| BOTKA, Tibor, Roman PANTŮČEK, Ivana MAŠLAŇOVÁ, Martin BENEŠÍK, Petr PETRÁŠ, Vladislava RŮŽIČKOVÁ, Pavla HAVLÍČKOVÁ, Marian VARGA, Helena ŽEMLIČKOVÁ, Ivana KOLÁČKOVÁ, Martina FLORIANOVÁ, Vladislav JAKUBŮ, Renata KARPÍŠKOVÁ and Jiří DOŠKAŘ. Lytic and genomic properties of spontaneous host-range Kayvirus mutants prove their suitability for upgrading phage therapeutics against staphylococci. Scientific Reports. London: Nature Publishing Group, 2019, vol. 9, No 1, p. 5475-5486. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-019-41868-w. |
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@article{1522076,
author = {Botka, Tibor and Pantůček, Roman and Mašlaňová, