SINHA, Dhiraj, Vitali BIALEVICH, Katsiaryna SHAMAYEVA, Alena GUZANOVA, Alexandra SISÁKOVÁ, Eva CSEFALVAY, David REHA, Lumír KREJČÍ, Jannette CAREY, Marie WEISEROVA a Rüdiger ETTRICH. A residue of motif III positions the helicase domains of motor subunit HsdR in restriction-modification enzyme EcoR124I. JOURNAL OF MOLECULAR MODELING. New York: Springer, 2018, roč. 24, č. 7, s. 1-11. ISSN 1610-2940. Dostupné z: https://dx.doi.org/10.1007/s00894-018-3722-8. |
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@article{1490539, author = {Sinha, Dhiraj and Bialevich, Vitali and Shamayeva, Katsiaryna and Guzanova, Alena and Sisáková, Alexandra and Csefalvay, Eva and Reha, David and Krejčí, Lumír and Carey, Jannette and Weiserova, Marie and Ettrich, Rüdiger}, article_location = {New York}, article_number = {7}, doi = {http://dx.doi.org/10.1007/s00894-018-3722-8}, keywords = {DNA restriction enzymes; Molecular mechanics; Molecular modeling; Principal components analysis; Multisubunit enzyme complex; Domain interactions}, language = {eng}, issn = {1610-2940}, journal = {JOURNAL OF MOLECULAR MODELING}, title = {A residue of motif III positions the helicase domains of motor subunit HsdR in restriction-modification enzyme EcoR124I}, volume = {24}, year = {2018} }
TY - JOUR ID - 1490539 AU - Sinha, Dhiraj - Bialevich, Vitali - Shamayeva, Katsiaryna - Guzanova, Alena - Sisáková, Alexandra - Csefalvay, Eva - Reha, David - Krejčí, Lumír - Carey, Jannette - Weiserova, Marie - Ettrich, Rüdiger PY - 2018 TI - A residue of motif III positions the helicase domains of motor subunit HsdR in restriction-modification enzyme EcoR124I JF - JOURNAL OF MOLECULAR MODELING VL - 24 IS - 7 SP - 1-11 EP - 1-11 PB - Springer SN - 16102940 KW - DNA restriction enzymes KW - Molecular mechanics KW - Molecular modeling KW - Principal components analysis KW - Multisubunit enzyme complex KW - Domain interactions N2 - Type I restriction-modification enzymes differ significantly from the type II enzymes commonly used as molecular biology reagents. On hemi-methylated DNAs type I enzymes like the EcoR124I restriction-modification complex act as conventional adenine methylases at their specific target sequences, but unmethylated targets induce them to translocate thousands of base pairs through the stationary enzyme before cleaving distant sites nonspecifically. EcoR124I is a superfamily 2 DEAD-box helicase like eukaryotic double-strand DNA translocase Rad54, with two RecA-like helicase domains and seven characteristic sequence motifs that are implicated in translocation. In Rad54 a so-called extended region adjacent to motif III is involved in ATPase activity. Although the EcoR124I extended region bears sequence and structural similarities with Rad54, it does not influence ATPase or restriction activity as shown in this work, but mutagenesis of the conserved glycine residue of its motif III does alter ATPase and DNA cleavage activity. Through the lens of molecular dynamics, a full model of HsdR of EcoR124I based on available crystal structures allowed interpretation of functional effects of mutants in motif III and its extended region. The results indicate that the conserved glycine residue of motif III has a role in positioning the two helicase domains. ER -
SINHA, Dhiraj, Vitali BIALEVICH, Katsiaryna SHAMAYEVA, Alena GUZANOVA, Alexandra SISÁKOVÁ, Eva CSEFALVAY, David REHA, Lumír KREJČÍ, Jannette CAREY, Marie WEISEROVA a Rüdiger ETTRICH. A residue of motif III positions the helicase domains of motor subunit HsdR in restriction-modification enzyme EcoR124I. \textit{JOURNAL OF MOLECULAR MODELING}. New York: Springer, 2018, roč.~24, č.~7, s.~1-11. ISSN~1610-2940. Dostupné z: https://dx.doi.org/10.1007/s00894-018-3722-8.
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