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@article{1503339,
author = {Pilat, Z. and Kizovsky, M. and Jezek, J. and Kratky, S. and Sobota, J. and Šiler, Martin and Samek, O. and Buryška, Tomáš and Vaňáček, Pavel and Damborský, Jiří and Prokop, Zbyněk and Zemanek, P.},
article_location = {Basel},
article_number = {10},
doi = {http://dx.doi.org/10.3390/s18103212},
keywords = {surface enhanced Raman spectroscopy; microfluidics; Klarite 312; chloroalkane; 1; 2; 3-trichloropropane},
language = {eng},
issn = {1424-8220},
journal = {Sensors},
title = {Detection of Chloroalkanes by Surface-Enhanced Raman Spectroscopy in Microfluidic Chips},
url = {https://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2018/09/sensors-18-03212.pdf},
volume = {18},
year = {2018}
}
TY - JOUR
ID - 1503339
AU - Pilat, Z. - Kizovsky, M. - Jezek, J. - Kratky, S. - Sobota, J. - Šiler, Martin - Samek, O. - Buryška, Tomáš - Vaňáček, Pavel - Damborský, Jiří - Prokop, Zbyněk - Zemanek, P.
PY - 2018
TI - Detection of Chloroalkanes by Surface-Enhanced Raman Spectroscopy in Microfluidic Chips
JF - Sensors
VL - 18
IS - 10
SP - 1-18
EP - 1-18
PB - MDPI AG
SN - 14248220
KW - surface enhanced Raman spectroscopy
KW - microfluidics
KW - Klarite 312
KW - chloroalkane
KW - 1
KW - 2
KW - 3-trichloropropane
UR - https://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2018/09/sensors-18-03212.pdf
N2 - Optofluidics, a research discipline combining optics with microfluidics, currently aspires to revolutionize the analysis of biological and chemical samples, e.g., for medicine, pharmacology, or molecular biology. In order to detect low concentrations of analytes in water, we have developed an optofluidic device containing a nanostructured substrate for surface enhanced Raman spectroscopy (SERS). The geometry of the gold surface allows localized plasmon oscillations to give rise to the SERS effect, in which the Raman spectral lines are intensified by the interaction of the plasmonic field with the electrons in the molecular bonds. The SERS substrate was enclosed in a microfluidic system, which allowed transport and precise mixing of the analyzed fluids, while preventing contamination or abrasion of the highly sensitive substrate. To illustrate its practical use, we employed the device for quantitative detection of persistent environmental pollutant 1,2,3-trichloropropane in water in submillimolar concentrations. The developed sensor allows fast and simple quantification of halogenated compounds and it will contribute towards the environmental monitoring and enzymology experiments with engineered haloalkane dehalogenase enzymes.
ER -
PILAT, Z., M. KIZOVSKY, J. JEZEK, S. KRATKY, J. SOBOTA, Martin ŠILER, O. SAMEK, Tomáš BURYŠKA, Pavel VAŇÁČEK, Jiří DAMBORSKÝ, Zbyněk PROKOP a P. ZEMANEK. Detection of Chloroalkanes by Surface-Enhanced Raman Spectroscopy in Microfluidic Chips. \textit{Sensors}. Basel: MDPI AG, 2018, roč.~18, č.~10, s.~1-18. ISSN~1424-8220. Dostupné z: https://dx.doi.org/10.3390/s18103212.
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