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@article{1505749,
author = {Füzik, Tibor and Formanova, P. and Ruzek, D. and Yoshii, K. and Niedrig, M. and Plevka, Pavel},
article_location = {London},
article_number = {JAN},
doi = {http://dx.doi.org/10.1038/s41467-018-02882-0},
keywords = {WEST-NILE-VIRUS; DENGUE VIRUS; MEMBRANE-FUSION; ENVELOPE GLYCOPROTEIN; ELECTRON-MICROSCOPY; ZIKA VIRUS; CELL ENTRY; ACIDIC PH; MATURATION; PROTEINS},
language = {eng},
issn = {2041-1723},
journal = {Nature Communications},
title = {Structure of tick-borne encephalitis virus and its neutralization by a monoclonal antibody},
volume = {9},
year = {2018}
}
TY - JOUR
ID - 1505749
AU - Füzik, Tibor - Formanova, P. - Ruzek, D. - Yoshii, K. - Niedrig, M. - Plevka, Pavel
PY - 2018
TI - Structure of tick-borne encephalitis virus and its neutralization by a monoclonal antibody
JF - Nature Communications
VL - 9
IS - JAN
SP - 436
EP - 436
PB - Nature Publishing Group
SN - 20411723
KW - WEST-NILE-VIRUS
KW - DENGUE VIRUS
KW - MEMBRANE-FUSION
KW - ENVELOPE GLYCOPROTEIN
KW - ELECTRON-MICROSCOPY
KW - ZIKA VIRUS
KW - CELL ENTRY
KW - ACIDIC PH
KW - MATURATION
KW - PROTEINS
N2 - Tick-borne encephalitis virus (TBEV) causes 13,000 cases of human meningitis and encephalitis annually. However, the structure of the TBEV virion and its interactions with antibodies are unknown. Here, we present cryo-EM structures of the native TBEV virion and its complex with Fab fragments of neutralizing antibody 19/1786. Flavivirus genome delivery depends on membrane fusion that is triggered at low pH. The virion structure indicates that the repulsive interactions of histidine side chains, which become protonated at low pH, may contribute to the disruption of heterotetramers of the TBEV envelope and membrane proteins and induce detachment of the envelope protein ectodomains from the virus membrane. The Fab fragments bind to 120 out of the 180 envelope glycoproteins of the TBEV virion. Unlike most of the previously studied flavivirus-neutralizing antibodies, the Fab fragments do not lock the E-proteins in the native-like arrangement, but interfere with the process of virus-induced membrane fusion.
ER -
FÜZIK, Tibor, P. FORMANOVA, D. RUZEK, K. YOSHII, M. NIEDRIG a Pavel PLEVKA. Structure of tick-borne encephalitis virus and its neutralization by a monoclonal antibody. \textit{Nature Communications}. London: Nature Publishing Group, 2018, roč.~9, JAN, s.~436-446. ISSN~2041-1723. Dostupné z: https://dx.doi.org/10.1038/s41467-018-02882-0.
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