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@article{1508097,
author = {Rössner, Pavel and Vrbová, Kristýna and Strapáčová, Simona and Rossnerová, Andrea and Ambroz, Antonin and Brzicova, Tana and Libalová, Helena and Javorková, Eliška and Kulich, Pavel and Večeřa, Zbyněk and Mikuška, Pavel and Coufalík, Pavel and Krumal, Kamil and Čapka, Lukáš and Dočekal, Bohumil and Moravec, Pavel and Šerý, Omar and Míšek, Ivan and Fictum, Petr and Fišer, Karel and Machala, Miroslav and Topinka, Jan},
article_number = {1},
doi = {http://dx.doi.org/10.1093/toxsci/kfy288},
keywords = {nanoparticles; mice; inhalation},
language = {eng},
issn = {1096-6080},
journal = {Toxicological sciences},
note = {Žádný z autorů nemá afiliaci k MU, nelze tedy výsledek přenášet do RIV.},
title = {Inhalation of ZnO Nanoparticles: Splice Junction Expression and Alternative Splicing in Mice},
url = {http://dx.doi.org/10.1093/toxsci/kfy288},
volume = {168},
year = {2019}
}
TY - JOUR
ID - 1508097
AU - Rössner, Pavel - Vrbová, Kristýna - Strapáčová, Simona - Rossnerová, Andrea - Ambroz, Antonin - Brzicova, Tana - Libalová, Helena - Javorková, Eliška - Kulich, Pavel - Večeřa, Zbyněk - Mikuška, Pavel - Coufalík, Pavel - Krumal, Kamil - Čapka, Lukáš - Dočekal, Bohumil - Moravec, Pavel - Šerý, Omar - Míšek, Ivan - Fictum, Petr - Fišer, Karel - Machala, Miroslav - Topinka, Jan
PY - 2019
TI - Inhalation of ZnO Nanoparticles: Splice Junction Expression and Alternative Splicing in Mice
JF - Toxicological sciences
VL - 168
IS - 1
SP - 190-200
EP - 190-200
PB - Academic Press
SN - 10966080
N1 - Žádný z autorů nemá afiliaci k MU, nelze tedy výsledek přenášet do RIV.
KW - nanoparticles
KW - mice
KW - inhalation
UR - http://dx.doi.org/10.1093/toxsci/kfy288
L2 - http://dx.doi.org/10.1093/toxsci/kfy288
N2 - Despite the wide application of nanomaterials, toxicity studies of nanoparticles (NP) are often limited to in vitro cell models, and the biological impact of NP exposure in mammals has not been thoroughly investigated. Zinc oxide (ZnO) NPs are commonly used in various consumer products. To evaluate the effects of the inhalation of ZnO NP in mice, we studied splice junction expression in the lungs as a proxy to gene expression changes analysis. Female ICR mice were treated with 6.46 * 104 and 1.93 * 106 NP/cm3 for 3 days and 3 months, respectively. An analysis of differential expression and alternative splicing events in 298 targets (splice junctions) of 68 genes involved in the processes relevant to the biological effects of ZnO NP was conducted using next-generation sequencing. Three days of exposure resulted in the upregulation of IL-6 and downregulation of BID, GSR, NF-kB2, PTGS2, SLC11A2, and TXNRD1 splice junction expression; 3 months of exposure increased the expression of splice junctions in ALDH3A1, APAF1, BID, CASP3, DHCR7, GCLC, GCLM, GSR, GSS, EHHADH, FAS, HMOX-1, IFNgamma, NF-kB1, NQO-1, PTGS1, PTGS2, RAD51, RIPK2, SRXN1, TRAF6, and TXNRD1. Alternative splicing of TRAF6 and TXNRD1 was induced after 3 days of exposure to 1.93 * 106 NP/cm3. In summary, we observed changes of splice junction expression in genes involved in oxidative stress, apoptosis, immune response, inflammation, and DNA repair, as well as the induction of alternative splicing in genes associated with oxidative stress and inflammation. Our data indicate the potential negative biological effects of ZnO NP inhalation.
ER -
RÖSSNER, Pavel, Kristýna VRBOVÁ, Simona STRAPÁČOVÁ, Andrea ROSSNEROVÁ, Antonin AMBROZ, Tana BRZICOVA, Helena LIBALOVÁ, Eliška JAVORKOVÁ, Pavel KULICH, Zbyněk VEČEŘA, Pavel MIKUŠKA, Pavel COUFALÍK, Kamil KRUMAL, Lukáš ČAPKA, Bohumil DOČEKAL, Pavel MORAVEC, Omar ŠERÝ, Ivan MÍŠEK, Petr FICTUM, Karel FIŠER, Miroslav MACHALA a Jan TOPINKA. Inhalation of ZnO Nanoparticles: Splice Junction Expression and Alternative Splicing in Mice. \textit{Toxicological sciences}. Academic Press, 2019, roč.~168, č.~1, s.~190-200. ISSN~1096-6080. Dostupné z: https://dx.doi.org/10.1093/toxsci/kfy288.
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