Identification of microRNAs differentially expressed in
glioblastoma stem-like cells and their association with patient
survival

J 2018

Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival

ŠÁNA, Jiří, Petr BUŠEK, Pavel FADRUS, Andrej BEŠŠE, Lenka RADOVÁ et. al.

Základní údaje

Originální název

Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival

Autoři

ŠÁNA, Jiří (203 Česká republika, domácí), Petr BUŠEK (203 Česká republika), Pavel FADRUS (203 Česká republika, domácí), Andrej BEŠŠE (703 Slovensko, domácí), Lenka RADOVÁ (203 Česká republika, domácí), Marek VEČEŘA (703 Slovensko, domácí), Stefan REGULI (203 Česká republika), Lucie SROMOVA STOLLINOVA (203 Česká republika), Marek HILSER (203 Česká republika), Radim LIPINA (203 Česká republika, domácí), Radek LAKOMÝ (203 Česká republika, domácí), Leoš KŘEN (203 Česká republika, domácí), Martin SMRČKA (203 Česká republika, domácí), Aleksi ŠEDO (203 Česká republika) a Ondřej SLABÝ (203 Česká republika, garant, domácí)

Vydání

Scientific reports, LONDON, NATURE PUBLISHING GROUP, 2018, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30204 Oncology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.011

Kód RIV

RIV/00216224:14740/18:00105841

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1038/s41598-018-20929-6

UT WoS

000424743500052

Klíčová slova anglicky

Glioblastoma stem-like cells

Štítky

14110224, 14110230, 14110811, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 3. 2019 13:29, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Glioblastoma stem-like cells (GSCs) are critical for the aggressiveness and progression of glioblastoma (GBM) and contribute to its resistance to adjuvant treatment. MicroRNAs (miRNAs) are small, non-coding RNAs controlling gene expression at the post-transcriptional level, which are known to be important regulators of the stem-like features. Moreover, miRNAs have been previously proved to be promising diagnostic biomarkers in several cancers including GBM. Using global expression analysis of miRNAs in 10 paired in-vitro as well as in-vivo characterized primary GSC and non-stem glioblastoma cultures, we identified a miRNA signature associated with the stem-like phenotype in GBM. 51 most deregulated miRNAs classified the cell cultures into GSC and non-stem cell clusters and identified a subgroup of GSC cultures with more pronounced stem-cell characteristics. The importance of the identified miRNA signature was further supported by demonstrating that a Risk Score based on the expression of seven miRNAs overexpressed in GSC predicted overall survival in GBM patients in the TCGA dataset independently of the IDH1 status. In summary, we identified miRNAs differentially expressed in GSCs and described their association with GBM patient survival. We propose that these miRNAs participate on GSC features and could represent helpful prognostic markers and potential therapeutic targets in GBM.

Návaznosti

LM2015064, projekt VaV

Název: Český národní uzel Evropské infrastruktury pro translační medicínu (Akronym: EATRIS-ERIC-CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Involvement of Czech Translational Medicine Infrastructure to the European Advanced Translational Research Infrastructure in Medicine

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

NV15-33158A, projekt VaV

Název: Nová úroveň molekulární taxonomie glioblastomu založená na expresních profilech dlouhých nekódujících RNA: implikace pro diagnostiku a terapii

NV15-34553A, projekt VaV

Název: Využití mikroRNA v mozkomíšním moku pro diagnostiku mozkových nádorů

Citovat

ŠÁNA, Jiří, Petr BUŠEK, Pavel FADRUS, Andrej BEŠŠE, Lenka RADOVÁ, Marek VEČEŘA, Stefan REGULI, Lucie SROMOVA STOLLINOVA, Marek HILSER, Radim LIPINA, Radek LAKOMÝ, Leoš KŘEN, Martin SMRČKA, Aleksi ŠEDO a Ondřej SLABÝ. Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival. Scientific reports. LONDON: NATURE PUBLISHING GROUP, 2018, roč. 8, FEB, s. 2836-2846. ISSN 2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-018-20929-6.

@article{1489878,
   author = {Šána, Jiří and Bušek, Petr and Fadrus, Pavel and Bešše, Andrej and Radová, Lenka and Večeřa, Marek and Reguli, Stefan and Sromova Stollinova, Lucie and Hilser, Marek and Lipina, Radim and Lakomý, Radek and Křen, Leoš and Smrčka, Martin and Šedo, Aleksi and Slabý, Ondřej},
   article_location = {LONDON},
   article_number = {FEB},
   doi = {http://dx.doi.org/10.1038/s41598-018-20929-6},
   keywords = {Glioblastoma stem-like cells},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific reports},
   title = {Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival},
   volume = {8},
   year = {2018}
}

TY  - JOUR
ID  - 1489878
AU  - Šána, Jiří - Bušek, Petr - Fadrus, Pavel - Bešše, Andrej - Radová, Lenka - Večeřa, Marek - Reguli, Stefan - Sromova Stollinova, Lucie - Hilser, Marek - Lipina, Radim - Lakomý, Radek - Křen, Leoš - Smrčka, Martin - Šedo, Aleksi - Slabý, Ondřej
PY  - 2018
TI  - Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival
JF  - Scientific reports
VL  - 8
IS  - FEB
SP  - 2836
EP  - 2836
PB  - NATURE PUBLISHING GROUP
SN  - 20452322
KW  - Glioblastoma stem-like cells
N2  - Glioblastoma stem-like cells (GSCs) are critical for the aggressiveness and progression of glioblastoma (GBM) and contribute to its resistance to adjuvant treatment. MicroRNAs (miRNAs) are small, non-coding RNAs controlling gene expression at the post-transcriptional level, which are known to be important regulators of the stem-like features. Moreover, miRNAs have been previously proved to be promising diagnostic biomarkers in several cancers including GBM. Using global expression analysis of miRNAs in 10 paired in-vitro as well as in-vivo characterized primary GSC and non-stem glioblastoma cultures, we identified a miRNA signature associated with the stem-like phenotype in GBM. 51 most deregulated miRNAs classified the cell cultures into GSC and non-stem cell clusters and identified a subgroup of GSC cultures with more pronounced stem-cell characteristics. The importance of the identified miRNA signature was further supported by demonstrating that a Risk Score based on the expression of seven miRNAs overexpressed in GSC predicted overall survival in GBM patients in the TCGA dataset independently of the IDH1 status. In summary, we identified miRNAs differentially expressed in GSCs and described their association with GBM patient survival. We propose that these miRNAs participate on GSC features and could represent helpful prognostic markers and potential therapeutic targets in GBM.
ER  -

ŠÁNA, Jiří, Petr BUŠEK, Pavel FADRUS, Andrej BEŠŠE, Lenka RADOVÁ, Marek VEČEŘA, Stefan REGULI, Lucie SROMOVA STOLLINOVA, Marek HILSER, Radim LIPINA, Radek LAKOMÝ, Leoš KŘEN, Martin SMRČKA, Aleksi ŠEDO a Ondřej SLABÝ. Identification of microRNAs differentially expressed in glioblastoma stem-like cells and their association with patient survival. \textit{Scientific reports}. LONDON: NATURE PUBLISHING GROUP, 2018, roč.~8, FEB, s.~2836-2846. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-018-20929-6.

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