The Inhibitor Endosidin 4 Targets SEC7 Domain-Type ARF GTPase
Exchange Factors and Interferes with Subcellular Trafficking in
Eukaryotes

J 2018

The Inhibitor Endosidin 4 Targets SEC7 Domain-Type ARF GTPase Exchange Factors and Interferes with Subcellular Trafficking in Eukaryotes

KANIA, U.; Tomasz NODZYNSKI; Q. LU; G.R. HICKS; W. NERINCKX et. al.

Základní údaje

Originální název

The Inhibitor Endosidin 4 Targets SEC7 Domain-Type ARF GTPase Exchange Factors and Interferes with Subcellular Trafficking in Eukaryotes

Autoři

KANIA, U. (40 Rakousko); Tomasz NODZYNSKI (616 Polsko, garant, domácí); Q. LU (56 Belgie); G.R. HICKS (840 Spojené státy); W. NERINCKX (56 Belgie); K. MISHEV; F. PEUROIS (250 Francie); J. CHERFILS (250 Francie); R. DE RYCKE (56 Belgie); P. GRONES (56 Belgie); S. ROBERT (752 Švédsko); E. RUSSINOVA (56 Belgie) a J. FRIML (40 Rakousko)

Vydání

Plant Cell, ROCKVILLE, AMER SOC PLANT BIOLOGISTS, 2018, 1040-4651

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 8.631

Kód RIV

RIV/00216224:14740/18:00101767

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1105/tpc.18.00127

UT WoS

000450000500023

EID Scopus

2-s2.0-85056326063

Klíčová slova anglicky

GUANINE-NUCLEOTIDE-EXCHANGE; ADP-RIBOSYLATION FACTOR-1; AUXIN EFFLUX CARRIER; TRANS-GOLGI NETWORK; ENDOPLASMIC-RETICULUM; YEAST GOLGI; ARABIDOPSIS-THALIANA; ROOT GRAVITROPISM; BREFELDIN-A; INDUCED HAPLOINSUFFICIENCY

Štítky

CF CELLIM, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 11. 2024 14:09, Mgr. Eva Dubská

Anotace

V originále

The trafficking of subcellular cargos in eukaryotic cells crucially depends on vesicle budding, a process mediated by ARFGEFs (ADP-ribosylation factor guanine nucleotide exchange factors). In plants, ARF-GEFs play essential roles in endocytosis, vacuolar trafficking, recycling, secretion, and polar trafficking. Moreover, they are important for plant development, mainly through controlling the polar subcellular localization of PIN-FORMED transporters of the plant hormone auxin. Here, using a chemical genetics screen in Arabidopsis thaliana, we identified Endosidin 4 (ES4), an inhibitor of eukaryotic ARF-GEFs. ES4 acts similarly to and synergistically with the established ARF-GEF inhibitor Brefeldin A and has broad effects on intracellular trafficking, including endocytosis, exocytosis, and vacuolar targeting. Additionally, Arabidopsis and yeast (Saccharomyces cerevisiae) mutants defective in ARF-GEF show altered sensitivity to ES4. ES4 interferes with the activation-based membrane association of the ARF1 GTPases, but not of their mutant variants that are activated independently of ARF-GEF activity. Biochemical approaches and docking simulations confirmed that ES4 specifically targets the SEC7 domain-containing ARF-GEFs. These observations collectively identify ES4 as a chemical tool enabling the study of ARF-GEF-mediated processes, including ARF-GEF-mediated plant development.

Návaznosti

GA18-26981S, projekt VaV

Název: Genetické studie k objasnění molekulárního mechanizmu účinku strigolaktonů v kořeni

Investor: Grantová agentura ČR, Genetické studie k objasnění molekulárního mechanizmu účinku strigolaktonů v kořeni

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

90062, velká výzkumná infrastruktura

Název: Czech-BioImaging

Citovat

KANIA, U.; Tomasz NODZYNSKI; Q. LU; G.R. HICKS; W. NERINCKX; K. MISHEV; F. PEUROIS; J. CHERFILS; R. DE RYCKE; P. GRONES; S. ROBERT; E. RUSSINOVA a J. FRIML. The Inhibitor Endosidin 4 Targets SEC7 Domain-Type ARF GTPase Exchange Factors and Interferes with Subcellular Trafficking in Eukaryotes. Plant Cell. ROCKVILLE: AMER SOC PLANT BIOLOGISTS, 2018, roč. 30, č. 10, s. 2553-2572. ISSN 1040-4651. Dostupné z: https://doi.org/10.1105/tpc.18.00127.

