Detailed Information on Publication Record
2018
Viral discovery and diversity in trypanosomatid protozoa with a focus on relatives of the human parasite Leishmania
GRYBCHUK, D., N.S. AKOPYANTS, A.Y. KOSTYGOV, A. KONOVALOVAS, L.F. LYE et. al.Basic information
Original name
Viral discovery and diversity in trypanosomatid protozoa with a focus on relatives of the human parasite Leishmania
Authors
GRYBCHUK, D. (203 Czech Republic), N.S. AKOPYANTS (840 United States of America), A.Y. KOSTYGOV (643 Russian Federation), A. KONOVALOVAS (440 Lithuania), L.F. LYE (840 United States of America), D.E. DOBSON (840 United States of America), H. ZANGGER (756 Switzerland), N. FASEL (756 Switzerland), A. BUTENKO (203 Czech Republic), A.O. FROLOV (643 Russian Federation), J. VOTYPKA (203 Czech Republic), C.M. DAVILA-LEVY (76 Brazil), P. KULICH (203 Czech Republic), Jana MORAVCOVÁ (203 Czech Republic, belonging to the institution), Pavel PLEVKA (203 Czech Republic, guarantor, belonging to the institution), I.B. ROGOZIN (840 United States of America), S. SERVA (440 Lithuania), J. LUKES (203 Czech Republic), S.M. BEVERLEY (840 United States of America) and V. YURCHENKO (643 Russian Federation)
Edition
Proceedings of the National Academy of Sciences of the United States of America, WASHINGTON, NATL ACAD SCIENCES, 2018, 0027-8424
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10607 Virology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 9.580
RIV identification code
RIV/00216224:14740/18:00106594
Organization unit
Central European Institute of Technology
UT WoS
000423091400021
Keywords in English
Trypanosomatidae; Bunyavirales; persistent virus infection; coinfection; coevolution
Tags
Tags
International impact, Reviewed
Změněno: 13/3/2019 11:04, Mgr. Pavla Foltynová, Ph.D.
Abstract
V originále
Knowledge of viral diversity is expanding greatly, but many lineages remain underexplored. We surveyed RNA viruses in 52 cultured monoxenous relatives of the human parasite Leishmania (Crithidia and Leptomonas), as well as plant-infecting Phytomonas. Leptomonas pyrrhocoris was a hotbed for viral discovery, carrying a virus (Leptomonas pyrrhocoris ostravirus 1) with a highly divergent RNA-dependent RNA polymerase missed by conventional BLAST searches, an emergent clade of tombus-like viruses, and an example of viral endogenization. A deep-branching clade of trypanosomatid narnaviruses was found, notable as Leptomonas seymouri bearing Narna-like virus 1 (LepseyNLV1) have been reported in cultures recovered from patients with visceral leishmaniasis. A deep-branching trypanosomatid viral lineage showing strong affinities to bunyaviruses was termed "Leishbunyavirus" (LBV) and judged sufficiently distinct to warrant assignment within a proposed family termed "Leishbunyaviridae." Numerous relatives of trypanosomatid viruses were found in insect metatranscriptomic surveys, which likely arise from trypanosomatid microbiota. Despite extensive sampling we found no relatives of the totivirus Leishmaniavirus (LRV1/2), implying that it was acquired at about the same time the Leishmania became able to parasitize vertebrates. As viruses were found in over a quarter of isolates tested, many more are likely to be found in the >600 unsurveyed trypanosomatid species. Viral loss was occasionally observed in culture, providing potentially isogenic virus-free lines enabling studies probing the biological role of trypanosomatid viruses. These data shed important insights on the emergence of viruses within an important trypanosomatid clade relevant to human disease.