Genomagnetic LAMP-based electrochemical test for determination
of high-risk HPV16 and HPV18 in clinical samples

J 2018

Genomagnetic LAMP-based electrochemical test for determination of high-risk HPV16 and HPV18 in clinical samples

BARTOŠÍK, Martin, Ludmila JIRÁKOVÁ, Milan ANTON, Bořivoj VOJTĚŠEK, Roman HRSTKA et. al.

Základní údaje

Originální název

Genomagnetic LAMP-based electrochemical test for determination of high-risk HPV16 and HPV18 in clinical samples

Autoři

BARTOŠÍK, Martin (703 Slovensko, garant), Ludmila JIRÁKOVÁ (203 Česká republika), Milan ANTON (203 Česká republika, domácí), Bořivoj VOJTĚŠEK (203 Česká republika) a Roman HRSTKA (203 Česká republika)

Vydání

Analytica Chimica Acta, Amsterdam, Elsevier Science publishers, 2018, 0003-2670

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30214 Obstetrics and gynaecology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.256

Kód RIV

RIV/00216224:14110/18:00105867

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.aca.2018.08.020

UT WoS

000448290800005

Klíčová slova anglicky

HPV; Cervical cancer; Genomagnetic assay; Electrode chip; LAMP amplification

Štítky

14110411, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 4. 2024 10:09, Mgr. Michal Petr

Anotace

V originále

Major cause of cervical cancer is a persistent infection with high-risk types of human papillomaviruses (HPV). For that reason, HPV testing is now becoming an important addition to standard cytological screening of cervical malignancies in women (known as Pap test). New methods are sought which could offer rapid and inexpensive detection schemes, such as those based on electrochemical (EC) readout. Here, we developed an assay for parallel detection of two most oncogenic high-risk HPV types, HPV16 and HPV18, by combining loop-mediated amplification (LAMP) of template DNA, its separation using magnetic beads and detection with amperometry at carbon-based electrode chips. Our EC-LAMP test enabled us to successfully discriminate both HPV types not only in cancer cell lines, but also using clinical material obtained from HPV-positive patient samples. (C 2018 Elsevier B.V. All rights reserved.

Citovat

BARTOŠÍK, Martin, Ludmila JIRÁKOVÁ, Milan ANTON, Bořivoj VOJTĚŠEK a Roman HRSTKA. Genomagnetic LAMP-based electrochemical test for determination of high-risk HPV16 and HPV18 in clinical samples. Analytica Chimica Acta. Amsterdam: Elsevier Science publishers, 2018, roč. 1042, DEC 26 2018, s. 37-43. ISSN 0003-2670. Dostupné z: https://dx.doi.org/10.1016/j.aca.2018.08.020.

@article{1490403,
   author = {Bartošík, Martin and Jiráková, Ludmila and Anton, Milan and Vojtěšek, Bořivoj and Hrstka, Roman},
   article_location = {Amsterdam},
   article_number = {DEC 26 2018},
   doi = {http://dx.doi.org/10.1016/j.aca.2018.08.020},
   keywords = {HPV; Cervical cancer; Genomagnetic assay; Electrode chip; LAMP amplification},
   language = {eng},
   issn = {0003-2670},
   journal = {Analytica Chimica Acta},
   title = {Genomagnetic LAMP-based electrochemical test for determination of high-risk HPV16 and HPV18 in clinical samples},
   url = {https://www.sciencedirect.com/science/article/pii/S0003267018309723},
   volume = {1042},
   year = {2018}
}

TY  - JOUR
ID  - 1490403
AU  - Bartošík, Martin - Jiráková, Ludmila - Anton, Milan - Vojtěšek, Bořivoj - Hrstka, Roman
PY  - 2018
TI  - Genomagnetic LAMP-based electrochemical test for determination of high-risk HPV16 and HPV18 in clinical samples
JF  - Analytica Chimica Acta
VL  - 1042
IS  - DEC 26 2018
SP  - 37-43
EP  - 37-43
PB  - Elsevier Science publishers
SN  - 00032670
KW  - HPV
KW  - Cervical cancer
KW  - Genomagnetic assay
KW  - Electrode chip
KW  - LAMP amplification
UR  - https://www.sciencedirect.com/science/article/pii/S0003267018309723
N2  - Major cause of cervical cancer is a persistent infection with high-risk types of human papillomaviruses (HPV). For that reason, HPV testing is now becoming an important addition to standard cytological screening of cervical malignancies in women (known as Pap test). New methods are sought which could offer rapid and inexpensive detection schemes, such as those based on electrochemical (EC) readout. Here, we developed an assay for parallel detection of two most oncogenic high-risk HPV types, HPV16 and HPV18, by combining loop-mediated amplification (LAMP) of template DNA, its separation using magnetic beads and detection with amperometry at carbon-based electrode chips. Our EC-LAMP test enabled us to successfully discriminate both HPV types not only in cancer cell lines, but also using clinical material obtained from HPV-positive patient samples. (C 2018 Elsevier B.V. All rights reserved.
ER  -

BARTOŠÍK, Martin, Ludmila JIRÁKOVÁ, Milan ANTON, Bořivoj VOJTĚŠEK a Roman HRSTKA. Genomagnetic LAMP-based electrochemical test for determination of high-risk HPV16 and HPV18 in clinical samples. \textit{Analytica Chimica Acta}. Amsterdam: Elsevier Science publishers, 2018, roč.~1042, DEC 26 2018, s.~37-43. ISSN~0003-2670. Dostupné z: https://dx.doi.org/10.1016/j.aca.2018.08.020.

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