Variability of resistance plasmids in coagulase-negative
staphylococci and their importance as a reservoir of
antimicrobial resistance

J 2019

Variability of resistance plasmids in coagulase-negative staphylococci and their importance as a reservoir of antimicrobial resistance

FIŠAROVÁ, Lenka, Roman PANTŮČEK, Tibor BOTKA a Jiří DOŠKAŘ

Základní údaje

Originální název

Variability of resistance plasmids in coagulase-negative staphylococci and their importance as a reservoir of antimicrobial resistance

Autoři

FIŠAROVÁ, Lenka (203 Česká republika, domácí), Roman PANTŮČEK (203 Česká republika, domácí), Tibor BOTKA (203 Česká republika, domácí) a Jiří DOŠKAŘ (203 Česká republika, garant, domácí)

Vydání

Research in Microbiology, Amsterdam, Elsevier Science, 2019, 0923-2508

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 3.217

Kód RIV

RIV/00216224:14310/19:00108326

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.resmic.2018.11.004

UT WoS

000461900000006

Klíčová slova anglicky

Coagulase-negative staphylococci; Resistance plasmids; Antibiotics; Interspecies horizontal gene transfer; Electroporation

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 3. 2020 13:27, Mgr. Marie Šípková, DiS.

Anotace

V originále

Coagulase-negative staphylococci (CoNS) are an important cause of human and animal diseases. Treatment of these diseases is complicated by their common antimicrobial resistance, caused by overuse of antibiotics in hospital and veterinary environment. Therefore, they are assumed to serve as a reservoir of resistance genes often located on plasmids. In this study, we analyzed plasmid content in 62 strains belonging to 10 CoNS species of human and veterinary origin. In 48 (77%) strains analyzed, 107 different plasmids were detected, and only some of them showed similarities with plasmids found previously. In total, seven different antimicrobial-resistance genes carried by plasmids were identified. Five of the CoNS staphylococci carried plasmids identical with either those of other CoNS species tested, or a well characterized Staphylococcus aureus strain COL, suggesting plasmid dissemination through horizontal transfer. To demonstrate the possibility of horizontal transfer, we performed electroporation of four resistance plasmids among Staphylococcus epidermidis, Staphylococcus petrasii, and coagulase-positive S. aureus strains. Plasmids were transferred unchanged, were stably maintained in recipient strains, and expressed resistance genes. Our work demonstrates a great variability of plasmids in human and veterinary staphylococcal strains and their ability to maintain and express resistance plasmids from other staphylococcal species.

Návaznosti

MUNI/A/0824/2017, interní kód MU

Název: Podpora výzkumné činnosti studentů molekulární biologie a genetiky 6 (Akronym: MolBiolGen)

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů molekulární biologie a genetiky 6, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

QJ1510216, projekt VaV

Název: Fágová terapie infekcí vyvolaných Staphylococcus aureus v chovech hospodářských zvířat (Akronym: StafyFag)

Investor: Ministerstvo zemědělství ČR, Fágová terapie infekcí vyvolaných Staphylococcus aureus v chovech hospodářských zvířat

Přiložené soubory

1-s2.0-S0923250818301608-main.pdf

Požádat o autorskou verzi souboru

Citovat

FIŠAROVÁ, Lenka, Roman PANTŮČEK, Tibor BOTKA a Jiří DOŠKAŘ. Variability of resistance plasmids in coagulase-negative staphylococci and their importance as a reservoir of antimicrobial resistance. Research in Microbiology. Amsterdam: Elsevier Science, 2019, roč. 170, č. 2, s. 105-111. ISSN 0923-2508. Dostupné z: https://dx.doi.org/10.1016/j.resmic.2018.11.004.

@article{1514537,
   author = {Fišarová, Lenka and Pantůček, Roman and Botka, Tibor and Doškař, Jiří},
   article_location = {Amsterdam},
   article_number = {2},
   doi = {http://dx.doi.org/10.1016/j.resmic.2018.11.004},
   keywords = {Coagulase-negative staphylococci; Resistance plasmids; Antibiotics; Interspecies horizontal gene transfer; Electroporation},
   language = {eng},
   issn = {0923-2508},
   journal = {Research in Microbiology},
   title = {Variability of resistance plasmids in coagulase-negative staphylococci and their importance as a reservoir of antimicrobial resistance},
   url = {https://www.sciencedirect.com/science/article/pii/S0923250818301608},
   volume = {170},
   year = {2019}
}

TY  - JOUR
ID  - 1514537
AU  - Fišarová, Lenka - Pantůček, Roman - Botka, Tibor - Doškař, Jiří
PY  - 2019
TI  - Variability of resistance plasmids in coagulase-negative staphylococci and their importance as a reservoir of antimicrobial resistance
JF  - Research in Microbiology
VL  - 170
IS  - 2
SP  - 105-111
EP  - 105-111
PB  - Elsevier Science
SN  - 09232508
KW  - Coagulase-negative staphylococci
KW  - Resistance plasmids
KW  - Antibiotics
KW  - Interspecies horizontal gene transfer
KW  - Electroporation
UR  - https://www.sciencedirect.com/science/article/pii/S0923250818301608
L2  - https://www.sciencedirect.com/science/article/pii/S0923250818301608
N2  - Coagulase-negative staphylococci (CoNS) are an important cause of human and animal diseases. Treatment of these diseases is complicated by their common antimicrobial resistance, caused by overuse of antibiotics in hospital and veterinary environment. Therefore, they are assumed to serve as a reservoir of resistance genes often located on plasmids. In this study, we analyzed plasmid content in 62 strains belonging to 10 CoNS species of human and veterinary origin. In 48 (77%) strains analyzed, 107 different plasmids were detected, and only some of them showed similarities with plasmids found previously. In total, seven different antimicrobial-resistance genes carried by plasmids were identified. Five of the CoNS staphylococci carried plasmids identical with either those of other CoNS species tested, or a well characterized Staphylococcus aureus strain COL, suggesting plasmid dissemination through horizontal transfer. To demonstrate the possibility of horizontal transfer, we performed electroporation of four resistance plasmids among Staphylococcus epidermidis, Staphylococcus petrasii, and coagulase-positive S. aureus strains. Plasmids were transferred unchanged, were stably maintained in recipient strains, and expressed resistance genes. Our work demonstrates a great variability of plasmids in human and veterinary staphylococcal strains and their ability to maintain and express resistance plasmids from other staphylococcal species.
ER  -

FIŠAROVÁ, Lenka, Roman PANTŮČEK, Tibor BOTKA a Jiří DOŠKAŘ. Variability of resistance plasmids in coagulase-negative staphylococci and their importance as a reservoir of antimicrobial resistance. \textit{Research in Microbiology}. Amsterdam: Elsevier Science, 2019, roč.~170, č.~2, s.~105-111. ISSN~0923-2508. Dostupné z: https://dx.doi.org/10.1016/j.resmic.2018.11.004.

Podrobný výpis o publikaci