SUPPRESION OF PROTEIN SAMPLE LOSSES IN AUTOSAMPLER VIALS

J 2013

SUPPRESION OF PROTEIN SAMPLE LOSSES IN AUTOSAMPLER VIALS

STEJSKAL, Karel, David POTĚŠIL a Zbyněk ZDRÁHAL

Základní údaje

Originální název

SUPPRESION OF PROTEIN SAMPLE LOSSES IN AUTOSAMPLER VIALS

Autoři

STEJSKAL, Karel (203 Česká republika, domácí), David POTĚŠIL (203 Česká republika, domácí) a Zbyněk ZDRÁHAL (203 Česká republika, garant, domácí)

Vydání

CHEMICKÉ LISTY, Praha, Česká společnost chemická, 2013, 0009-2770

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.196

Kód RIV

RIV/00216224:14740/13:00107015

Organizační jednotka

Středoevropský technologický institut

UT WoS

000328730800017

Klíčová slova anglicky

RECOVERY; PEPTIDES

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 30. 4. 2020 10:12, Mgr. Michal Petr

Anotace

V originále

In the present study, we assessed peptide losses during sample storage in autosampler vials. We evaluated the possibility to suppress the adsorption effects by changing the composition of the sample solution injected into the liquid chromatography coupled with tandem mass spectrometry system (LC-MS/MS) and by use of different material of autosampler vials. As a model sample, a tryptic digest of six bovine proteins (B6E) in the amount of 1 fmol per protein was used for comparative experiments. The combination of a polypropylene vial and solution of poly(ethylene glycol) (PEG) (0.001%) or a mixture of high concentrated urea and thiourea (5M and 1M) as injection solutions provided the best results in terms of number of significantly identified peptides (P < 0.05). These conclusions were confirmed by analyses of a real sample with intermediate complexity. Addition of PEG into the real sample solution proved to prevent higher losses, concerning mainly hydrophobic peptides, during up to 48 h storage in the autosampler in comparison with a formic acid solution and even with a solution of highly concentrated urea and thiourea(1).

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

GBP206/12/G151, projekt VaV

Název: Centrum nových přístupů k bioanalýze a molekulární diagnostice

Citovat

STEJSKAL, Karel, David POTĚŠIL a Zbyněk ZDRÁHAL. SUPPRESION OF PROTEIN SAMPLE LOSSES IN AUTOSAMPLER VIALS. CHEMICKÉ LISTY. Praha: Česká společnost chemická, 2013, roč. 107, č. 2013, s. "S324"-"S326", 3 s. ISSN 0009-2770.

@article{1512558,
   author = {Stejskal, Karel and Potěšil, David and Zdráhal, Zbyněk},
   article_location = {Praha},
   article_number = {2013},
   keywords = {RECOVERY; PEPTIDES},
   language = {eng},
   issn = {0009-2770},
   journal = {CHEMICKÉ LISTY},
   title = {SUPPRESION OF PROTEIN SAMPLE LOSSES IN AUTOSAMPLER VIALS},
   volume = {107},
   year = {2013}
}

TY  - JOUR
ID  - 1512558
AU  - Stejskal, Karel - Potěšil, David - Zdráhal, Zbyněk
PY  - 2013
TI  - SUPPRESION OF PROTEIN SAMPLE LOSSES IN AUTOSAMPLER VIALS
JF  - CHEMICKÉ LISTY
VL  - 107
IS  - 2013
SP  - "S324"-"S326"
EP  - "S324"-"S326"
PB  - Česká společnost chemická
SN  - 00092770
KW  - RECOVERY
KW  - PEPTIDES
N2  - In the present study, we assessed peptide losses during sample storage in autosampler vials. We evaluated the possibility to suppress the adsorption effects by changing the composition of the sample solution injected into the liquid chromatography coupled with tandem mass spectrometry system (LC-MS/MS) and by use of different material of autosampler vials. As a model sample, a tryptic digest of six bovine proteins (B6E) in the amount of 1 fmol per protein was used for comparative experiments. The combination of a polypropylene vial and solution of poly(ethylene glycol) (PEG) (0.001%) or a mixture of high concentrated urea and thiourea (5M and 1M) as injection solutions provided the best results in terms of number of significantly identified peptides (P < 0.05). These conclusions were confirmed by analyses of a real sample with intermediate complexity. Addition of PEG into the real sample solution proved to prevent higher losses, concerning mainly hydrophobic peptides, during up to 48 h storage in the autosampler in comparison with a formic acid solution and even with a solution of highly concentrated urea and thiourea(1).
ER  -

STEJSKAL, Karel, David POTĚŠIL a Zbyněk ZDRÁHAL. SUPPRESION OF PROTEIN SAMPLE LOSSES IN AUTOSAMPLER VIALS. \textit{CHEMICKÉ LISTY}. Praha: Česká společnost chemická, 2013, roč.~107, č.~2013, s.~''S324''-''S326'', 3 s. ISSN~0009-2770.

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