Antimicrobial effect of novel hydrogel matrix based on natural
polysaccharide Sterculia urens

J 2018

Antimicrobial effect of novel hydrogel matrix based on natural polysaccharide Sterculia urens

LIPOVÝ, Břetislav, Jakub HOLOUBEK, Lukáš VACEK, Filip RŮŽIČKA, E. NEDOMOVA et. al.

Základní údaje

Originální název

Antimicrobial effect of novel hydrogel matrix based on natural polysaccharide Sterculia urens

Autoři

LIPOVÝ, Břetislav (203 Česká republika, garant, domácí), Jakub HOLOUBEK (203 Česká republika, domácí), Lukáš VACEK (203 Česká republika, domácí), Filip RŮŽIČKA (203 Česká republika, domácí), E. NEDOMOVA (203 Česká republika), H. POŠTULKOVÁ (203 Česká republika) a L. VOJTOVÁ (203 Česká republika)

Vydání

Čs. epidemiologie, mikrobiologie, imunologie, Praha, ČSL JEP, 2018, 1210-7913

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.397

Kód RIV

RIV/00216224:14110/18:00105964

Organizační jednotka

Lékařská fakulta

UT WoS

000455791100003

Klíčová slova anglicky

Gum Karaya; hydrogel; antimicrobial activity; wound healing; burn wounds

Štítky

14110113, 14110229, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 2. 2019 20:35, Soňa Böhmová

Anotace

V originále

Introduction: Materials for modern wound-management are a very broad and heterogeneous group. One of the most important representatives is natural materials, or more precisely polysaccharides isolated from various plants and animals. With the increasing resistance of pathogens to established antimicrobial agents, there is also an attempt to discover new mechanisms of the effects of these materials. Gum karaya (GK) is a very promising representative of the natural polysaccharides group and, since it is obtained from Sterculla urens as resin, it is also possible to assume its certain antimicrobial activity. Material and methodology: The antimicrobial potential of GK and chitosan (Ch) has been tested on several preselected strains to match the real epidemiological situation of the agents of infectious complications in the field of burned wounds. Tested strains included representatives of gram-positive and gram-negative bacteria as well as selected yeasts. Methicillin susceptible Staphylococcus aureus CCM 4223 (ATCC 29213), methicillin resistant Staphylococcus aureus CCM 4750 (ATCC 43300), Klebsiella pneumoniae CCM 4985 (ATCC 700603). Candida albicans CCM 8261 (ATCC 90028), Pseudomonas aeruginosa CCM 3955 (ATCC 27853) were obtained from the Czech Collection of Microorganisms. Pseudomonas aeruginosa FF 1, Pseudomonas aeruginosa FF 2 and Pseudomonas aeruginosa FF 3 (all multi-resistant clinical strains), Staphylococcus epidermidis A 013, Staphylococcus epidermidis A 117, and Candida parapsilosis BC 11 were obtained from the Collection of Microorganisms at the St. Anne's University Hospital, Brno. Antimicrobial tests were performed using the disk diffusion test methodology. Another set of antimicrobial tests was obtained by measuring the growth curves. Results: Bacteriostatic activity testing showed 1% GK concentration and both 1% and 0.5% chitosan concentration effective against all pathogens tested. The combination of GK s iCh, in concentrations of 1% and 0.5% had similar or better effect. Lower concentrations of the combined material are poorly effective against tested strains. Bactericidal activity testing has not produced positive results, except for Candida spp., where only a partial effect of GK(50)/Ch(50) was observed at 1% concentration. In the growth curve test, the efficiency of both GK alone and chitosan was found to be significantly higher in gram-positive bacteria compared to gram-negative ones. In the case of this experiment, only a one-tenth concentration was used compared to the disk diffusion test concentration. This results correspond with the data from the bacteriostatic activity testing. Conclusion: This is the first publication that attempts to comprehensively define the potential for GK antimicrobial activity and also the possible potentiation of this activity with the use of chitosan. Further experiments are needed to extend the antimicrobial efficiency to gram-negative bacteria.

Návaznosti

NV16-29916A, projekt VaV

Název: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu

ROZV/25/LF/2017, interní kód MU

Název: LF - Příspěvek na IP 2017

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, LF - Příspěvek na IP 2017, Interní rozvojové projekty

Citovat

LIPOVÝ, Břetislav, Jakub HOLOUBEK, Lukáš VACEK, Filip RŮŽIČKA, E. NEDOMOVA, H. POŠTULKOVÁ a L. VOJTOVÁ. Antimicrobial effect of novel hydrogel matrix based on natural polysaccharide Sterculia urens. Čs. epidemiologie, mikrobiologie, imunologie. Praha: ČSL JEP, 2018, roč. 67, č. 4, s. 166-174. ISSN 1210-7913.

