Advances in the cellular structural biology of nucleic acids

GIASSA, Ilektra-Chara, Jan RYNEŠ, T. FESSL, Silvie TRANTÍRKOVÁ and Lukáš TRANTÍREK. Advances in the cellular structural biology of nucleic acids. FEBS LETTERS. HOBOKEN: WILEY, 2018, vol. 592, No 12, p. 1997-2011. ISSN 1873-3468. Available from: https://dx.doi.org/10.1002/1873-3468.13054.

Basic information

• Original name: Advances in the cellular structural biology of nucleic acids
• Authors: GIASSA, Ilektra-Chara (300 Greece, belonging to the institution), Jan RYNEŠ (203 Czech Republic, belonging to the institution), T. FESSL (203 Czech Republic), Silvie TRANTÍRKOVÁ (203 Czech Republic, belonging to the institution) and Lukáš TRANTÍREK (203 Czech Republic, guarantor, belonging to the institution).
• Edition: FEBS LETTERS, HOBOKEN, WILEY, 2018, 1873-3468.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 2.675
• RIV identification code: RIV/00216224:14740/18:00101748
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1002/1873-3468.13054
• UT WoS: 000436109600009
• Keywords in English: DNA; in-cell chemical probing; in-cell EPR; in-cell NMR; in-cell single-molecule FRET; RNA
• Tags: rivok
• Tags: International impact, Reviewed

Abstract

Conventional biophysical and chemical biology approaches for delineating relationships between the structure and biological function of nucleic acids (NAs) abstract NAs from their native biological context. However, cumulative experimental observations have revealed that the structure, dynamics and interactions of NAs might be strongly influenced by a broad spectrum of specific and nonspecific physical-chemical environmental factors. This consideration has recently sparked interest in the development of novel tools for structural characterization of NAs in the native cellular context. Here, we review the individual methods currently being employed for structural characterization of NA structure in a native cellular environment with a focus on recent advances and developments in the emerging fields of in-cell NMR and electron paramagnetic resonance spectroscopy and in-cell single-molecule FRET of NAs.

Links

• GA16-10504S, research and development project: Name: Charakterizace struktury nukleových kyselin v komplexním prostředí živých buněk pomocí vysoce rozlišené NMR spektroskopie

Investor: Czech Science Foundation

• NV15-33232A, research and development project: Name: Identifikace nových možností léčby achondroplásie prostřednictvím analýzy interakce FGFR3 a adaptérového proteinu Frs2
• NV15-34405A, research and development project: Name: Identifikace nových možností léčby chronické myeloidní leukémie pomocí systematické analýzy interaktomu proteinu BCR-ABL
• 653706, interní kód MU: Name: iNEXT - Infrastructure for NMR, EM and X-ray crystallography for translational research (Acronym: iNEXT)

Investor: European Union, iNEXT - Infrastructure for NMR, EM and X-ray crystallography for translational research, RI Research Infrastructures (Excellent Science)