| FOJTŮ, Michaela, Jan BALVAN, Martina RAUDENSKÁ, Tomáš VIČAR, Daniel BOUSA, Zdeněk SOFER, Michal MASAŘÍK and Martin PUMERA. Black Phosphorus Cytotoxicity Assessments Pitfalls: Advantages and Disadvantages of Metabolic and Morphological Assays. Chemistry - A European Journal. WEINHEIM: Wiley, 2019, vol. 25, No 1, p. 349-360. ISSN 0947-6539. Available from: https://dx.doi.org/10.1002/chem.201804434. |
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@article{1486913,
author = {Fojtů, Michaela and Balvan, Jan and Raudenská, Martina and Vičar, Tomáš and Bousa, Daniel and Sofer, Zdeněk and Masařík, Michal and Pumera, Martin},
article_location = {WEINHEIM},
article_number = {1},
doi = {http://dx.doi.org/10.1002/chem.201804434},
keywords = {2D nanomaterials; black phosphorus; cytotoxicity; holographic microscopy; interference; nanoparticles; viability},
language = {eng},
issn = {0947-6539},
journal = {Chemistry - A European Journal},
title = {Black Phosphorus Cytotoxicity Assessments Pitfalls: Advantages and Disadvantages of Metabolic and Morphological Assays},
url = {http://dx.doi.org/10.1002/chem.201804434},
volume = {25},
year = {2019}
}
TY - JOUR
ID - 1486913
AU - Fojtů, Michaela - Balvan, Jan - Raudenská, Martina - Vičar, Tomáš - Bousa, Daniel - Sofer, Zdeněk - Masařík, Michal - Pumera, Martin
PY - 2019
TI - Black Phosphorus Cytotoxicity Assessments Pitfalls: Advantages and Disadvantages of Metabolic and Morphological Assays
JF - Chemistry - A European Journal
VL - 25
IS - 1
SP - 349-360
EP - 349-360
PB - Wiley
SN - 09476539
KW - 2D nanomaterials
KW - black phosphorus
KW - cytotoxicity
KW - holographic microscopy
KW - interference
KW - nanoparticles
KW - viability
UR - http://dx.doi.org/10.1002/chem.201804434
L2 - http://dx.doi.org/10.1002/chem.201804434
N2 - Black phosphorus (BP) belongs to a group of 2D nanomaterials and nowadays attracts constantly increasing attention. Parallel to the growing utilization of BP nanomaterial increase also the requirements for the thorough comprehension of its potential impact on human and animal health. The aim of this study was to compare and discuss five assays commonly used for the cytotoxicity assessments of nanomaterials with a special focus on BP nanoparticles. A comprehensive survey of factors and pitfalls is provided that should be accounted for when assessing their toxicity and pointed to their inconsistency. BP might introduce various levels of interference during toxicity assessments depending on its concentration applied. More importantly, the BP toxicity evaluation was found to be influenced by the nature of assay chosen. These are based on different principles and do not have to assess all the cellular events equally. A commercial assay based on the measurement of protease activity was identified to be the most suitable for the BP toxicity assessment. Further, the benefit of time-lapse quantitative phase imaging for nanomaterial toxicity evaluation was highlighted. Unlike the conventional assessments it provides real-time analysis of the processes accompanying BP administration and enables to understand them deeper and in the context.
ER -
FOJTŮ, Michaela, Jan BALVAN, Martina RAUDENSKÁ, Tomáš VIČAR, Daniel BOUSA, Zdeněk SOFER, Michal MASAŘÍK and Martin PUMERA. Black Phosphorus Cytotoxicity Assessments Pitfalls: Advantages and Disadvantages of Metabolic and Morphological Assays. \textit{Chemistry - A European Journal}. WEINHEIM: Wiley, 2019, vol.~25, No~1, p.~349-360. ISSN~0947-6539. Available from: https://dx.doi.org/10.1002/chem.201804434.
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