Capillary electrophoresis-based approaches for the study of
affinity interactions combined with various sensitive and
non-traditional detection techniques

J 2019

Capillary electrophoresis-based approaches for the study of affinity interactions combined with various sensitive and non-traditional detection techniques

NEVÍDALOVÁ, Hana, Lenka MICHALCOVÁ a Zdeněk GLATZ

Základní údaje

Originální název

Capillary electrophoresis-based approaches for the study of affinity interactions combined with various sensitive and non-traditional detection techniques

Název česky

Metody studia afinitních interakcí založené na CE v kombinaci s různými typy detekce

Autoři

NEVÍDALOVÁ, Hana (203 Česká republika, domácí), Lenka MICHALCOVÁ (203 Česká republika, domácí) a Zdeněk GLATZ (203 Česká republika, garant, domácí)

Vydání

Electrophoresis, Hoboken, Wiley, 2019, 0173-0835

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 3.081

Kód RIV

RIV/00216224:14310/19:00107222

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1002/elps.201800367

UT WoS

000460305100004

Klíčová slova česky

afinitní interakce; CE; LIF; MSů čipová CE.

Klíčová slova anglicky

Affinity interaction; capillary electrophoresis; fluorescence detection; mass spectrometry; microchip capillary electrophoresis.

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 12. 3. 2020 12:09, Mgr. Marie Šípková, DiS.

Anotace

V originále

Nearly all processes in living organisms are controlled and regulated by the synergy of many biomolecule interactions involving proteins, peptides, nucleic acids, nucleotides, saccharides and small molecular weight ligands. There is growing interest in understanding them, not only for the purposes of interactomics as an essential part of system biology, but also in their further elucidation in disease pathology, diagnostics and treatment. The necessity of detailed investigation of these interactions leads to the requirement of laboratory methods characterised by high efficiency and sensitivity. As a result, many instrumental approaches differing in their fundamental principles have been developed, including those based on capillary electrophoresis. Although capillary electrophoresis offers numerous advantages for such studies, it still has one serious limitation, its poor concentration sensitivity with the most commonly used detection method – ultraviolet-visible spectrometry. However, coupling capillary electrophoresis with a more sensitive detector fulfils the above-mentioned requirement. In this review, capillary electrophoresis combined with fluorescence, mass spectrometry and several non-traditional detection techniques in affinity interaction studies are summarised and discussed, together with the possibility of conducting these measurements in microchip format.

Návaznosti

GA16-06106S, projekt VaV

Název: Vysoce efektivní systém založený na kapilární elektroforéze pro screening inhibitorů beta-sekretázy jako terapeutického cíle pro Alzheimerovu chorobu (Akronym: Alzheimer)

Investor: Grantová agentura ČR, Vysoce efektivní systém založený na kapilární elektroforéze pro screening inhibitorů beta-sekretázy jako terapeutického cíle pro Alzheimerovu chorobu

GA19-08358S, projekt VaV

Název: Nové přístupy pro studium afinitních interakcí založené na kapilární elektroforéze

Investor: Grantová agentura ČR, Nove přístupy pro studium afinitních interakcí založené na kapilární elektroforéze

MUNI/A/1278/2016, interní kód MU

Název: Podpora biochemického výzkumu v roce 2017

Investor: Masarykova univerzita, Podpora biochemického výzkumu v roce 2017, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

NEVÍDALOVÁ, Hana, Lenka MICHALCOVÁ a Zdeněk GLATZ. Capillary electrophoresis-based approaches for the study of affinity interactions combined with various sensitive and non-traditional detection techniques. Electrophoresis. Hoboken: Wiley, 2019, roč. 40, č. 5, s. 625-642. ISSN 0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.201800367.

@article{1483892,
   author = {Nevídalová, Hana and Michalcová, Lenka and Glatz, Zdeněk},
   article_location = {Hoboken},
   article_number = {5},
   doi = {http://dx.doi.org/10.1002/elps.201800367},
   keywords = {Affinity interaction; capillary electrophoresis; fluorescence detection; mass spectrometry; microchip capillary electrophoresis.},
   language = {eng},
   issn = {0173-0835},
   journal = {Electrophoresis},
   title = {Capillary electrophoresis-based approaches for the study of affinity interactions combined with various sensitive and non-traditional detection techniques},
   url = {https://onlinelibrary.wiley.com/doi/full/10.1002/elps.201800367},
   volume = {40},
   year = {2019}
}

TY  - JOUR
ID  - 1483892
AU  - Nevídalová, Hana - Michalcová, Lenka - Glatz, Zdeněk
PY  - 2019
TI  - Capillary electrophoresis-based approaches for the study of affinity interactions combined with various sensitive and non-traditional detection techniques
JF  - Electrophoresis
VL  - 40
IS  - 5
SP  - 625-642
EP  - 625-642
PB  - Wiley
SN  - 01730835
KW  - Affinity interaction
KW  - capillary electrophoresis
KW  - fluorescence detection
KW  - mass spectrometry
KW  - microchip capillary electrophoresis.
UR  - https://onlinelibrary.wiley.com/doi/full/10.1002/elps.201800367
L2  - https://onlinelibrary.wiley.com/doi/full/10.1002/elps.201800367
N2  - Nearly all processes in living organisms are controlled and regulated by the synergy of many biomolecule interactions involving proteins, peptides, nucleic acids, nucleotides, saccharides and small molecular weight ligands. There is growing interest in understanding them, not only for the purposes of interactomics as an essential part of system biology, but also in their further elucidation in disease pathology, diagnostics and treatment. The necessity of detailed investigation of these interactions leads to the requirement of laboratory methods characterised by high efficiency and sensitivity. As a result, many instrumental approaches differing in their fundamental principles have been developed, including those based on capillary electrophoresis. Although capillary electrophoresis offers numerous advantages for such studies, it still has one serious limitation, its poor concentration sensitivity with the most commonly used detection method – ultraviolet-visible spectrometry. However, coupling capillary electrophoresis with a more sensitive detector fulfils the above-mentioned requirement. In this review, capillary electrophoresis combined with fluorescence, mass spectrometry and several non-traditional detection techniques in affinity interaction studies are summarised and discussed, together with the possibility of conducting these measurements in microchip format.
ER  -

NEVÍDALOVÁ, Hana, Lenka MICHALCOVÁ a Zdeněk GLATZ. Capillary electrophoresis-based approaches for the study of affinity interactions combined with various sensitive and non-traditional detection techniques. \textit{Electrophoresis}. Hoboken: Wiley, 2019, roč.~40, č.~5, s.~625-642. ISSN~0173-0835. Dostupné z: https://dx.doi.org/10.1002/elps.201800367.

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