Non-invasive electromechanical cell-based biosensors for
improved investigation of 3D cardiac models

J 2019

Non-invasive electromechanical cell-based biosensors for improved investigation of 3D cardiac models

CALUORI, Guido, Jan PŘIBYL, Martin PEŠL, Šárka JELÍNKOVÁ, Vladimír ROTREKL et. al.

Základní údaje

Originální název

Non-invasive electromechanical cell-based biosensors for improved investigation of 3D cardiac models

Autoři

CALUORI, Guido (380 Itálie, domácí), Jan PŘIBYL (203 Česká republika, domácí), Martin PEŠL (203 Česká republika, domácí), Šárka JELÍNKOVÁ (203 Česká republika, domácí), Vladimír ROTREKL (203 Česká republika, domácí), Petr SKLÁDAL (203 Česká republika, garant, domácí) a Roberto RAITERI (380 Itálie)

Vydání

Biosensors & bioelectronics : the international journal for the professional involved with research, technology and applications of biosensors and related devices, Elsevier Science, 2019, 0956-5663

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30404 Biomaterials

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 10.257

Kód RIV

RIV/00216224:14740/19:00107272

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.bios.2018.10.021

UT WoS

000451935500016

Klíčová slova anglicky

Excitation-contraction coupling; Human pluripotent stem cells; Cardiomyocytes; Atomic force microscopy; Microelectrode array; Drug testing

Štítky

CF NANO, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 10. 2024 12:23, Mgr. Adéla Pešková

Anotace

V originále

Cardiomyocytes (CM) placed on microelectrode array (MEA) were simultaneously probed with cantilever from atomic force microscope (AFM) system. This electric,nanomechanical combination in real time recorded beating force of the CMs cluster and the triggering electric events. Such "organ-on-a-chip" represents a tool for drug development and disease modeling. The human pluripotent stem cells included the WT embryonic line CCTL14 and the induced dystrophin deficient line reprogrammed from fibroblasts of a patient affected by Duchenne Muscular Dystrophy (DMD, complete loss of dystrophin expression). Both were differentiated to CMs and employed with the AFM/MEA platform for diseased CMs’ drug response testing and DMD characterization. The dependence of cardiac parameters on extracellular Ca2+ was studied. The differential evaluation explained the observed effects despite variability of biological samples. The beta-adrenergic stimulation (isoproterenol) and antagonist trials (verapamil) addressed ionotropic and chronotropic cell line-dependent features. For the first time, a distinctive beating-force relation for DMD CMs was measured on the 3D cardiac in vitro model.

Návaznosti

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

LM2015043, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii (Akronym: CIISB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Infrastructure for Integrative Structural Biology

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

MUNI/A/1087/2018, interní kód MU

Název: Molekulární a buněčná biologie pro biomedicínské vědy

Investor: Masarykova univerzita, Molekulární a buněčná biologie pro biomedicínské vědy, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

CALUORI, Guido, Jan PŘIBYL, Martin PEŠL, Šárka JELÍNKOVÁ, Vladimír ROTREKL, Petr SKLÁDAL a Roberto RAITERI. Non-invasive electromechanical cell-based biosensors for improved investigation of 3D cardiac models. Biosensors & bioelectronics : the international journal for the professional involved with research, technology and applications of biosensors and related devices. Elsevier Science, 2019, roč. 124, JAN 15 2019, s. 129-135. ISSN 0956-5663. Dostupné z: https://dx.doi.org/10.1016/j.bios.2018.10.021.

@article{1498076,
   author = {Caluori, Guido and Přibyl, Jan and Pešl, Martin and Jelínková, Šárka and Rotrekl, Vladimír and Skládal, Petr and Raiteri, Roberto},
   article_number = {JAN 15 2019},
   doi = {http://dx.doi.org/10.1016/j.bios.2018.10.021},
   keywords = {Excitation-contraction coupling; Human pluripotent stem cells; Cardiomyocytes; Atomic force microscopy; Microelectrode array; Drug testing},
   language = {eng},
   issn = {0956-5663},
   journal = {Biosensors & bioelectronics : the international journal for the professional involved with research, technology and applications of biosensors and related devices},
   title = {Non-invasive electromechanical cell-based biosensors for improved investigation of 3D cardiac models},
   url = {https://www.sciencedirect.com/science/article/pii/S0956566318308315?via%3Dihub},
   volume = {124},
   year = {2019}
}

TY  - JOUR
ID  - 1498076
AU  - Caluori, Guido - Přibyl, Jan - Pešl, Martin - Jelínková, Šárka - Rotrekl, Vladimír - Skládal, Petr - Raiteri, Roberto
PY  - 2019
TI  - Non-invasive electromechanical cell-based biosensors for improved investigation of 3D cardiac models
JF  - Biosensors & bioelectronics : the international journal for the professional involved with research, technology and applications of biosensors and related devices
VL  - 124
IS  - JAN 15 2019
SP  - 129-135
EP  - 129-135
PB  - Elsevier Science
SN  - 09565663
KW  - Excitation-contraction coupling
KW  - Human pluripotent stem cells
KW  - Cardiomyocytes
KW  - Atomic force microscopy
KW  - Microelectrode array
KW  - Drug testing
UR  - https://www.sciencedirect.com/science/article/pii/S0956566318308315?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S0956566318308315?via%3Dihub
N2  - Cardiomyocytes (CM) placed on microelectrode array (MEA) were simultaneously probed with cantilever from atomic force microscope (AFM) system. This electric,nanomechanical combination in real time recorded beating force of the CMs cluster and the triggering electric events. Such "organ-on-a-chip" represents a tool for drug development and disease modeling. The human pluripotent stem cells included the WT embryonic line CCTL14 and the induced dystrophin deficient line reprogrammed from fibroblasts of a patient affected by Duchenne Muscular Dystrophy (DMD, complete loss of dystrophin expression). Both were differentiated to CMs and employed with the AFM/MEA platform for diseased CMs’ drug response testing and DMD characterization. The dependence of cardiac parameters on extracellular Ca2+ was studied. The differential evaluation explained the observed effects despite variability of biological samples. The beta-adrenergic stimulation (isoproterenol) and antagonist trials (verapamil) addressed ionotropic and chronotropic cell line-dependent features. For the first time, a distinctive beating-force relation for DMD CMs was measured on the 3D cardiac in vitro model.
ER  -

CALUORI, Guido, Jan PŘIBYL, Martin PEŠL, Šárka JELÍNKOVÁ, Vladimír ROTREKL, Petr SKLÁDAL a Roberto RAITERI. Non-invasive electromechanical cell-based biosensors for improved investigation of 3D cardiac models. \textit{Biosensors \&{} bioelectronics : the international journal for the professional involved with research, technology and applications of biosensors and related devices}. Elsevier Science, 2019, roč.~124, JAN 15 2019, s.~129-135. ISSN~0956-5663. Dostupné z: https://dx.doi.org/10.1016/j.bios.2018.10.021.

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