CLARK, S., J.B. MYERS, A. KING, Radovan FIALA, Jiří NOVÁČEK, G. PEARCE, J. HEIERHORST, S.L. REICHOW a E.J. BARBAR. Multivalency regulates activity in an intrinsically disordered transcription factor. elife. CAMBRIDGE: ELIFE SCIENCES PUBLICATIONS LTD, 2018, roč. 7, MAY, s. 1-28. ISSN 2050-084X. Dostupné z: https://dx.doi.org/10.7554/eLife.36258. |
Další formáty:
BibTeX
LaTeX
RIS
@article{1507897, author = {Clark, S. and Myers, J.B. and King, A. and Fiala, Radovan and Nováček, Jiří and Pearce, G. and Heierhorst, J. and Reichow, S.L. and Barbar, E.J.}, article_location = {CAMBRIDGE}, article_number = {MAY}, doi = {http://dx.doi.org/10.7554/eLife.36258}, keywords = {DYNEIN LIGHT-CHAIN; BINDING PROTEIN CBP; C-TERMINAL DOMAIN; MULTISITE PHOSPHORYLATION; UNSTRUCTURED REGION; DNA-BINDING; LC8; COMPLEX; DYNLL1; ASCIZ}, language = {eng}, issn = {2050-084X}, journal = {elife}, title = {Multivalency regulates activity in an intrinsically disordered transcription factor}, volume = {7}, year = {2018} }
TY - JOUR ID - 1507897 AU - Clark, S. - Myers, J.B. - King, A. - Fiala, Radovan - Nováček, Jiří - Pearce, G. - Heierhorst, J. - Reichow, S.L. - Barbar, E.J. PY - 2018 TI - Multivalency regulates activity in an intrinsically disordered transcription factor JF - elife VL - 7 IS - MAY SP - 1-28 EP - 1-28 PB - ELIFE SCIENCES PUBLICATIONS LTD SN - 2050084X KW - DYNEIN LIGHT-CHAIN KW - BINDING PROTEIN CBP KW - C-TERMINAL DOMAIN KW - MULTISITE PHOSPHORYLATION KW - UNSTRUCTURED REGION KW - DNA-BINDING KW - LC8 KW - COMPLEX KW - DYNLL1 KW - ASCIZ N2 - The transcription factor ASCIZ (ATMIN, ZNF822) has an unusually high number of recognition motifs for the product of its main target gene, the hub protein LC8 (DYNLL1). Using a combination of biophysical methods, structural analysis by NMR and electron microscopy, and cellular transcription assays, we developed a model that proposes a concerted role of intrinsic disorder and multiple LC8 binding events in regulating LC8 transcription. We demonstrate that the long intrinsically disordered C -terminal domain of ASCIZ binds LC8 to form a dynamic ensemble of complexes with a gradient of transcriptional activity that is inversely proportional to LC8 occupancy. The preference for low occupancy complexes at saturating LC8 concentrations with both human and Drosophila ASCIZ indicates that negative cooperativity is an important feature of ASCIZ-LC8 interactions. The prevalence of intrinsic disorder and multivalency among transcription factors suggests that formation of heterogeneous, dynamic complexes is a widespread mechanism for tuning transcriptional regulation. ER -
CLARK, S., J.B. MYERS, A. KING, Radovan FIALA, Jiří NOVÁČEK, G. PEARCE, J. HEIERHORST, S.L. REICHOW a E.J. BARBAR. Multivalency regulates activity in an intrinsically disordered transcription factor. \textit{elife}. CAMBRIDGE: ELIFE SCIENCES PUBLICATIONS LTD, 2018, roč.~7, MAY, s.~1-28. ISSN~2050-084X. Dostupné z: https://dx.doi.org/10.7554/eLife.36258.
|