REMŠÍK, Ján, Radek FEDR, J. NAVRATIL, Lucia BINÓ, Eva SLABÁKOVÁ, P. FABIAN, M. SVOBODA and Karel SOUČEK. Plasticity and intratumoural heterogeneity of cell surface antigen expression in breast cancer. British journal of cancer. London: Nature Publishing Group, 2018, vol. 118, No 6, p. 813-819. ISSN 0007-0920. Available from: https://dx.doi.org/10.1038/bjc.2017.497.
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Basic information
Original name Plasticity and intratumoural heterogeneity of cell surface antigen expression in breast cancer
Authors REMŠÍK, Ján (703 Slovakia, belonging to the institution), Radek FEDR (203 Czech Republic), J. NAVRATIL, Lucia BINÓ (703 Slovakia), Eva SLABÁKOVÁ (203 Czech Republic), P. FABIAN, M. SVOBODA and Karel SOUČEK (203 Czech Republic, guarantor, belonging to the institution).
Edition British journal of cancer, London, Nature Publishing Group, 2018, 0007-0920.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10601 Cell biology
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 5.416
RIV identification code RIV/00216224:14310/18:00106565
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1038/bjc.2017.497
UT WoS 000427945800020
Keywords in English breast cancer; epithelial-mesenchymal plasticity; intratumoural heterogeneity; CD9
Tags International impact, Reviewed
Changed by Changed by: Mgr. Michal Petr, učo 65024. Changed: 23/4/2024 14:27.
Abstract
Background: The intratumoural heterogeneity, often driven by epithelial-to-mesenchymal transition (EMT), significantly contributes to chemoresistance and disease progression in adenocarcinomas. Methods: We introduced a high-throughput screening platform to identify surface antigens that associate with epithelial-mesenchymal plasticity in well-defined pairs of epithelial cell lines and their mesenchymal counterparts. Using multicolour flow cytometry, we then analysed the expression of 10 most robustly changed antigens and identified a 10-molecule surface signature, in pan-cytokeratin-positive/EpCAM-positive and -negative fractions of dissociated breast tumours. Results: We found that surface CD9, CD29, CD49c, and integrin beta 5 are lost in breast cancer cells that underwent EMT in vivo. The tetraspanin family member CD9 was concordantly downregulated both in vitro and in vivo and associated with epithelial phenotype and favourable prognosis. Conclusions: We propose that overall landscape of 10-molecule surface signature expression reflects the epithelial-mesenchymal plasticity in breast cancer.
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