Viral discovery and diversity in trypanosomatid protozoa with a
focus on relatives of the human parasite Leishmania

J 2018

Viral discovery and diversity in trypanosomatid protozoa with a focus on relatives of the human parasite Leishmania

GRYBCHUK, D., N.S. AKOPYANTS, A.Y. KOSTYGOV, A. KONOVALOVAS, L.F. LYE et. al.

Basic information

Original name

Viral discovery and diversity in trypanosomatid protozoa with a focus on relatives of the human parasite Leishmania

Authors

GRYBCHUK, D. (203 Czech Republic), N.S. AKOPYANTS (840 United States of America), A.Y. KOSTYGOV (643 Russian Federation), A. KONOVALOVAS (440 Lithuania), L.F. LYE (840 United States of America), D.E. DOBSON (840 United States of America), H. ZANGGER (756 Switzerland), N. FASEL (756 Switzerland), A. BUTENKO (203 Czech Republic), A.O. FROLOV (643 Russian Federation), J. VOTYPKA (203 Czech Republic), C.M. DAVILA-LEVY (76 Brazil), P. KULICH (203 Czech Republic), Jana MORAVCOVÁ (203 Czech Republic, belonging to the institution), Pavel PLEVKA (203 Czech Republic, guarantor, belonging to the institution), I.B. ROGOZIN (840 United States of America), S. SERVA (440 Lithuania), J. LUKES (203 Czech Republic), S.M. BEVERLEY (840 United States of America) and V. YURCHENKO (643 Russian Federation)

Edition

Proceedings of the National Academy of Sciences of the United States of America, WASHINGTON, NATL ACAD SCIENCES, 2018, 0027-8424

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10607 Virology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 9.580

RIV identification code

RIV/00216224:14740/18:00106594

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1073/pnas.1717806115

UT WoS

000423091400021

Keywords in English

Trypanosomatidae; Bunyavirales; persistent virus infection; coinfection; coevolution

Abstract

V originále

Knowledge of viral diversity is expanding greatly, but many lineages remain underexplored. We surveyed RNA viruses in 52 cultured monoxenous relatives of the human parasite Leishmania (Crithidia and Leptomonas), as well as plant-infecting Phytomonas. Leptomonas pyrrhocoris was a hotbed for viral discovery, carrying a virus (Leptomonas pyrrhocoris ostravirus 1) with a highly divergent RNA-dependent RNA polymerase missed by conventional BLAST searches, an emergent clade of tombus-like viruses, and an example of viral endogenization. A deep-branching clade of trypanosomatid narnaviruses was found, notable as Leptomonas seymouri bearing Narna-like virus 1 (LepseyNLV1) have been reported in cultures recovered from patients with visceral leishmaniasis. A deep-branching trypanosomatid viral lineage showing strong affinities to bunyaviruses was termed "Leishbunyavirus" (LBV) and judged sufficiently distinct to warrant assignment within a proposed family termed "Leishbunyaviridae." Numerous relatives of trypanosomatid viruses were found in insect metatranscriptomic surveys, which likely arise from trypanosomatid microbiota. Despite extensive sampling we found no relatives of the totivirus Leishmaniavirus (LRV1/2), implying that it was acquired at about the same time the Leishmania became able to parasitize vertebrates. As viruses were found in over a quarter of isolates tested, many more are likely to be found in the >600 unsurveyed trypanosomatid species. Viral loss was occasionally observed in culture, providing potentially isogenic virus-free lines enabling studies probing the biological role of trypanosomatid viruses. These data shed important insights on the emergence of viruses within an important trypanosomatid clade relevant to human disease.

Citovat

GRYBCHUK, D., N.S. AKOPYANTS, A.Y. KOSTYGOV, A. KONOVALOVAS, L.F. LYE, D.E. DOBSON, H. ZANGGER, N. FASEL, A. BUTENKO, A.O. FROLOV, J. VOTYPKA, C.M. DAVILA-LEVY, P. KULICH, Jana MORAVCOVÁ, Pavel PLEVKA, I.B. ROGOZIN, S. SERVA, J. LUKES, S.M. BEVERLEY and V. YURCHENKO. Viral discovery and diversity in trypanosomatid protozoa with a focus on relatives of the human parasite Leishmania. Proceedings of the National Academy of Sciences of the United States of America. WASHINGTON: NATL ACAD SCIENCES, 2018, vol. 115, No 3, p. "E506"-"E515", 10 pp. ISSN 0027-8424. Available from: https://dx.doi.org/10.1073/pnas.1717806115.

