DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Ivana HRADILOVÁ SVÍŽENSKÁ, Václav BRÁZDA, Marcela KOHOUTKOVÁ a Marek JOUKAL. A Conditioning Sciatic Nerve Lesion Triggers a Pro-regenerative State in Primary Sensory Neurons Also of Dorsal Root Ganglia Non-associated With the Damaged Nerve. Frontiers in Cellular Neuroscience. Lausanne: Frontiers, 2019, roč. 13, č. 11, s. 1-16. ISSN 1662-5102. Dostupné z: https://dx.doi.org/10.3389/fncel.2019.00011. |
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@article{1521116, author = {Dubový, Petr and Klusáková, Ilona and Hradilová Svíženská, Ivana and Brázda, Václav and Kohoutková, Marcela and Joukal, Marek}, article_location = {Lausanne}, article_number = {11}, doi = {http://dx.doi.org/10.3389/fncel.2019.00011}, keywords = {unilateral nerve injury; primary sensory neurons; pro-regenerative state; GAP-43; SCG-10; IL-6; ulnar nerve crush; neurite outgrowth assay}, language = {eng}, issn = {1662-5102}, journal = {Frontiers in Cellular Neuroscience}, title = {A Conditioning Sciatic Nerve Lesion Triggers a Pro-regenerative State in Primary Sensory Neurons Also of Dorsal Root Ganglia Non-associated With the Damaged Nerve}, url = {http://dx.doi.org/10.3389/fncel.2019.00011}, volume = {13}, year = {2019} }
TY - JOUR ID - 1521116 AU - Dubový, Petr - Klusáková, Ilona - Hradilová Svíženská, Ivana - Brázda, Václav - Kohoutková, Marcela - Joukal, Marek PY - 2019 TI - A Conditioning Sciatic Nerve Lesion Triggers a Pro-regenerative State in Primary Sensory Neurons Also of Dorsal Root Ganglia Non-associated With the Damaged Nerve JF - Frontiers in Cellular Neuroscience VL - 13 IS - 11 SP - 1-16 EP - 1-16 PB - Frontiers SN - 16625102 KW - unilateral nerve injury KW - primary sensory neurons KW - pro-regenerative state KW - GAP-43 KW - SCG-10 KW - IL-6 KW - ulnar nerve crush KW - neurite outgrowth assay UR - http://dx.doi.org/10.3389/fncel.2019.00011 L2 - http://dx.doi.org/10.3389/fncel.2019.00011 N2 - The primary sensory neurons of dorsal root ganglia (DRG) are a very useful model to study the neuronal regenerative program that is a prerequisite for successful axon regeneration after peripheral nerve injury. Seven days after a unilateral sciatic nerve injury by compression or transection, we detected a bilateral increase in growth-associated protein-43 (GAP-43) and superior cervical ganglion-10 (SCG-10) mRNA and protein levels not only in DRG neurons of lumbar spinal cord segments (L4-L5) associated with injured nerve, but also in remote cervical segments (C6-C8). The increase in regeneration-associated proteins in the cervical DRG neurons was associated with the greater length of regenerated axons 1 day after ulnar nerve crush following prior sciatic nerve injury as compared to controls with only ulnar nerve crush. The increased axonal regeneration capacity of cervical DRG neurons after a prior conditioning sciatic nerve lesion was confirmed by neurite outgrowth assay of in vitro cultivated DRG neurons. Intrathecal injection of IL-6 or a JAK2 inhibitor (AG490) revealed a role for the IL-6 signaling pathway in activating the pro-regenerative state in remote DRG neurons. Our results suggest that the pro-regenerative state induced in the DRG neurons non-associated with the injured nerve reflects a systemic reaction of these neurons to unilateral sciatic nerve injury. ER -
DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Ivana HRADILOVÁ SVÍŽENSKÁ, Václav BRÁZDA, Marcela KOHOUTKOVÁ a Marek JOUKAL. A Conditioning Sciatic Nerve Lesion Triggers a Pro-regenerative State in Primary Sensory Neurons Also of Dorsal Root Ganglia Non-associated With the Damaged Nerve. \textit{Frontiers in Cellular Neuroscience}. Lausanne: Frontiers, 2019, roč.~13, č.~11, s.~1-16. ISSN~1662-5102. Dostupné z: https://dx.doi.org/10.3389/fncel.2019.00011.
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