Monitoring Candida parapsilosis and Staphylococcus epidermidis
Biofilms by a Combination of Scanning Electron Microscopy and
Raman Spectroscopy

J 2018

Monitoring Candida parapsilosis and Staphylococcus epidermidis Biofilms by a Combination of Scanning Electron Microscopy and Raman Spectroscopy

HRUBANOVA, Kamila, Vladislav KRZYZANEK, Jana NEBESAROVA, Filip RŮŽIČKA, Zdenek PILAT et. al.

Základní údaje

Originální název

Monitoring Candida parapsilosis and Staphylococcus epidermidis Biofilms by a Combination of Scanning Electron Microscopy and Raman Spectroscopy

Autoři

HRUBANOVA, Kamila (203 Česká republika), Vladislav KRZYZANEK (203 Česká republika), Jana NEBESAROVA (203 Česká republika), Filip RŮŽIČKA (203 Česká republika, domácí), Zdenek PILAT (203 Česká republika) a Ota SAMEK (203 Česká republika, garant)

Vydání

Sensors, Basel, MDPI AG, 2018, 1424-8220

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.031

Kód RIV

RIV/00216224:14110/18:00105966

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.3390/s18124089

UT WoS

000454817100008

Klíčová slova anglicky

Raman spectroscopy; biofilm; sample preparation; scanning electron microscopy; cryo-SEM

Štítky

14110113, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 2. 2019 16:16, Soňa Böhmová

Anotace

V originále

The biofilm-forming microbial species Candida parapsilosis and Staphylococcus epidermidis have been recently linked to serious infections associated with implanted medical devices. We studied microbial biofilms by high resolution scanning electron microscopy (SEM), which allowed us to visualize the biofilm structure, including the distribution of cells inside the extracellular matrix and the areas of surface adhesion. We compared classical SEM (chemically fixed samples) with cryogenic SEM, which employs physical sample preparation based on plunging the sample into various liquid cryogens, as well as high-pressure freezing (HPF). For imaging the biofilm interior, we applied the freeze-fracture technique. In this study, we show that the different means of sample preparation have a fundamental influence on the observed biofilm structure. We complemented the SEM observations with Raman spectroscopic analysis, which allowed us to assess the time-dependent chemical composition changes of the biofilm in vivo. We identified the individual spectral peaks of the biomolecules present in the biofilm and we employed principal component analysis (PCA) to follow the temporal development of the chemical composition.

Návaznosti

LM2015062, projekt VaV

Název: Národní infrastruktura pro biologické a medicínské zobrazování

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, National research infrastructure for biological and medical imaging

NV16-29916A, projekt VaV

Název: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu

NV16-31593A, projekt VaV

Název: Polymikrobiální biofilm jako komplexní mikrobiom v rámci léčby nozokomiálních infekcí

Citovat

HRUBANOVA, Kamila, Vladislav KRZYZANEK, Jana NEBESAROVA, Filip RŮŽIČKA, Zdenek PILAT a Ota SAMEK. Monitoring Candida parapsilosis and Staphylococcus epidermidis Biofilms by a Combination of Scanning Electron Microscopy and Raman Spectroscopy. Sensors. Basel: MDPI AG, 2018, roč. 18, č. 12, s. 1-19. ISSN 1424-8220. Dostupné z: https://dx.doi.org/10.3390/s18124089.

@article{1491959,
   author = {Hrubanova, Kamila and Krzyzanek, Vladislav and Nebesarova, Jana and Růžička, Filip and Pilat, Zdenek and Samek, Ota},
   article_location = {Basel},
   article_number = {12},
   doi = {http://dx.doi.org/10.3390/s18124089},
   keywords = {Raman spectroscopy; biofilm; sample preparation; scanning electron microscopy; cryo-SEM},
   language = {eng},
   issn = {1424-8220},
   journal = {Sensors},
   title = {Monitoring Candida parapsilosis and Staphylococcus epidermidis Biofilms by a Combination of Scanning Electron Microscopy and Raman Spectroscopy},
   volume = {18},
   year = {2018}
}

TY  - JOUR
ID  - 1491959
AU  - Hrubanova, Kamila - Krzyzanek, Vladislav - Nebesarova, Jana - Růžička, Filip - Pilat, Zdenek - Samek, Ota
PY  - 2018
TI  - Monitoring Candida parapsilosis and Staphylococcus epidermidis Biofilms by a Combination of Scanning Electron Microscopy and Raman Spectroscopy
JF  - Sensors
VL  - 18
IS  - 12
SP  - 1-19
EP  - 1-19
PB  - MDPI AG
SN  - 14248220
KW  - Raman spectroscopy
KW  - biofilm
KW  - sample preparation
KW  - scanning electron microscopy
KW  - cryo-SEM
N2  - The biofilm-forming microbial species Candida parapsilosis and Staphylococcus epidermidis have been recently linked to serious infections associated with implanted medical devices. We studied microbial biofilms by high resolution scanning electron microscopy (SEM), which allowed us to visualize the biofilm structure, including the distribution of cells inside the extracellular matrix and the areas of surface adhesion. We compared classical SEM (chemically fixed samples) with cryogenic SEM, which employs physical sample preparation based on plunging the sample into various liquid cryogens, as well as high-pressure freezing (HPF). For imaging the biofilm interior, we applied the freeze-fracture technique. In this study, we show that the different means of sample preparation have a fundamental influence on the observed biofilm structure. We complemented the SEM observations with Raman spectroscopic analysis, which allowed us to assess the time-dependent chemical composition changes of the biofilm in vivo. We identified the individual spectral peaks of the biomolecules present in the biofilm and we employed principal component analysis (PCA) to follow the temporal development of the chemical composition.
ER  -

HRUBANOVA, Kamila, Vladislav KRZYZANEK, Jana NEBESAROVA, Filip RŮŽIČKA, Zdenek PILAT a Ota SAMEK. Monitoring Candida parapsilosis and Staphylococcus epidermidis Biofilms by a Combination of Scanning Electron Microscopy and Raman Spectroscopy. \textit{Sensors}. Basel: MDPI AG, 2018, roč.~18, č.~12, s.~1-19. ISSN~1424-8220. Dostupné z: https://dx.doi.org/10.3390/s18124089.

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