2019
Inhalation of ZnO Nanoparticles: Splice Junction Expression and Alternative Splicing in Mice
RÖSSNER, Pavel, Kristýna VRBOVÁ, Simona STRAPÁČOVÁ, Andrea ROSSNEROVÁ, Antonin AMBROZ et. al.Základní údaje
Originální název
Inhalation of ZnO Nanoparticles: Splice Junction Expression and Alternative Splicing in Mice
Autoři
RÖSSNER, Pavel, Kristýna VRBOVÁ, Simona STRAPÁČOVÁ, Andrea ROSSNEROVÁ, Antonin AMBROZ, Tana BRZICOVA, Helena LIBALOVÁ, Eliška JAVORKOVÁ, Pavel KULICH, Zbyněk VEČEŘA, Pavel MIKUŠKA, Pavel COUFALÍK, Kamil KRUMAL, Lukáš ČAPKA, Bohumil DOČEKAL, Pavel MORAVEC, Omar ŠERÝ, Ivan MÍŠEK, Petr FICTUM, Karel FIŠER, Miroslav MACHALA a Jan TOPINKA
Vydání
Toxicological sciences, Academic Press, 2019, 1096-6080
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30108 Toxicology
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 3.703
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000462865100017
Klíčová slova česky
nanočástice; myš; inhalace
Klíčová slova anglicky
nanoparticles; mice; inhalation
Štítky
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 12. 5. 2021 12:48, Mgr. Marie Šípková, DiS.
Anotace
V originále
Despite the wide application of nanomaterials, toxicity studies of nanoparticles (NP) are often limited to in vitro cell models, and the biological impact of NP exposure in mammals has not been thoroughly investigated. Zinc oxide (ZnO) NPs are commonly used in various consumer products. To evaluate the effects of the inhalation of ZnO NP in mice, we studied splice junction expression in the lungs as a proxy to gene expression changes analysis. Female ICR mice were treated with 6.46 * 104 and 1.93 * 106 NP/cm3 for 3 days and 3 months, respectively. An analysis of differential expression and alternative splicing events in 298 targets (splice junctions) of 68 genes involved in the processes relevant to the biological effects of ZnO NP was conducted using next-generation sequencing. Three days of exposure resulted in the upregulation of IL-6 and downregulation of BID, GSR, NF-kB2, PTGS2, SLC11A2, and TXNRD1 splice junction expression; 3 months of exposure increased the expression of splice junctions in ALDH3A1, APAF1, BID, CASP3, DHCR7, GCLC, GCLM, GSR, GSS, EHHADH, FAS, HMOX-1, IFNgamma, NF-kB1, NQO-1, PTGS1, PTGS2, RAD51, RIPK2, SRXN1, TRAF6, and TXNRD1. Alternative splicing of TRAF6 and TXNRD1 was induced after 3 days of exposure to 1.93 * 106 NP/cm3. In summary, we observed changes of splice junction expression in genes involved in oxidative stress, apoptosis, immune response, inflammation, and DNA repair, as well as the induction of alternative splicing in genes associated with oxidative stress and inflammation. Our data indicate the potential negative biological effects of ZnO NP inhalation.