Biomechanical Characterization of Human Pluripotent Stem
Cell-Derived Cardiomyocytes by Use of Atomic Force Microscopy

C 2019

Biomechanical Characterization of Human Pluripotent Stem Cell-Derived Cardiomyocytes by Use of Atomic Force Microscopy

PŘIBYL, Jan, Martin PEŠL, Guido CALUORI, Ivana AĆIMOVIĆ, Šárka JELÍNKOVÁ et. al.

Základní údaje

Originální název

Biomechanical Characterization of Human Pluripotent Stem Cell-Derived Cardiomyocytes by Use of Atomic Force Microscopy

Autoři

PŘIBYL, Jan (203 Česká republika, domácí), Martin PEŠL (203 Česká republika, garant, domácí), Guido CALUORI (203 Česká republika), Ivana AĆIMOVIĆ (380 Itálie, domácí), Šárka JELÍNKOVÁ (203 Česká republika, domácí), Petr DVOŘÁK (203 Česká republika, domácí), Petr SKLÁDAL (203 Česká republika, domácí) a Vladimír ROTREKL (203 Česká republika, domácí)

Vydání

New York, NY, Atomic Force Microscopy, od s. 343-353, 11 s. Methods in Molecular Biology, volume 1886, 2019

Nakladatel

Springer

Další údaje

Jazyk

angličtina

Typ výsledku

Kapitola resp. kapitoly v odborné knize

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Odkazy

URL

Kód RIV

RIV/00216224:14110/19:00107274

Organizační jednotka

Lékařská fakulta

ISBN

978-1-4939-8893-8

DOI

http://dx.doi.org/10.1007/978-1-4939-8894-5_20

UT WoS

000683074500021

Klíčová slova anglicky

Atomic force microscopy; Biomechanical characterization; Human stem cell; Cardiomyocyte contraction; Drug testing

Štítky

rivok, topvydavatel

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 1. 2022 12:49, Mgr. Marie Šípková, DiS.

Anotace

V originále

Atomic force microscopy (AFM) is not only a high-resolution imaging technique but also a sensitive tool able to study biomechanical properties of bio-samples (biomolecules, cells) in native conditions—i.e., in buffered solutions (culturing media) and stable temperature (mostly 37 °C). Micromechanical transducers (cantilevers) are often used to map surface stiffness distribution, adhesion forces, and viscoelastic parameters of living cells; however, they can also be used to monitor time course of cardiomyocytes contraction dynamics (e.g. beating rate, relaxation time), together with other biomechanical properties. Here we describe the construction of an AFM-based biosensor setup designed to study the biomechanical properties of cardiomyocyte clusters, through the use of standard uncoated silicon nitride cantilevers. Force-time curves (mechanocardiograms, MCG) are recorded continuously in real time and in the presence of cardiomyocyte-contraction affecting drugs (e.g., isoproterenol, metoprolol) in the medium, under physiological conditions. The average value of contraction force and the beat rate, as basic biomechanical parameters, represent pharmacological indicators of different phenotype features. Robustness, low computational requirements, and optimal spatial sensitivity (detection limit 200 pN, respectively 20 nm displacement) are the main advantages of the presented method.

Návaznosti

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

MUNI/A/1010/2016, interní kód MU

Název: Efekt elektroporační ablace na lidské srdeční buňky

Investor: Masarykova univerzita, Efekt elektroporační ablace na lidské srdeční buňky, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

PŘIBYL, Jan, Martin PEŠL, Guido CALUORI, Ivana AĆIMOVIĆ, Šárka JELÍNKOVÁ, Petr DVOŘÁK, Petr SKLÁDAL a Vladimír ROTREKL. Biomechanical Characterization of Human Pluripotent Stem Cell-Derived Cardiomyocytes by Use of Atomic Force Microscopy. In Nuno C. Santos; Filomena A. Carvalho. Atomic Force Microscopy. New York, NY: Springer, 2019, s. 343-353. Methods in Molecular Biology, volume 1886. ISBN 978-1-4939-8893-8. Dostupné z: https://dx.doi.org/10.1007/978-1-4939-8894-5_20.

