Extensive Genetic Commonality among Wildlife, Wastewater,
Community, and Nosocomial Isolates of Escherichia coli Sequence
Type 131 (H30R1 and H30Rx Subclones) That Carry bla(CTX-M-27)
or bla(CTX-M-15)

J 2018

Extensive Genetic Commonality among Wildlife, Wastewater, Community, and Nosocomial Isolates of Escherichia coli Sequence Type 131 (H30R1 and H30Rx Subclones) That Carry bla(CTX-M-27) or bla(CTX-M-15)

JAMBOROVA, Ivana, Brian D. JOHNSTON, Ivo PAPOUSEK, Katerina KACHLIKOVA, Lenka MICENKOVÁ et. al.

Základní údaje

Originální název

Extensive Genetic Commonality among Wildlife, Wastewater, Community, and Nosocomial Isolates of Escherichia coli Sequence Type 131 (H30R1 and H30Rx Subclones) That Carry bla(CTX-M-27) or bla(CTX-M-15)

Autoři

JAMBOROVA, Ivana (203 Česká republika, garant), Brian D. JOHNSTON (840 Spojené státy), Ivo PAPOUSEK (203 Česká republika), Katerina KACHLIKOVA (203 Česká republika), Lenka MICENKOVÁ (703 Slovensko, domácí), Connie CLABOTS (840 Spojené státy), Anna SKALOVA (203 Česká republika), Katerina CHUDEJOVA (203 Česká republika), Monika DOLEJSKA (203 Česká republika), Ivan LITERAK (203 Česká republika) a James R. JOHNSON (203 Česká republika)

Vydání

Antimicrobial Agents and Chemotherapy, Washington, American Society for Microbiology, 2018, 0066-4804

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.715

Kód RIV

RIV/00216224:14310/18:00105836

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1128/AAC.00519-18

UT WoS

000445405500011

Klíčová slova anglicky

Escherichia coli ST131; ESBL; virulence; nosocomial and community-acquired infections; wildlife; environment

Štítky

14110513, podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 4. 2024 14:07, Mgr. Michal Petr

Anotace

V originále

Escherichia coli sequence type 131 (ST131) is currently one of the leading causes of multidrug-resistant extraintestinal infections globally. Here, we analyzed the phenotypic and genotypic characteristics of 169 ST131 isolates from various sources (wildlife, wastewater, companion animals, community, and hospitals) to determine whether wildlife and the environment share similar strains with humans, supporting transmission of ST131 between different ecological niches. Susceptibility to 32 antimicro-bials was tested by disc diffusion and broth microdilution. Antibiotic resistance genes, integrons, plasmid replicons, 52 virulence genes, and fimH-based subtypes were detected by PCR and DNA sequencing. Genomic relatedness was determined by pulsed-field gel electrophoresis (PFGE). The genetic context and plasmid versus chromosomal location of extended-spectrum beta-lactamase and AmpC beta-lactamase genes was determined by PCR and probe hybridization, respectively. The 169 ST131 study isolates segregated predominantly into bla(CTX-M-15) H30Rx (60%) and bla(CTX-M-27) H30R1 (25%) subclones. Within each subclone, isolates from different source groups were categorized into distinct PFGE clusters; genotypic characteristics were fairly well conserved within each major PFGE cluster. Irrespective of source, the bla(CTX-M-15) H30Rx isolates typically exhibited virotype A (89%), an F2:A1:B- replicon (84%), and a 1.7-kb class 1 integron (92%) and had diverse structures upstream of the bla(CTX-M) region. In contrast, the bla(CTX-M-27) H30R1 isolates typically exhibited virotype C (86%), an F1:A2:B20 replicon (76%), and a conserved IS26-Delta ISEcp1-bla(CTX-M)-like structure. Despite considerable overall genetic diversity, our data demonstrate significant commonality between E. coli ST131 isolates from diverse environments, supporting transmission between different sources, including humans, environment, and wildlife.

Návaznosti

EF15_003/0000469, projekt VaV

Název: Cetocoen Plus

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

JAMBOROVA, Ivana, Brian D. JOHNSTON, Ivo PAPOUSEK, Katerina KACHLIKOVA, Lenka MICENKOVÁ, Connie CLABOTS, Anna SKALOVA, Katerina CHUDEJOVA, Monika DOLEJSKA, Ivan LITERAK a James R. JOHNSON. Extensive Genetic Commonality among Wildlife, Wastewater, Community, and Nosocomial Isolates of Escherichia coli Sequence Type 131 (H30R1 and H30Rx Subclones) That Carry bla(CTX-M-27) or bla(CTX-M-15). Antimicrobial Agents and Chemotherapy. Washington: American Society for Microbiology, 2018, roč. 62, č. 10, s. 1-15. ISSN 0066-4804. Dostupné z: https://dx.doi.org/10.1128/AAC.00519-18.

