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@article{1486377,
author = {Šínová, Romana and Kudová, Jana and Nešporová, Kristina and Sergej, Karel and Šuláková, Romana and Velebný, Vladimír and Kubala, Lukáš and Karel, Sergej and Šuláková, Romana},
article_location = {Hoboken},
article_number = {8},
doi = {http://dx.doi.org/10.1002/jcp.27992},
keywords = {dynorphins; enkephalins; heart regeneration; mouse embryonic stem cells; opioid receptors},
language = {eng},
issn = {0021-9541},
journal = {Journal of cellular physiology},
title = {Opioid receptors and opioid peptides in the cardiomyogenesis of mouse embryonic stem cells},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.27992},
volume = {234},
year = {2019}
}
TY - JOUR
ID - 1486377
AU - Šínová, Romana - Kudová, Jana - Nešporová, Kristina - Sergej, Karel - Šuláková, Romana - Velebný, Vladimír - Kubala, Lukáš - Karel, Sergej - Šuláková, Romana
PY - 2019
TI - Opioid receptors and opioid peptides in the cardiomyogenesis of mouse embryonic stem cells
JF - Journal of cellular physiology
VL - 234
IS - 8
SP - 13209-13219
EP - 13209-13219
PB - Wiley
SN - 00219541
KW - dynorphins
KW - enkephalins
KW - heart regeneration
KW - mouse embryonic stem cells
KW - opioid receptors
UR - https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.27992
L2 - https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.27992
N2 - The stimulation of myocardium repair is restricted due to the limited understanding of heart regeneration. Interestingly, endogenous opioid peptides such as dynorphins and enkephalins are suggested to support this process. However, the mechanismwhether through the stimulation of the regenerative capacity of cardiac stem cells or through effects on other cell types in the heartis still not completely understood. Thus, a model of the spontaneous cardiomyogenic differentiation of mouse embryonic stem (mES) cells via the formation of embryoid bodies was used to describe changes in the expression and localization of opioid receptors within cells during the differentiation process and the potential of the selected opioid peptides, dynorphin A and B, and methionin-enkephalins and leucin-enkephalins, to modulate cardiomyogenic differentiation in vitro. The expressions of both - and -opioid receptors significantly increased during mES cell differentiation. Moreover, their primary colocalization with the nucleus was followed by their growing presence on the cytoplasmic membrane with increasing mES cell differentiation status. Interestingly, dynorphin B enhanced the downregulation gene expression of Oct4 characteristic of the pluripotent phenotype. Further, dynorphin B also increased cardiomyocyte-specific Nkx2.5 gene expression. However, neither dynorphin A nor methionin-enkephalins and leucin-enkephalins exhibited any significant effects on the course of mES cell differentiation. In conclusion, despite the increased expression of opioid receptors and some enhancement of mES cell differentiation by dynorphin B, the overall data do not support the notion that opioid peptides have a significant potential to promote the spontaneous cardiomyogenesis of mES cells in vitro.
ER -
ŠÍNOVÁ, Romana, Jana KUDOVÁ, Kristina NEŠPOROVÁ, Karel SERGEJ, Romana ŠULÁKOVÁ, Vladimír VELEBNÝ a Lukáš KUBALA. Opioid receptors and opioid peptides in the cardiomyogenesis of mouse embryonic stem cells. \textit{Journal of cellular physiology}. Hoboken: Wiley, 2019, roč.~234, č.~8, s.~13209-13219. ISSN~0021-9541. Dostupné z: https://dx.doi.org/10.1002/jcp.27992.
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