Multifaceted activity of cytokinin in leaf development shapes
its size and structure in Arabidopsis

J 2019

Multifaceted activity of cytokinin in leaf development shapes its size and structure in Arabidopsis

SKALÁK, Jan, L. VERCRUYSSEN, H. CLAEYS, J. HRADILOVA, Martin ČERNÝ et. al.

Základní údaje

Originální název

Multifaceted activity of cytokinin in leaf development shapes its size and structure in Arabidopsis

Autoři

SKALÁK, Jan (203 Česká republika, garant, domácí), L. VERCRUYSSEN, H. CLAEYS, J. HRADILOVA, Martin ČERNÝ (203 Česká republika), O. NOVAK, L. PLACKOVA, I. SAIZ-FERNANDEZ, Patricie SKALÁKOVÁ (203 Česká republika), F. COPPENS, S. DHONDT, S. KOUKALOVA, J. ZOUHAR, D. INZE a Břetislav BRZOBOHATÝ (203 Česká republika)

Vydání

Plant Journal, Oxford, Blackwell Publishing Ltd, 2019, 0960-7412

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.141

Kód RIV

RIV/00216224:14740/19:00109276

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1111/tpj.14285

UT WoS

000462684900002

Klíčová slova anglicky

Arabidopsis thaliana; cytokinin; leaf development; proteome; transcriptome

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 3. 2020 19:28, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

The phytohormone cytokinin has been shown to affect many aspects of plant development ranging from the regulation of the shoot apical meristem to leaf senescence. However, some studies have reported contradictory effects of cytokinin on leaf physiology. Therefore cytokinin treatments cause both chlorosis and increased greening and both lead to decrease or increase in cell size. To elucidate this multifaceted role of cytokinin in leaf development, we have employed a system of temporal controls over the cytokinin pool and investigated the consequences of modulated cytokinin levels in the third leaf of Arabidopsis. We show that, at the cell proliferation phase, cytokinin is needed to maintain cell proliferation by blocking the transition to cell expansion and the onset of photosynthesis. Transcriptome profiling revealed regulation by cytokinin of a gene suite previously shown to affect cell proliferation and expansion and thereby a molecular mechanism by which cytokinin modulates a molecular network underlying the cellular responses. During the cell expansion phase, cytokinin stimulates cell expansion and differentiation. Consequently, a cytokinin excess at the cell expansion phase results in an increased leaf and rosette size fueled by higher cell expansion rate, yielding higher shoot biomass. Proteome profiling revealed the stimulation of primary metabolism by cytokinin, in line with an increased sugar content that is expected to increase turgor pressure, representing the driving force of cell expansion. Therefore, the developmental timing of cytokinin content fluctuations, together with a tight control of primary metabolism, is a key factor mediating transitions from cell proliferation to cell expansion in leaves.

Návaznosti

EF16_019/0000738, projekt VaV

Název: Centrum experimentální biologie rostlin

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

SKALÁK, Jan, L. VERCRUYSSEN, H. CLAEYS, J. HRADILOVA, Martin ČERNÝ, O. NOVAK, L. PLACKOVA, I. SAIZ-FERNANDEZ, Patricie SKALÁKOVÁ, F. COPPENS, S. DHONDT, S. KOUKALOVA, J. ZOUHAR, D. INZE a Břetislav BRZOBOHATÝ. Multifaceted activity of cytokinin in leaf development shapes its size and structure in Arabidopsis. Plant Journal. Oxford: Blackwell Publishing Ltd, 2019, roč. 97, č. 5, s. 805-824. ISSN 0960-7412. Dostupné z: https://dx.doi.org/10.1111/tpj.14285.

@article{1505679,
   author = {Skalák, Jan and Vercruyssen, L. and Claeys, H. and Hradilova, J. and Černý, Martin and Novak, O. and Plackova, L. and SaizandFernandez, I. and Skaláková, Patricie and Coppens, F. and Dhondt, S. and Koukalova, S. and Zouhar, J. and Inze, D. and Brzobohatý, Břetislav},
   article_location = {Oxford},
   article_number = {5},
   doi = {http://dx.doi.org/10.1111/tpj.14285},
   keywords = {Arabidopsis thaliana; cytokinin; leaf development; proteome; transcriptome},
   language = {eng},
   issn = {0960-7412},
   journal = {Plant Journal},
   title = {Multifaceted activity of cytokinin in leaf development shapes its size and structure in Arabidopsis},
   url = {https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.14285},
   volume = {97},
   year = {2019}
}

TY  - JOUR
ID  - 1505679
AU  - Skalák, Jan - Vercruyssen, L. - Claeys, H. - Hradilova, J. - Černý, Martin - Novak, O. - Plackova, L. - Saiz-Fernandez, I. - Skaláková, Patricie - Coppens, F. - Dhondt, S. - Koukalova, S. - Zouhar, J. - Inze, D. - Brzobohatý, Břetislav
PY  - 2019
TI  - Multifaceted activity of cytokinin in leaf development shapes its size and structure in Arabidopsis
JF  - Plant Journal
VL  - 97
IS  - 5
SP  - 805-824
EP  - 805-824
PB  - Blackwell Publishing Ltd
SN  - 09607412
KW  - Arabidopsis thaliana
KW  - cytokinin
KW  - leaf development
KW  - proteome
KW  - transcriptome
UR  - https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.14285
L2  - https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.14285
N2  - The phytohormone cytokinin has been shown to affect many aspects of plant development ranging from the regulation of the shoot apical meristem to leaf senescence. However, some studies have reported contradictory effects of cytokinin on leaf physiology. Therefore cytokinin treatments cause both chlorosis and increased greening and both lead to decrease or increase in cell size. To elucidate this multifaceted role of cytokinin in leaf development, we have employed a system of temporal controls over the cytokinin pool and investigated the consequences of modulated cytokinin levels in the third leaf of Arabidopsis. We show that, at the cell proliferation phase, cytokinin is needed to maintain cell proliferation by blocking the transition to cell expansion and the onset of photosynthesis. Transcriptome profiling revealed regulation by cytokinin of a gene suite previously shown to affect cell proliferation and expansion and thereby a molecular mechanism by which cytokinin modulates a molecular network underlying the cellular responses. During the cell expansion phase, cytokinin stimulates cell expansion and differentiation. Consequently, a cytokinin excess at the cell expansion phase results in an increased leaf and rosette size fueled by higher cell expansion rate, yielding higher shoot biomass. Proteome profiling revealed the stimulation of primary metabolism by cytokinin, in line with an increased sugar content that is expected to increase turgor pressure, representing the driving force of cell expansion. Therefore, the developmental timing of cytokinin content fluctuations, together with a tight control of primary metabolism, is a key factor mediating transitions from cell proliferation to cell expansion in leaves.
ER  -

SKALÁK, Jan, L. VERCRUYSSEN, H. CLAEYS, J. HRADILOVA, Martin ČERNÝ, O. NOVAK, L. PLACKOVA, I. SAIZ-FERNANDEZ, Patricie SKALÁKOVÁ, F. COPPENS, S. DHONDT, S. KOUKALOVA, J. ZOUHAR, D. INZE a Břetislav BRZOBOHATÝ. Multifaceted activity of cytokinin in leaf development shapes its size and structure in Arabidopsis. \textit{Plant Journal}. Oxford: Blackwell Publishing Ltd, 2019, roč.~97, č.~5, s.~805-824. ISSN~0960-7412. Dostupné z: https://dx.doi.org/10.1111/tpj.14285.

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