Genome-wide identification of urinary cell-free microRNAs for
non-invasive detection of bladder cancer

J 2018

Genome-wide identification of urinary cell-free microRNAs for non-invasive detection of bladder cancer

JURÁČEK, Jaroslav, Barbora PELTANOVÁ, Jan DOLEŽEL, Michal FEDORKO, Dalibor PACÍK et. al.

Základní údaje

Originální název

Genome-wide identification of urinary cell-free microRNAs for non-invasive detection of bladder cancer

Autoři

JURÁČEK, Jaroslav (203 Česká republika, domácí), Barbora PELTANOVÁ (203 Česká republika, domácí), Jan DOLEŽEL (203 Česká republika), Michal FEDORKO (703 Slovensko, domácí), Dalibor PACÍK (203 Česká republika, domácí), Lenka RADOVÁ (203 Česká republika, domácí), Petra VESELA (203 Česká republika), Marek SVOBODA (203 Česká republika), Ondřej SLABÝ (203 Česká republika, garant, domácí) a Michal STANÍK (703 Slovensko, domácí)

Vydání

Journal of Cellular and Molecular Medicine, Hoboken, NJ USA, Wiley, 2018, 1582-4934

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.658

Kód RIV

RIV/00216224:14740/18:00105394

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1111/jcmm.13487

UT WoS

000426069300060

Klíčová slova anglicky

bladder cancer; cell-free miRNAs; urine; biomarker; non-invasive diagnosis

Štítky

14110225, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 3. 2019 13:23, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Urinary microRNAs (miRNAs) are emerging as clinically useful tool for early and non-invasive detection of various types of cancer including bladder cancer (BCA). In this study, 205 patients with BCA and 99 healthy controls were prospectively enrolled. Expression profiles of urinary miRNAs were obtained using Affymetrix miRNA microarrays (2578 miRNAs) and candidate miRNAs further validated in independent cohorts using qRT-PCR. Whole-genome profiling identified 76 miRNAs with significantly different concentrations in urine of BCA compared to controls (P < 0.01). In the training and independent validation phase of the study, miR-31-5p, miR-93-5p and miR-191-5p were confirmed to have significantly higher levels in urine of patients with BCA in comparison with controls (P < 0.01). We further established 2-miRNA-based urinary DxScore (miR-93-5p, miR-31-5p) enabling sensitive BCA detection with AUC being 0.84 and 0.81 in the training and validation phase, respectively. Moreover, DxScore significantly differed in the various histopathological subgroups of BCA and decreased post-operatively. In conclusion, we identified and independently validated cell-free urinary miRNAs as promising biomarkers enabling non-invasive detection of BCA.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

NV15-31071A, projekt VaV

Název: Studium močových/tkáňových mikroRNA jako potenciálních biomarkerů uroteliálního karcinomu močového měchýře

Citovat

JURÁČEK, Jaroslav, Barbora PELTANOVÁ, Jan DOLEŽEL, Michal FEDORKO, Dalibor PACÍK, Lenka RADOVÁ, Petra VESELA, Marek SVOBODA, Ondřej SLABÝ a Michal STANÍK. Genome-wide identification of urinary cell-free microRNAs for non-invasive detection of bladder cancer. Journal of Cellular and Molecular Medicine. Hoboken, NJ USA: Wiley, 2018, roč. 22, č. 3, s. 2033-2038. ISSN 1582-4934. Dostupné z: https://dx.doi.org/10.1111/jcmm.13487.

@article{1485296,
   author = {Juráček, Jaroslav and Peltanová, Barbora and Doležel, Jan and Fedorko, Michal and Pacík, Dalibor and Radová, Lenka and Vesela, Petra and Svoboda, Marek and Slabý, Ondřej and Staník, Michal},
   article_location = {Hoboken, NJ USA},
   article_number = {3},
   doi = {http://dx.doi.org/10.1111/jcmm.13487},
   keywords = {bladder cancer; cell-free miRNAs; urine; biomarker; non-invasive diagnosis},
   language = {eng},
   issn = {1582-4934},
   journal = {Journal of Cellular and Molecular Medicine},
   title = {Genome-wide identification of urinary cell-free microRNAs for non-invasive detection of bladder cancer},
   volume = {22},
   year = {2018}
}

TY  - JOUR
ID  - 1485296
AU  - Juráček, Jaroslav - Peltanová, Barbora - Doležel, Jan - Fedorko, Michal - Pacík, Dalibor - Radová, Lenka - Vesela, Petra - Svoboda, Marek - Slabý, Ondřej - Staník, Michal
PY  - 2018
TI  - Genome-wide identification of urinary cell-free microRNAs for non-invasive detection of bladder cancer
JF  - Journal of Cellular and Molecular Medicine
VL  - 22
IS  - 3
SP  - 2033-2038
EP  - 2033-2038
PB  - Wiley
SN  - 15824934
KW  - bladder cancer
KW  - cell-free miRNAs
KW  - urine
KW  - biomarker
KW  - non-invasive diagnosis
N2  - Urinary microRNAs (miRNAs) are emerging as clinically useful tool for early and non-invasive detection of various types of cancer including bladder cancer (BCA). In this study, 205 patients with BCA and 99 healthy controls were prospectively enrolled. Expression profiles of urinary miRNAs were obtained using Affymetrix miRNA microarrays (2578 miRNAs) and candidate miRNAs further validated in independent cohorts using qRT-PCR. Whole-genome profiling identified 76 miRNAs with significantly different concentrations in urine of BCA compared to controls (P < 0.01). In the training and independent validation phase of the study, miR-31-5p, miR-93-5p and miR-191-5p were confirmed to have significantly higher levels in urine of patients with BCA in comparison with controls (P < 0.01). We further established 2-miRNA-based urinary DxScore (miR-93-5p, miR-31-5p) enabling sensitive BCA detection with AUC being 0.84 and 0.81 in the training and validation phase, respectively. Moreover, DxScore significantly differed in the various histopathological subgroups of BCA and decreased post-operatively. In conclusion, we identified and independently validated cell-free urinary miRNAs as promising biomarkers enabling non-invasive detection of BCA.
ER  -

JURÁČEK, Jaroslav, Barbora PELTANOVÁ, Jan DOLEŽEL, Michal FEDORKO, Dalibor PACÍK, Lenka RADOVÁ, Petra VESELA, Marek SVOBODA, Ondřej SLABÝ a Michal STANÍK. Genome-wide identification of urinary cell-free microRNAs for non-invasive detection of bladder cancer. \textit{Journal of Cellular and Molecular Medicine}. Hoboken, NJ USA: Wiley, 2018, roč.~22, č.~3, s.~2033-2038. ISSN~1582-4934. Dostupné z: https://dx.doi.org/10.1111/jcmm.13487.

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