@article{1504366,
   author = {Kania, U. and Nodzynski, Tomasz and Lu, Q. and Hicks, G.R. and Nerinckx, W. and Mishev, K. and Peurois, F. and Cherfils, J. and De Rycke, R. and Grones, P. and Robert, S. and Russinova, E. and Friml, J.},
   article_location = {ROCKVILLE},
   article_number = {10},
   doi = {https://doi.org/10.1105/tpc.18.00127},
   keywords = {GUANINE-NUCLEOTIDE-EXCHANGE; ADP-RIBOSYLATION FACTOR-1; AUXIN EFFLUX CARRIER; TRANS-GOLGI NETWORK; ENDOPLASMIC-RETICULUM; YEAST GOLGI; ARABIDOPSIS-THALIANA; ROOT GRAVITROPISM; BREFELDIN-A; INDUCED HAPLOINSUFFICIENCY},
   language = {eng},
   issn = {1040-4651},
   journal = {Plant Cell},
   title = {The Inhibitor Endosidin 4 Targets SEC7 Domain-Type ARF GTPase Exchange Factors and Interferes with Subcellular Trafficking in Eukaryotes},
   url = {https://academic.oup.com/plcell/article/30/10/2553/6099476?login=true},
   volume = {30},
   year = {2018}
}

TY  - JOUR
ID  - 1504366
AU  - Kania, U. - Nodzynski, Tomasz - Lu, Q. - Hicks, G.R. - Nerinckx, W. - Mishev, K. - Peurois, F. - Cherfils, J. - De Rycke, R. - Grones, P. - Robert, S. - Russinova, E. - Friml, J.
PY  - 2018
TI  - The Inhibitor Endosidin 4 Targets SEC7 Domain-Type ARF GTPase Exchange Factors and Interferes with Subcellular Trafficking in Eukaryotes
JF  - Plant Cell
VL  - 30
IS  - 10
SP  - 2553-2572
EP  - 2553-2572
PB  - AMER SOC PLANT BIOLOGISTS
SN  - 10404651
KW  - GUANINE-NUCLEOTIDE-EXCHANGE
KW  - ADP-RIBOSYLATION FACTOR-1
KW  - AUXIN EFFLUX CARRIER
KW  - TRANS-GOLGI NETWORK
KW  - ENDOPLASMIC-RETICULUM
KW  - YEAST GOLGI
KW  - ARABIDOPSIS-THALIANA
KW  - ROOT GRAVITROPISM
KW  - BREFELDIN-A
KW  - INDUCED HAPLOINSUFFICIENCY
UR  - https://academic.oup.com/plcell/article/30/10/2553/6099476?login=true
N2  - The trafficking of subcellular cargos in eukaryotic cells crucially depends on vesicle budding, a process mediated by ARFGEFs (ADP-ribosylation factor guanine nucleotide exchange factors). In plants, ARF-GEFs play essential roles in endocytosis, vacuolar trafficking, recycling, secretion, and polar trafficking. Moreover, they are important for plant development, mainly through controlling the polar subcellular localization of PIN-FORMED transporters of the plant hormone auxin. Here, using a chemical genetics screen in Arabidopsis thaliana, we identified Endosidin 4 (ES4), an inhibitor of eukaryotic ARF-GEFs. ES4 acts similarly to and synergistically with the established ARF-GEF inhibitor Brefeldin A and has broad effects on intracellular trafficking, including endocytosis, exocytosis, and vacuolar targeting. Additionally, Arabidopsis and yeast (Saccharomyces cerevisiae) mutants defective in ARF-GEF show altered sensitivity to ES4. ES4 interferes with the activation-based membrane association of the ARF1 GTPases, but not of their mutant variants that are activated independently of ARF-GEF activity. Biochemical approaches and docking simulations confirmed that ES4 specifically targets the SEC7 domain-containing ARF-GEFs. These observations collectively identify ES4 as a chemical tool enabling the study of ARF-GEF-mediated processes, including ARF-GEF-mediated plant development.
ER  -

KANIA, U.; Tomasz NODZYNSKI; Q. LU; G.R. HICKS; W. NERINCKX; K. MISHEV; F. PEUROIS; J. CHERFILS; R. DE RYCKE; P. GRONES; S. ROBERT; E. RUSSINOVA a J. FRIML. The Inhibitor Endosidin 4 Targets SEC7 Domain-Type ARF GTPase Exchange Factors and Interferes with Subcellular Trafficking in Eukaryotes. \textit{Plant Cell}. ROCKVILLE: AMER SOC PLANT BIOLOGISTS, 2018, roč.~30, č.~10, s.~2553-2572. ISSN~1040-4651. Dostupné z: https://doi.org/10.1105/tpc.18.00127.

Přehled o publikaci