MicroRNA isolation and quantification in cerebrospinal fluid: A
comparative methodical study

J 2018

MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study

KOPKOVÁ, Alena, Jiří ŠÁNA, Pavel FADRUS, Táňa MACHÁČKOVÁ, Marek VEČEŘA et. al.

Základní údaje

Originální název

MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study

Autoři

KOPKOVÁ, Alena (203 Česká republika, domácí), Jiří ŠÁNA (203 Česká republika, domácí), Pavel FADRUS (203 Česká republika, domácí), Táňa MACHÁČKOVÁ (203 Česká republika, domácí), Marek VEČEŘA (703 Slovensko, domácí), Václav VYBÍHAL (203 Česká republika, domácí), Jaroslav JURÁČEK (203 Česká republika, domácí), Petra VYCHYTILOVÁ (203 Česká republika, domácí), Martin SMRČKA (203 Česká republika, domácí) a Ondřej SLABÝ (203 Česká republika, garant, domácí)

Vydání

Plos one, San Francisco, Public Library of Science, 2018, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30204 Oncology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.776

Kód RIV

RIV/00216224:14740/18:00106965

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1371/journal.pone.0208580

UT WoS

000452640900023

Klíčová slova anglicky

MicroRNA; cerebrospinal fluid

Štítky

14110224, 14110811, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 5. 2019 15:00, Soňa Böhmová

Anotace

V originále

Associated with the pathogenesis of many cancers, including brain tumors, microRNAs (miRNAs) present promising diagnostic biomarkers. These molecules have been also studied in cerebrospinal fluid (CSF), showing great potential as a diagnostic tool in patients with brain tumors. Even though there are some biological and technological factors that could affect the results and their biological and clinical interpretation, miRNA analysis in CSF is not fully standardized. This study aims to compare several RNA extraction and miRNA quantification approaches, including high-throughput technologies and individual miRNA detection methods, thereby contributing to the optimization and standardization of quantification of extracellular miRNAs in CSF. Such knowledge is essential for the potential use of miRNAs as diagnostic biomarkers in brain tumors.

Návaznosti

NV15-34553A, projekt VaV

Název: Využití mikroRNA v mozkomíšním moku pro diagnostiku mozkových nádorů

Citovat

KOPKOVÁ, Alena, Jiří ŠÁNA, Pavel FADRUS, Táňa MACHÁČKOVÁ, Marek VEČEŘA, Václav VYBÍHAL, Jaroslav JURÁČEK, Petra VYCHYTILOVÁ, Martin SMRČKA a Ondřej SLABÝ. MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study. Plos one. San Francisco: Public Library of Science, 2018, roč. 13, č. 12, s. 1-12. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0208580.

@article{1490159,
   author = {Kopková, Alena and Šána, Jiří and Fadrus, Pavel and Macháčková, Táňa and Večeřa, Marek and Vybíhal, Václav and Juráček, Jaroslav and Vychytilová, Petra and Smrčka, Martin and Slabý, Ondřej},
   article_location = {San Francisco},
   article_number = {12},
   doi = {http://dx.doi.org/10.1371/journal.pone.0208580},
   keywords = {MicroRNA; cerebrospinal fluid},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study},
   volume = {13},
   year = {2018}
}

TY  - JOUR
ID  - 1490159
AU  - Kopková, Alena - Šána, Jiří - Fadrus, Pavel - Macháčková, Táňa - Večeřa, Marek - Vybíhal, Václav - Juráček, Jaroslav - Vychytilová, Petra - Smrčka, Martin - Slabý, Ondřej
PY  - 2018
TI  - MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study
JF  - Plos one
VL  - 13
IS  - 12
SP  - 1-12
EP  - 1-12
PB  - Public Library of Science
SN  - 19326203
KW  - MicroRNA
KW  - cerebrospinal fluid
N2  - Associated with the pathogenesis of many cancers, including brain tumors, microRNAs (miRNAs) present promising diagnostic biomarkers. These molecules have been also studied in cerebrospinal fluid (CSF), showing great potential as a diagnostic tool in patients with brain tumors. Even though there are some biological and technological factors that could affect the results and their biological and clinical interpretation, miRNA analysis in CSF is not fully standardized. This study aims to compare several RNA extraction and miRNA quantification approaches, including high-throughput technologies and individual miRNA detection methods, thereby contributing to the optimization and standardization of quantification of extracellular miRNAs in CSF. Such knowledge is essential for the potential use of miRNAs as diagnostic biomarkers in brain tumors.
ER  -

KOPKOVÁ, Alena, Jiří ŠÁNA, Pavel FADRUS, Táňa MACHÁČKOVÁ, Marek VEČEŘA, Václav VYBÍHAL, Jaroslav JURÁČEK, Petra VYCHYTILOVÁ, Martin SMRČKA a Ondřej SLABÝ. MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study. \textit{Plos one}. San Francisco: Public Library of Science, 2018, roč.~13, č.~12, s.~1-12. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0208580.

Podrobný výpis o publikaci