Electrophoretic techniques for purification, separation and
detection of Kayvirus with subsequent control by
matrix-assisted laser desorption/ionization time-of-flight mass
spectrometry and microbiological methods

J 2018

Electrophoretic techniques for purification, separation and detection of Kayvirus with subsequent control by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and microbiological methods

HORKÁ, Marie, Dana ŠTVERÁKOVÁ, Jiří ŠALPLACHTA, Karel ŠLAIS, Marta ŠIBOROVÁ et. al.

Základní údaje

Originální název

Electrophoretic techniques for purification, separation and detection of Kayvirus with subsequent control by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and microbiological methods

Autoři

HORKÁ, Marie (203 Česká republika, garant), Dana ŠTVERÁKOVÁ (203 Česká republika, domácí), Jiří ŠALPLACHTA (203 Česká republika), Karel ŠLAIS (203 Česká republika), Marta ŠIBOROVÁ (203 Česká republika, domácí), Filip RŮŽIČKA (203 Česká republika, domácí) a Roman PANTŮČEK (203 Česká republika, domácí)

Vydání

Journal of Chromatography A, Amsterdam, Netherlands, Elsevier, 2018, 0021-9673

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10609 Biochemical research methods

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.858

Kód RIV

RIV/00216224:14310/18:00105970

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.chroma.2018.07.078

UT WoS

000445982400018

Klíčová slova anglicky

Capillary electrophoretic methods; Preparative IEF; Purification; Pre-concentration; Separation; Kayvirus; Staphylococcus aureus; MALDI-TOF MS

Štítky

14110113, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 10. 2020 10:42, prof. RNDr. Roman Pantůček, Ph.D.

Anotace

V originále

The bacteriophage K1/420 is a member of genus Kayvirus that was extensively studied as an alternative treatment to combat bacterial infections caused by antibiotic-resistant Staphylococcus aureus strains. Despite the promise of phage therapy, the development of clinical applications of phages is facing regulatory and technical hurdles before it can receive acceptance in the Western World. Suitable simple and accurate diagnostic techniques to control the quality of the phage, which would satisfy the requirements of regulatory authorities are still being discussed. Here, we present the conditions for the simultaneous separation and detection of phage K1/420 and S. aureus by CZE and by CIEF were found, and the phage isoelectric point was determined to be 3.6. After removing the cell debris, the phage was successfully purified from the crude phage lysate and pre-concentrated by preparative isoelectric focusing. Its zone was localized by the positions of colored pI markers in the cellulose bed. The phage from the harvested zone had a decreased ability to infect its host. However, it was suitable for its separation, detection and identification by capillary electrophoretic methods, MALDI-TOF MS and electron microscopy. (C) 2018 Elsevier B.V. All rights reserved.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

NV16-29916A, projekt VaV

Název: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu

VI20172020069, projekt VaV

Název: Vývoj moderních instrumentálních metod pro rychlou detekci a identifikaci vybraných B-agens a toxinů (Akronym: VMIMRDIBT)

Investor: Ministerstvo vnitra ČR, Vývoj moderních instrumentálních metod pro rychlou detekci a identifikaci vybraných B-agens a toxinů

Citovat

HORKÁ, Marie, Dana ŠTVERÁKOVÁ, Jiří ŠALPLACHTA, Karel ŠLAIS, Marta ŠIBOROVÁ, Filip RŮŽIČKA a Roman PANTŮČEK. Electrophoretic techniques for purification, separation and detection of Kayvirus with subsequent control by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and microbiological methods. Journal of Chromatography A. Amsterdam, Netherlands: Elsevier, 2018, roč. 1570, OCT, s. 155-163. ISSN 0021-9673. Dostupné z: https://dx.doi.org/10.1016/j.chroma.2018.07.078.

@article{1491997,
   author = {Horká, Marie and Štveráková, Dana and Šalplachta, Jiří and Šlais, Karel and Šiborová, Marta and Růžička, Filip and Pantůček, Roman},
   article_location = {Amsterdam, Netherlands},
   article_number = {OCT},
   doi = {http://dx.doi.org/10.1016/j.chroma.2018.07.078},
   keywords = {Capillary electrophoretic methods; Preparative IEF; Purification; Pre-concentration; Separation; Kayvirus; Staphylococcus aureus; MALDI-TOF MS},
   language = {eng},
   issn = {0021-9673},
   journal = {Journal of Chromatography A},
   title = {Electrophoretic techniques for purification, separation and detection of Kayvirus with subsequent control by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and microbiological methods},
   url = {https://www.sciencedirect.com/science/article/pii/S0021967318309658},
   volume = {1570},
   year = {2018}
}

TY  - JOUR
ID  - 1491997
AU  - Horká, Marie - Štveráková, Dana - Šalplachta, Jiří - Šlais, Karel - Šiborová, Marta - Růžička, Filip - Pantůček, Roman
PY  - 2018
TI  - Electrophoretic techniques for purification, separation and detection of Kayvirus with subsequent control by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and microbiological methods
JF  - Journal of Chromatography A
VL  - 1570
IS  - OCT
SP  - 155-163
EP  - 155-163
PB  - Elsevier
SN  - 00219673
KW  - Capillary electrophoretic methods
KW  - Preparative IEF
KW  - Purification
KW  - Pre-concentration
KW  - Separation
KW  - Kayvirus
KW  - Staphylococcus aureus
KW  - MALDI-TOF MS
UR  - https://www.sciencedirect.com/science/article/pii/S0021967318309658
L2  - https://www.sciencedirect.com/science/article/pii/S0021967318309658
N2  - The bacteriophage K1/420 is a member of genus Kayvirus that was extensively studied as an alternative treatment to combat bacterial infections caused by antibiotic-resistant Staphylococcus aureus strains. Despite the promise of phage therapy, the development of clinical applications of phages is facing regulatory and technical hurdles before it can receive acceptance in the Western World. Suitable simple and accurate diagnostic techniques to control the quality of the phage, which would satisfy the requirements of regulatory authorities are still being discussed. Here, we present the conditions for the simultaneous separation and detection of phage K1/420 and S. aureus by CZE and by CIEF were found, and the phage isoelectric point was determined to be 3.6. After removing the cell debris, the phage was successfully purified from the crude phage lysate and pre-concentrated by preparative isoelectric focusing. Its zone was localized by the positions of colored pI markers in the cellulose bed. The phage from the harvested zone had a decreased ability to infect its host. However, it was suitable for its separation, detection and identification by capillary electrophoretic methods, MALDI-TOF MS and electron microscopy. (C) 2018 Elsevier B.V. All rights reserved.
ER  -

HORKÁ, Marie, Dana ŠTVERÁKOVÁ, Jiří ŠALPLACHTA, Karel ŠLAIS, Marta ŠIBOROVÁ, Filip RŮŽIČKA a Roman PANTŮČEK. Electrophoretic techniques for purification, separation and detection of Kayvirus with subsequent control by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and microbiological methods. \textit{Journal of Chromatography A}. Amsterdam, Netherlands: Elsevier, 2018, roč.~1570, OCT, s.~155-163. ISSN~0021-9673. Dostupné z: https://dx.doi.org/10.1016/j.chroma.2018.07.078.

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