Future prospects of structural studies to advance our
understanding of phage biology

J 2019

Future prospects of structural studies to advance our understanding of phage biology

BÁRDY, Pavol; Dominik HREBÍK; Roman PANTŮČEK a Pavel PLEVKA

Základní údaje

Originální název

Future prospects of structural studies to advance our understanding of phage biology

Autoři

BÁRDY, Pavol; Dominik HREBÍK; Roman PANTŮČEK a Pavel PLEVKA

Vydání

Microbiology Australia, Clayton, VIC, Australia, CSIRO Publishing, 2019, 1324-4272

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10607 Virology

Stát vydavatele

Austrálie

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/19:00107302

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

bacteriophage; structural biology; biofilm; phage therapy; cryo-electron microscopy

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 3. 3. 2020 15:51, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Bacteriophages, being the most abundant biological entities on the Earth, play a major role in regulating populations of bacteria and thus influence the evolution and stability of ecosystems. Phage infections of pathogenic bacteria can both exacerbate and alleviate the severity of the disease. The structural characterisations of phage particles and individual proteins have enabled the understanding of many aspects of phage biology. Due to methodological limitations, most of the structures were determined from purified samples in vitro. However, studies performed outside the cellular context cannot capture the complex and dynamic interactions of the macromolecules that are required for their biological functions. Current developments in structural biology, in particular cryo-electron microscopy, allow in situ high-resolution studies of phage-infected cells. Here we discuss open questions in phage biology that could be addressed by structural biology techniques and their potential to enable the use of tailed phages in industrial applications and human therapy.

Návaznosti

GA18-13064S, projekt VaV

Název: Analýza interakcí mezi polyvalentním terapeutickým fágem druhu Twort-like a jeho hostitelem Staphylococcus aureus

Investor: Grantová agentura ČR, Analýza interakcí mezi polyvalentním terapeutickým fágem druhu Twort-like a jeho hostitelem Staphylococcus aureus

GA18-17810S, projekt VaV

Název: Strukturní analýza replikace fága v buňkách zlatého stafylokoka

Investor: Grantová agentura ČR, In vivo strukturní analýza replikace fága infikujícího zlatého stafylokoka

NV16-29916A, projekt VaV

Název: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu

3041, interní kód MU

Název: Structural studies of human and animal pathogens from the order Picornavirales

Investor: EMBO (European Molecular Biology Organization), Structural studies of human and animal pathogens from the order Picornavirales

Přiložené soubory

MA19009_FINAL.pdf

Požádat o autorskou verzi souboru

Citovat

BÁRDY, Pavol; Dominik HREBÍK; Roman PANTŮČEK a Pavel PLEVKA. Future prospects of structural studies to advance our understanding of phage biology. Microbiology Australia. Clayton, VIC, Australia: CSIRO Publishing, 2019, roč. 40, č. 1, s. 42-46. ISSN 1324-4272. Dostupné z: https://doi.org/10.1071/MA19009.

@article{1502696,
   author = {Bárdy, Pavol and Hrebík, Dominik and Pantůček, Roman and Plevka, Pavel},
   article_location = {Clayton, VIC, Australia},
   article_number = {1},
   doi = {https://doi.org/10.1071/MA19009},
   keywords = {bacteriophage; structural biology; biofilm; phage therapy; cryo-electron microscopy},
   language = {eng},
   issn = {1324-4272},
   journal = {Microbiology Australia},
   title = {Future prospects of structural studies to advance our understanding of phage biology},
   url = {http://microbiology.publish.csiro.au/?paper=MA19009},
   volume = {40},
   year = {2019}
}

TY  - JOUR
ID  - 1502696
AU  - Bárdy, Pavol - Hrebík, Dominik - Pantůček, Roman - Plevka, Pavel
PY  - 2019
TI  - Future prospects of structural studies to advance our understanding of phage biology
JF  - Microbiology Australia
VL  - 40
IS  - 1
SP  - 42-46
EP  - 42-46
PB  - CSIRO Publishing
SN  - 13244272
KW  - bacteriophage
KW  - structural biology
KW  - biofilm
KW  - phage therapy
KW  - cryo-electron microscopy
UR  - http://microbiology.publish.csiro.au/?paper=MA19009
L2  - http://microbiology.publish.csiro.au/?paper=MA19009
N2  - Bacteriophages, being the most abundant biological entities on the Earth, play a major role in regulating populations of bacteria and thus influence the evolution and stability of ecosystems. Phage infections of pathogenic bacteria can both exacerbate and alleviate the severity of the disease. The structural characterisations of phage particles and individual proteins have enabled the understanding of many aspects of phage biology. Due to methodological limitations, most of the structures were determined from purified samples in vitro. However, studies performed outside the cellular context cannot capture the complex and dynamic interactions of the macromolecules that are required for their biological functions. Current developments in structural biology, in particular cryo-electron microscopy, allow in situ high-resolution studies of phage-infected cells. Here we discuss open questions in phage biology that could be addressed by structural biology techniques and their potential to enable the use of tailed phages in industrial applications and human therapy.
ER  -

BÁRDY, Pavol; Dominik HREBÍK; Roman PANTŮČEK a Pavel PLEVKA. Future prospects of structural studies to advance our understanding of phage biology. \textit{Microbiology Australia}. Clayton, VIC, Australia: CSIRO Publishing, 2019, roč.~40, č.~1, s.~42-46. ISSN~1324-4272. Dostupné z: https://doi.org/10.1071/MA19009.

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