectric focusing for detection
and identification of Aspergillus conidia in complex sample
matrices

J 2018

Preparative and capillary isoelectric focusing for detection and identification of Aspergillus conidia in complex sample matrices

HORKÁ, Marie, Jiří ŠALPLACHTA, Filip RŮŽIČKA and Karel ŠLAIS

Basic information

Original name

Preparative and capillary isoelectric focusing for detection and identification of Aspergillus conidia in complex sample matrices

Authors

HORKÁ, Marie (203 Czech Republic, guarantor), Jiří ŠALPLACHTA (203 Czech Republic), Filip RŮŽIČKA (203 Czech Republic, belonging to the institution) and Karel ŠLAIS (203 Czech Republic)

Edition

Journal of Separation Science, Germany, WILEY-V C H VERLAG GMBH, 2018, 1615-9306

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.516

RIV identification code

RIV/00216224:14110/18:00106898

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1002/jssc.201800547

UT WoS

000450408300013

Keywords in English

capillary isoelectric focusing; peanuts; red pepper powder

Abstract

V originále

This study describes a new method for fast identification of highly hydrophobic conidia of Aspergillus species from both simple and complex matrices. The method is based on recently developed preparative isoelectric focusing in a cellulose-based separation medium which had to be modified with respect to the highly hydrophobic surface of the conidia. Although Aspergillus conidia are colored, their zones in the cellulose bed were indicated by colored isoelectric point markers. The isoelectric point values of Aspergillus conidia were determined by capillary isoelectric focusing. Preparative isoelectric focusing was successfully used for preconcentration of individual conidia of cultivated strains of Aspergillus niger, Aspergillus fumigatus, Aspergillus flavus, and Aspergillus parasiticus, and also for separation of the conidia in a mixture. Subsequently, red pepper powder and peanuts spiked with Aspergillus niger and Aspergillus flavus conidia, respectively, were used as complex matrices. The detection limit for identification of the conidia in these complex matrices is 10(4) conidia mL(-1). The presence of conidia in the focused zones was confirmed by their subsequent analysis by capillary isoelectric focusing. Their viability was confirmed by a cultivation of the conidia extracted from the collected fractions after preparative isoelectric focusing.

Links

NV16-29916A, research and development project

Name: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu

VI20172020069, research and development project

Name: Vývoj moderních instrumentálních metod pro rychlou detekci a identifikaci vybraných B-agens a toxinů (Acronym: VMIMRDIBT)

Investor: Ministry of the Interior of the CR

Citovat

HORKÁ, Marie, Jiří ŠALPLACHTA, Filip RŮŽIČKA and Karel ŠLAIS. Preparative and capillary isoelectric focusing for detection and identification of Aspergillus conidia in complex sample matrices. Journal of Separation Science. Germany: WILEY-V C H VERLAG GMBH, 2018, vol. 41, No 22, p. 1-10. ISSN 1615-9306. Available from: https://dx.doi.org/10.1002/jssc.201800547.

@article{1482642,
   author = {Horká, Marie and Šalplachta, Jiří and Růžička, Filip and Šlais, Karel},
   article_location = {Germany},
   article_number = {22},
   doi = {http://dx.doi.org/10.1002/jssc.201800547},
   keywords = {capillary isoelectric focusing; peanuts; red pepper powder},
   language = {eng},
   issn = {1615-9306},
   journal = {Journal of Separation Science},
   title = {Preparative and capillary isoelectric focusing for detection and identification of Aspergillus conidia in complex sample matrices},
   volume = {41},
   year = {2018}
}

TY  - JOUR
ID  - 1482642
AU  - Horká, Marie - Šalplachta, Jiří - Růžička, Filip - Šlais, Karel
PY  - 2018
TI  - Preparative and capillary isoelectric focusing for detection and identification of Aspergillus conidia in complex sample matrices
JF  - Journal of Separation Science
VL  - 41
IS  - 22
SP  - 1-10
EP  - 1-10
PB  - WILEY-V C H VERLAG GMBH
SN  - 16159306
KW  - capillary isoelectric focusing
KW  - peanuts
KW  - red pepper powder
N2  - This study describes a new method for fast identification of highly hydrophobic conidia of Aspergillus species from both simple and complex matrices. The method is based on recently developed preparative isoelectric focusing in a cellulose-based separation medium which had to be modified with respect to the highly hydrophobic surface of the conidia. Although Aspergillus conidia are colored, their zones in the cellulose bed were indicated by colored isoelectric point markers. The isoelectric point values of Aspergillus conidia were determined by capillary isoelectric focusing. Preparative isoelectric focusing was successfully used for preconcentration of individual conidia of cultivated strains of Aspergillus niger, Aspergillus fumigatus, Aspergillus flavus, and Aspergillus parasiticus, and also for separation of the conidia in a mixture. Subsequently, red pepper powder and peanuts spiked with Aspergillus niger and Aspergillus flavus conidia, respectively, were used as complex matrices. The detection limit for identification of the conidia in these complex matrices is 10(4) conidia mL(-1). The presence of conidia in the focused zones was confirmed by their subsequent analysis by capillary isoelectric focusing. Their viability was confirmed by a cultivation of the conidia extracted from the collected fractions after preparative isoelectric focusing.
ER  -

HORKÁ, Marie, Jiří ŠALPLACHTA, Filip RŮŽIČKA and Karel ŠLAIS. Preparative and capillary isoelectric focusing for detection and identification of Aspergillus conidia in complex sample matrices. \textit{Journal of Separation Science}. Germany: WILEY-V C H VERLAG GMBH, 2018, vol.~41, No~22, p.~1-10. ISSN~1615-9306. Available from: https://dx.doi.org/10.1002/jssc.201800547.

Detailed Information on Publication Record