BOTKA, Tibor, Roman PANTŮČEK, Ivana MAŠLAŇOVÁ, Martin BENEŠÍK, Petr PETRÁŠ, Vladislava RŮŽIČKOVÁ, Pavla HAVLÍČKOVÁ, Marian VARGA, Helena ŽEMLIČKOVÁ, Ivana KOLÁČKOVÁ, Martina FLORIANOVÁ, Vladislav JAKUBŮ, Renata KARPÍŠKOVÁ and Jiří DOŠKAŘ. Lytic and genomic properties of spontaneous host-range Kayvirus mutants prove their suitability for upgrading phage therapeutics against staphylococci. Scientific Reports. London: Nature Publishing Group, 2019, vol. 9, No 1, p. 5475-5486. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-019-41868-w.
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Basic information
Original name Lytic and genomic properties of spontaneous host-range Kayvirus mutants prove their suitability for upgrading phage therapeutics against staphylococci
Authors BOTKA, Tibor (203 Czech Republic, belonging to the institution), Roman PANTŮČEK (203 Czech Republic, guarantor, belonging to the institution), Ivana MAŠLAŇOVÁ (203 Czech Republic, belonging to the institution), Martin BENEŠÍK (203 Czech Republic, belonging to the institution), Petr PETRÁŠ (203 Czech Republic), Vladislava RŮŽIČKOVÁ (203 Czech Republic, belonging to the institution), Pavla HAVLÍČKOVÁ (203 Czech Republic, belonging to the institution), Marian VARGA (203 Czech Republic, belonging to the institution), Helena ŽEMLIČKOVÁ (203 Czech Republic), Ivana KOLÁČKOVÁ (203 Czech Republic), Martina FLORIANOVÁ (203 Czech Republic), Vladislav JAKUBŮ (203 Czech Republic), Renata KARPÍŠKOVÁ (203 Czech Republic) and Jiří DOŠKAŘ (203 Czech Republic, belonging to the institution).
Edition Scientific Reports, London, Nature Publishing Group, 2019, 2045-2322.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10603 Genetics and heredity
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 3.998
RIV identification code RIV/00216224:14310/19:00107363
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1038/s41598-019-41868-w
UT WoS 000462990000017
Keywords in English Kayvirus; Viral genetics; Phage Therapy; Methicillin-Resistant Staphylococcus aureus; Host Range; Genetic Polymorphisms
Tags rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Tibor Botka, Ph.D., učo 177238. Changed: 6/2/2023 13:06.
Abstract
Lytic bacteriophages are valuable therapeutic agents against bacterial infections. There is continual effort to obtain new phages to increase the effectivity of phage preparations against emerging phage-resistant strains. Here we described the genomic diversity of spontaneous host-range mutants of kayvirus 812. Five mutant phages were isolated as rare plaques on phage-resistant Staphylococcus aureus strains. The host range of phage 812-derived mutants was 42% higher than the wild type, determined on a set of 186 methicillin-resistant S. aureus strains representing the globally circulating human and livestock-associated clones. Comparative genomics revealed that single-nucleotide polymorphisms from the parental phage 812 population were fixed in next-step mutants, mostly in genes for tail and baseplate components, and the acquired point mutations led to diverse receptor binding proteins in the phage mutants. Numerous genome changes associated with rearrangements between direct repeat motifs or intron loss were found. Alterations occurred in host-takeover and terminal genomic regions or the endolysin gene of mutants that exhibited the highest lytic activity, which implied various mechanisms of overcoming bacterial resistance. The genomic data revealed that Kayvirus spontaneous mutants are free from undesirable genes and their lytic properties proved their suitability for rapidly updating phage therapeutics.
Links
GA18-13064S, research and development projectName: Analýza interakcí mezi polyvalentním terapeutickým fágem druhu Twort-like a jeho hostitelem Staphylococcus aureus
Investor: Czech Science Foundation
NV16-29916A, research and development projectName: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu
QJ1510216, research and development projectName: Fágová terapie infekcí vyvolaných Staphylococcus aureus v chovech hospodářských zvířat (Acronym: StafyFag)
Investor: Ministry of Agriculture of the CR
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