@inbook{1498119,
   author = {Přibyl, Jan and Pešl, Martin and Caluori, Guido and Aćimović, Ivana and Jelínková, Šárka and Dvořák, Petr and Skládal, Petr and Rotrekl, Vladimír},
   address = {New York, NY},
   booktitle = {Atomic Force Microscopy},
   doi = {http://dx.doi.org/10.1007/978-1-4939-8894-5_20},
   editor = {Nuno C. Santos; Filomena A. Carvalho},
   keywords = {Atomic force microscopy; Biomechanical characterization; Human stem cell; Cardiomyocyte contraction; Drug testing},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {New York, NY},
   isbn = {978-1-4939-8893-8},
   pages = {343-353},
   publisher = {Springer},
   title = {Biomechanical Characterization of Human Pluripotent Stem Cell-Derived Cardiomyocytes by Use of Atomic Force Microscopy},
   url = {https://link.springer.com/protocol/10.1007%2F978-1-4939-8894-5_20},
   year = {2019}
}

TY  - CHAP
ID  - 1498119
AU  - Přibyl, Jan - Pešl, Martin - Caluori, Guido - Aćimović, Ivana - Jelínková, Šárka - Dvořák, Petr - Skládal, Petr - Rotrekl, Vladimír
PY  - 2019
TI  - Biomechanical Characterization of Human Pluripotent Stem Cell-Derived Cardiomyocytes by Use of Atomic Force Microscopy
VL  - Methods in Molecular Biology, volume 1886
PB  - Springer
CY  - New York, NY
SN  - 9781493988938
KW  - Atomic force microscopy
KW  - Biomechanical characterization
KW  - Human stem cell
KW  - Cardiomyocyte contraction
KW  - Drug testing
UR  - https://link.springer.com/protocol/10.1007%2F978-1-4939-8894-5_20
L2  - https://link.springer.com/protocol/10.1007%2F978-1-4939-8894-5_20
N2  - Atomic force microscopy (AFM) is not only a high-resolution imaging technique but also a sensitive tool able to study biomechanical properties of bio-samples (biomolecules, cells) in native conditions—i.e., in buffered solutions (culturing media) and stable temperature (mostly 37 °C). Micromechanical transducers (cantilevers) are often used to map surface stiffness distribution, adhesion forces, and viscoelastic parameters of living cells; however, they can also be used to monitor time course of cardiomyocytes contraction dynamics (e.g. beating rate, relaxation time), together with other biomechanical properties. Here we describe the construction of an AFM-based biosensor setup designed to study the biomechanical properties of cardiomyocyte clusters, through the use of standard uncoated silicon nitride cantilevers. Force-time curves (mechanocardiograms, MCG) are recorded continuously in real time and in the presence of cardiomyocyte-contraction affecting drugs (e.g., isoproterenol, metoprolol) in the medium, under physiological conditions. The average value of contraction force and the beat rate, as basic biomechanical parameters, represent pharmacological indicators of different phenotype features. Robustness, low computational requirements, and optimal spatial sensitivity (detection limit 200 pN, respectively 20 nm displacement) are the main advantages of the presented method.
ER  -

PŘIBYL, Jan, Martin PEŠL, Guido CALUORI, Ivana A$\backslash$'CIMOVI$\backslash$'C, Šárka JELÍNKOVÁ, Petr DVOŘÁK, Petr SKLÁDAL a Vladimír ROTREKL. Biomechanical Characterization of Human Pluripotent Stem Cell-Derived Cardiomyocytes by Use of Atomic Force Microscopy. In Nuno C. Santos; Filomena A. Carvalho. \textit{Atomic Force Microscopy}. New York, NY: Springer, 2019, s.~343-353. Methods in Molecular Biology, volume 1886. ISBN~978-1-4939-8893-8. Dostupné z: https://dx.doi.org/10.1007/978-1-4939-8894-5\_{}20.

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