The planar cell polarity protein VANG-1/Vangl negatively
regulates Wnt/beta-catenin signaling through a Dvl dependent
mechanism

J 2018

The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/beta-catenin signaling through a Dvl dependent mechanism

MENTINK, R.A., L. RELLA, Tomasz Witold RADASZKIEWICZ, Tomáš GYBEĽ, M.C. BETIST et. al.

Základní údaje

Originální název

The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/beta-catenin signaling through a Dvl dependent mechanism

Autoři

MENTINK, R.A. (528 Nizozemské království), L. RELLA (528 Nizozemské království), Tomasz Witold RADASZKIEWICZ (616 Polsko, domácí), Tomáš GYBEĽ (703 Slovensko, domácí), M.C. BETIST (528 Nizozemské království), Vítězslav BRYJA (203 Česká republika, garant, domácí) a H.C. KORSWAGEN (528 Nizozemské království)

Vydání

PLoS Genetics, San Francisco, Public Library of Science, 2018, 1553-7404

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.224

Kód RIV

RIV/00216224:14310/18:00101505

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1371/journal.pgen.1007840

UT WoS

000455099000023

Klíčová slova anglicky

CANONICAL WNT PATHWAY; C. ELEGANS; NEUROBLAST MIGRATION; GENE-EXPRESSION; VANGL2; GASTRULATION; MUTATIONS; DIVISION; AXIS; PHOSPHORYLATION

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 30. 4. 2019 09:34, Mgr. Tereza Miškechová

Anotace

V originále

Van Gogh-like (Vangl) and Prickle (Pk) are core components of the non-canonical Wnt planar cell polarity pathway that controls epithelial polarity and cell migration. Studies in vertebrate model systems have suggested that Vangl and Pk may also inhibit signaling through the canonical Wnt/beta-catenin pathway, but the functional significance of this potential crosstalk is unclear. In the nematode C. elegans, the Q neuroblasts and their descendants migrate in opposite directions along the anteroposterior body axis. The direction of these migrations is specified by Wnt signaling, with activation of canonical Wnt signaling driving posterior migration, and non-canonical Wnt signaling anterior migration. Here, we show that the Vangl ortholog VANG-1 influences the Wnt signaling response of the Q neuroblasts by negatively regulating canonical Wnt signaling. This inhibitory activity depends on a carboxyterminal PDZ binding motif in VANG-1 and the Dishevelled ortholog MIG-5, but is independent of the Pk ortholog PRKL-1. Moreover, using Vangl1 and Vangl2 double mutant cells, we show that a similar mechanism acts in mammalian cells. We conclude that cross-talk between VANG-1/Vangl and the canonical Wnt pathway is an evolutionarily conserved mechanism that ensures robust specification of Wnt signaling responses.

Návaznosti

GA17-16680S, projekt VaV

Název: Nové postupy pro určení aktivity dráhy planární buněčné polarity (PCP)

Investor: Grantová agentura ČR, Nové postupy pro určení aktivity dráhy planární buněčné polarity (PCP)

GA18-17658S, projekt VaV

Název: Odhalení tajemství signální dráhy WNT analýzou struktury proteinu Dishevelled

Investor: Grantová agentura ČR, Odhalení tajemství signální dráhy WNT analýzou struktury proteinu Dishevelled

Citovat

MENTINK, R.A., L. RELLA, Tomasz Witold RADASZKIEWICZ, Tomáš GYBEĽ, M.C. BETIST, Vítězslav BRYJA a H.C. KORSWAGEN. The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/beta-catenin signaling through a Dvl dependent mechanism. PLoS Genetics. San Francisco: Public Library of Science, 2018, roč. 14, č. 12, s. 1-22. ISSN 1553-7404. Dostupné z: https://dx.doi.org/10.1371/journal.pgen.1007840.

@article{1483381,
   author = {Mentink, R.A. and Rella, L. and Radaszkiewicz, Tomasz Witold and Gybeľ, Tomáš and Betist, M.C. and Bryja, Vítězslav and Korswagen, H.C.},
   article_location = {San Francisco},
   article_number = {12},
   doi = {http://dx.doi.org/10.1371/journal.pgen.1007840},
   keywords = {CANONICAL WNT PATHWAY; C. ELEGANS; NEUROBLAST MIGRATION; GENE-EXPRESSION; VANGL2; GASTRULATION; MUTATIONS; DIVISION; AXIS; PHOSPHORYLATION},
   language = {eng},
   issn = {1553-7404},
   journal = {PLoS Genetics},
   title = {The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/beta-catenin signaling through a Dvl dependent mechanism},
   url = {https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1007840},
   volume = {14},
   year = {2018}
}

TY  - JOUR
ID  - 1483381
AU  - Mentink, R.A. - Rella, L. - Radaszkiewicz, Tomasz Witold - Gybeľ, Tomáš - Betist, M.C. - Bryja, Vítězslav - Korswagen, H.C.
PY  - 2018
TI  - The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/beta-catenin signaling through a Dvl dependent mechanism
JF  - PLoS Genetics
VL  - 14
IS  - 12
SP  - 1-22
EP  - 1-22
PB  - Public Library of Science
SN  - 15537404
KW  - CANONICAL WNT PATHWAY
KW  - C. ELEGANS
KW  - NEUROBLAST MIGRATION
KW  - GENE-EXPRESSION
KW  - VANGL2
KW  - GASTRULATION
KW  - MUTATIONS
KW  - DIVISION
KW  - AXIS
KW  - PHOSPHORYLATION
UR  - https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1007840
L2  - https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1007840
N2  - Van Gogh-like (Vangl) and Prickle (Pk) are core components of the non-canonical Wnt planar cell polarity pathway that controls epithelial polarity and cell migration. Studies in vertebrate model systems have suggested that Vangl and Pk may also inhibit signaling through the canonical Wnt/beta-catenin pathway, but the functional significance of this potential crosstalk is unclear. In the nematode C. elegans, the Q neuroblasts and their descendants migrate in opposite directions along the anteroposterior body axis. The direction of these migrations is specified by Wnt signaling, with activation of canonical Wnt signaling driving posterior migration, and non-canonical Wnt signaling anterior migration. Here, we show that the Vangl ortholog VANG-1 influences the Wnt signaling response of the Q neuroblasts by negatively regulating canonical Wnt signaling. This inhibitory activity depends on a carboxyterminal PDZ binding motif in VANG-1 and the Dishevelled ortholog MIG-5, but is independent of the Pk ortholog PRKL-1. Moreover, using Vangl1 and Vangl2 double mutant cells, we show that a similar mechanism acts in mammalian cells. We conclude that cross-talk between VANG-1/Vangl and the canonical Wnt pathway is an evolutionarily conserved mechanism that ensures robust specification of Wnt signaling responses.
ER  -

MENTINK, R.A., L. RELLA, Tomasz Witold RADASZKIEWICZ, Tomáš GYBEĽ, M.C. BETIST, Vítězslav BRYJA a H.C. KORSWAGEN. The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/beta-catenin signaling through a Dvl dependent mechanism. \textit{PLoS Genetics}. San Francisco: Public Library of Science, 2018, roč.~14, č.~12, s.~1-22. ISSN~1553-7404. Dostupné z: https://dx.doi.org/10.1371/journal.pgen.1007840.

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