@article{1489820,
   author = {Jamborova, Ivana and Johnston, Brian D. and Papousek, Ivo and Kachlikova, Katerina and Micenková, Lenka and Clabots, Connie and Skalova, Anna and Chudejova, Katerina and Dolejska, Monika and Literak, Ivan and Johnson, James R.},
   article_location = {Washington},
   article_number = {10},
   doi = {http://dx.doi.org/10.1128/AAC.00519-18},
   keywords = {Escherichia coli ST131; ESBL; virulence; nosocomial and community-acquired infections; wildlife; environment},
   language = {eng},
   issn = {0066-4804},
   journal = {Antimicrobial Agents and Chemotherapy},
   title = {Extensive Genetic Commonality among Wildlife, Wastewater, Community, and Nosocomial Isolates of Escherichia coli Sequence Type 131 (H30R1 and H30Rx Subclones) That Carry bla(CTX-M-27) or bla(CTX-M-15)},
   url = {http://dx.doi.org/10.1128/AAC.00519-18},
   volume = {62},
   year = {2018}
}

TY  - JOUR
ID  - 1489820
AU  - Jamborova, Ivana - Johnston, Brian D. - Papousek, Ivo - Kachlikova, Katerina - Micenková, Lenka - Clabots, Connie - Skalova, Anna - Chudejova, Katerina - Dolejska, Monika - Literak, Ivan - Johnson, James R.
PY  - 2018
TI  - Extensive Genetic Commonality among Wildlife, Wastewater, Community, and Nosocomial Isolates of Escherichia coli Sequence Type 131 (H30R1 and H30Rx Subclones) That Carry bla(CTX-M-27) or bla(CTX-M-15)
JF  - Antimicrobial Agents and Chemotherapy
VL  - 62
IS  - 10
SP  - 1-15
EP  - 1-15
PB  - American Society for Microbiology
SN  - 00664804
KW  - Escherichia coli ST131
KW  - ESBL
KW  - virulence
KW  - nosocomial and community-acquired infections
KW  - wildlife
KW  - environment
UR  - http://dx.doi.org/10.1128/AAC.00519-18
N2  - Escherichia coli sequence type 131 (ST131) is currently one of the leading causes of multidrug-resistant extraintestinal infections globally. Here, we analyzed the phenotypic and genotypic characteristics of 169 ST131 isolates from various sources (wildlife, wastewater, companion animals, community, and hospitals) to determine whether wildlife and the environment share similar strains with humans, supporting transmission of ST131 between different ecological niches. Susceptibility to 32 antimicro-bials was tested by disc diffusion and broth microdilution. Antibiotic resistance genes, integrons, plasmid replicons, 52 virulence genes, and fimH-based subtypes were detected by PCR and DNA sequencing. Genomic relatedness was determined by pulsed-field gel electrophoresis (PFGE). The genetic context and plasmid versus chromosomal location of extended-spectrum beta-lactamase and AmpC beta-lactamase genes was determined by PCR and probe hybridization, respectively. The 169 ST131 study isolates segregated predominantly into bla(CTX-M-15) H30Rx (60%) and bla(CTX-M-27) H30R1 (25%) subclones. Within each subclone, isolates from different source groups were categorized into distinct PFGE clusters; genotypic characteristics were fairly well conserved within each major PFGE cluster. Irrespective of source, the bla(CTX-M-15) H30Rx isolates typically exhibited virotype A (89%), an F2:A1:B- replicon (84%), and a 1.7-kb class 1 integron (92%) and had diverse structures upstream of the bla(CTX-M) region. In contrast, the bla(CTX-M-27) H30R1 isolates typically exhibited virotype C (86%), an F1:A2:B20 replicon (76%), and a conserved IS26-Delta ISEcp1-bla(CTX-M)-like structure. Despite considerable overall genetic diversity, our data demonstrate significant commonality between E. coli ST131 isolates from diverse environments, supporting transmission between different sources, including humans, environment, and wildlife.
ER  -

JAMBOROVA, Ivana, Brian D. JOHNSTON, Ivo PAPOUSEK, Katerina KACHLIKOVA, Lenka MICENKOVÁ, Connie CLABOTS, Anna SKALOVA, Katerina CHUDEJOVA, Monika DOLEJSKA, Ivan LITERAK a James R. JOHNSON. Extensive Genetic Commonality among Wildlife, Wastewater, Community, and Nosocomial Isolates of Escherichia coli Sequence Type 131 (H30R1 and H30Rx Subclones) That Carry bla(CTX-M-27) or bla(CTX-M-15). \textit{Antimicrobial Agents and Chemotherapy}. Washington: American Society for Microbiology, 2018, roč.~62, č.~10, s.~1-15. ISSN~0066-4804. Dostupné z: https://dx.doi.org/10.1128/AAC.00519-18.

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