@article{1505376,
   author = {Grybchuk, D. and Akopyants, N.S. and Kostygov, A.Y. and Konovalovas, A. and Lye, L.F. and Dobson, D.E. and Zangger, H. and Fasel, N. and Butenko, A. and Frolov, A.O. and Votypka, J. and dAvilaandLevy, C.M. and Kulich, P. and Moravcová, Jana and Plevka, Pavel and Rogozin, I.B. and Serva, S. and Lukes, J. and Beverley, S.M. and Yurchenko, V.},
   article_location = {WASHINGTON},
   article_number = {3},
   doi = {http://dx.doi.org/10.1073/pnas.1717806115},
   keywords = {Trypanosomatidae; Bunyavirales; persistent virus infection; coinfection; coevolution},
   language = {eng},
   issn = {0027-8424},
   journal = {Proceedings of the National Academy of Sciences of the United States of America},
   title = {Viral discovery and diversity in trypanosomatid protozoa with a focus on relatives of the human parasite Leishmania},
   volume = {115},
   year = {2018}
}

TY  - JOUR
ID  - 1505376
AU  - Grybchuk, D. - Akopyants, N.S. - Kostygov, A.Y. - Konovalovas, A. - Lye, L.F. - Dobson, D.E. - Zangger, H. - Fasel, N. - Butenko, A. - Frolov, A.O. - Votypka, J. - dAvila-Levy, C.M. - Kulich, P. - Moravcová, Jana - Plevka, Pavel - Rogozin, I.B. - Serva, S. - Lukes, J. - Beverley, S.M. - Yurchenko, V.
PY  - 2018
TI  - Viral discovery and diversity in trypanosomatid protozoa with a focus on relatives of the human parasite Leishmania
JF  - Proceedings of the National Academy of Sciences of the United States of America
VL  - 115
IS  - 3
SP  - "E506"-"E515"
EP  - "E506"-"E515"
PB  - NATL ACAD SCIENCES
SN  - 00278424
KW  - Trypanosomatidae
KW  - Bunyavirales
KW  - persistent virus infection
KW  - coinfection
KW  - coevolution
N2  - Knowledge of viral diversity is expanding greatly, but many lineages remain underexplored. We surveyed RNA viruses in 52 cultured monoxenous relatives of the human parasite Leishmania (Crithidia and Leptomonas), as well as plant-infecting Phytomonas. Leptomonas pyrrhocoris was a hotbed for viral discovery, carrying a virus (Leptomonas pyrrhocoris ostravirus 1) with a highly divergent RNA-dependent RNA polymerase missed by conventional BLAST searches, an emergent clade of tombus-like viruses, and an example of viral endogenization. A deep-branching clade of trypanosomatid narnaviruses was found, notable as Leptomonas seymouri bearing Narna-like virus 1 (LepseyNLV1) have been reported in cultures recovered from patients with visceral leishmaniasis. A deep-branching trypanosomatid viral lineage showing strong affinities to bunyaviruses was termed "Leishbunyavirus" (LBV) and judged sufficiently distinct to warrant assignment within a proposed family termed "Leishbunyaviridae." Numerous relatives of trypanosomatid viruses were found in insect metatranscriptomic surveys, which likely arise from trypanosomatid microbiota. Despite extensive sampling we found no relatives of the totivirus Leishmaniavirus (LRV1/2), implying that it was acquired at about the same time the Leishmania became able to parasitize vertebrates. As viruses were found in over a quarter of isolates tested, many more are likely to be found in the >600 unsurveyed trypanosomatid species. Viral loss was occasionally observed in culture, providing potentially isogenic virus-free lines enabling studies probing the biological role of trypanosomatid viruses. These data shed important insights on the emergence of viruses within an important trypanosomatid clade relevant to human disease.
ER  -

GRYBCHUK, D., N.S. AKOPYANTS, A.Y. KOSTYGOV, A. KONOVALOVAS, L.F. LYE, D.E. DOBSON, H. ZANGGER, N. FASEL, A. BUTENKO, A.O. FROLOV, J. VOTYPKA, C.M. DAVILA-LEVY, P. KULICH, Jana MORAVCOVÁ, Pavel PLEVKA, I.B. ROGOZIN, S. SERVA, J. LUKES, S.M. BEVERLEY and V. YURCHENKO. Viral discovery and diversity in trypanosomatid protozoa with a focus on relatives of the human parasite Leishmania. \textit{Proceedings of the National Academy of Sciences of the United States of America}. WASHINGTON: NATL ACAD SCIENCES, 2018, vol.~115, No~3, p.~''E506''-''E515'', 10 pp. ISSN~0027-8424. Available from: https://dx.doi.org/10.1073/pnas.1717806115.

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