@article{1491940,
   author = {Lipový, Břetislav and Holoubek, Jakub and Vacek, Lukáš and Růžička, Filip and Nedomova, E. and Poštulková, H. and Vojtová, L.},
   article_location = {Praha},
   article_number = {4},
   keywords = {Gum Karaya; hydrogel; antimicrobial activity; wound healing; burn wounds},
   language = {eng},
   issn = {1210-7913},
   journal = {Čs. epidemiologie, mikrobiologie, imunologie},
   title = {Antimicrobial effect of novel hydrogel matrix based on natural polysaccharide Sterculia urens},
   volume = {67},
   year = {2018}
}

TY  - JOUR
ID  - 1491940
AU  - Lipový, Břetislav - Holoubek, Jakub - Vacek, Lukáš - Růžička, Filip - Nedomova, E. - Poštulková, H. - Vojtová, L.
PY  - 2018
TI  - Antimicrobial effect of novel hydrogel matrix based on natural polysaccharide Sterculia urens
JF  - Čs. epidemiologie, mikrobiologie, imunologie
VL  - 67
IS  - 4
SP  - 166-174
EP  - 166-174
PB  - ČSL JEP
SN  - 12107913
KW  - Gum Karaya
KW  - hydrogel
KW  - antimicrobial activity
KW  - wound healing
KW  - burn wounds
N2  - Introduction: Materials for modern wound-management are a very broad and heterogeneous group. One of the most important representatives is natural materials, or more precisely polysaccharides isolated from various plants and animals. With the increasing resistance of pathogens to established antimicrobial agents, there is also an attempt to discover new mechanisms of the effects of these materials. Gum karaya (GK) is a very promising representative of the natural polysaccharides group and, since it is obtained from Sterculla urens as resin, it is also possible to assume its certain antimicrobial activity. Material and methodology: The antimicrobial potential of GK and chitosan (Ch) has been tested on several preselected strains to match the real epidemiological situation of the agents of infectious complications in the field of burned wounds. Tested strains included representatives of gram-positive and gram-negative bacteria as well as selected yeasts. Methicillin susceptible Staphylococcus aureus CCM 4223 (ATCC 29213), methicillin resistant Staphylococcus aureus CCM 4750 (ATCC 43300), Klebsiella pneumoniae CCM 4985 (ATCC 700603). Candida albicans CCM 8261 (ATCC 90028), Pseudomonas aeruginosa CCM 3955 (ATCC 27853) were obtained from the Czech Collection of Microorganisms. Pseudomonas aeruginosa FF 1, Pseudomonas aeruginosa FF 2 and Pseudomonas aeruginosa FF 3 (all multi-resistant clinical strains), Staphylococcus epidermidis A 013, Staphylococcus epidermidis A 117, and Candida parapsilosis BC 11 were obtained from the Collection of Microorganisms at the St. Anne's University Hospital, Brno. Antimicrobial tests were performed using the disk diffusion test methodology. Another set of antimicrobial tests was obtained by measuring the growth curves. Results: Bacteriostatic activity testing showed 1% GK concentration and both 1% and 0.5% chitosan concentration effective against all pathogens tested. The combination of GK s iCh, in concentrations of 1% and 0.5% had similar or better effect. Lower concentrations of the combined material are poorly effective against tested strains. Bactericidal activity testing has not produced positive results, except for Candida spp., where only a partial effect of GK(50)/Ch(50) was observed at 1% concentration. In the growth curve test, the efficiency of both GK alone and chitosan was found to be significantly higher in gram-positive bacteria compared to gram-negative ones. In the case of this experiment, only a one-tenth concentration was used compared to the disk diffusion test concentration. This results correspond with the data from the bacteriostatic activity testing. Conclusion: This is the first publication that attempts to comprehensively define the potential for GK antimicrobial activity and also the possible potentiation of this activity with the use of chitosan. Further experiments are needed to extend the antimicrobial efficiency to gram-negative bacteria.
ER  -

LIPOVÝ, Břetislav, Jakub HOLOUBEK, Lukáš VACEK, Filip RŮŽIČKA, E. NEDOMOVA, H. POŠTULKOVÁ a L. VOJTOVÁ. Antimicrobial effect of novel hydrogel matrix based on natural polysaccharide Sterculia urens. \textit{Čs. epidemiologie, mikrobiologie, imunologie}. Praha: ČSL JEP, 2018, roč.~67, č.~4, s.~166-174. ISSN~1210-7913.

Podrobný výpis o publikaci