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@article{1489200,
author = {Lo Re, Oriana and Mazza, Tommaso and Vinciguerra, Manlio},
article_location = {LAUSANNE},
article_number = {654},
doi = {http://dx.doi.org/10.3389/fgene.2018.00654},
keywords = {metabolic stress; obesity; MACROD2; irradiation; genotoxic stress response; knock out mouse model},
language = {eng},
issn = {1664-8021},
journal = {FRONTIERS IN GENETICS},
title = {Mono-ADP-Ribosylhydrolase MACROD2 Is Dispensable for Murine Responses to Metabolic and Genotoxic Insults},
url = {http://dx.doi.org/10.3389/fgene.2018.00654},
volume = {9},
year = {2018}
}
TY - JOUR
ID - 1489200
AU - Lo Re, Oriana - Mazza, Tommaso - Vinciguerra, Manlio
PY - 2018
TI - Mono-ADP-Ribosylhydrolase MACROD2 Is Dispensable for Murine Responses to Metabolic and Genotoxic Insults
JF - FRONTIERS IN GENETICS
VL - 9
IS - 654
SP - 1-9
EP - 1-9
PB - FRONTIERS MEDIA SA
SN - 16648021
KW - metabolic stress
KW - obesity
KW - MACROD2
KW - irradiation
KW - genotoxic stress response
KW - knock out mouse model
UR - http://dx.doi.org/10.3389/fgene.2018.00654
N2 - ADP-ribosylation is an important post-translational protein modification that regulates diverse biological processes, controlled by dedicated transferases, and hydrolases. Disruption in the gene encoding for MACROD2, a mono-ADP-ribosylhydrolase, has been associated to the Kabuki syndrome, a pediatric congenital disorder characterized by facial anomalies, and mental retardation. Non-coding and structural mutations/variations in MACROD2 have been associated to psychiatric disorders, to obesity, and to cancer. Mechanistically, it has been recently shown that frequent deletions of the MACROD2 alter DNA repair and sensitivity to DNA damage, resulting in chromosome instability, and colorectal tumorigenesis. Whether MACROD2 deletion sensitizes the organism to metabolic and tumorigenic stressors, in absence of other genetic drivers, is unclear. As MACROD2 is ubiquitously expressed in mice, here we generated constitutively whole-body knock-out mice for MACROD2, starting from mouse embryonic stem (ES) cells deleted for the gene using the VelociGeneo (R) technology, belonging to the Knockout Mouse Project (KOMP) repository, a NIH initiative. MACROD2 knock-out mice were viable and healthy, indistinguishable from wild type littermates. High-fat diet administration induced obesity, and glucose/insulin intolerance in mice independent of MACROD2 gene deletion. Moreover, sub-lethal irradiation did not indicate a survival or lethality bias in MACROD2 knock-out mice compared to wild type littermates. Altogether, our data point against a sufficient role of MACROD2 deletion in aggravating high-fat induced obesity and DNA damage-associated lethality, in absence of other genetic drivers.
ER -
LO RE, Oriana, Tommaso MAZZA a Manlio VINCIGUERRA. Mono-ADP-Ribosylhydrolase MACROD2 Is Dispensable for Murine Responses to Metabolic and Genotoxic Insults. \textit{FRONTIERS IN GENETICS}. LAUSANNE: FRONTIERS MEDIA SA, 2018, roč.~9, č.~654, s.~1-9. ISSN~1664-8021. Dostupné z: https://dx.doi.org/10.3389/fgene.2018.00654.
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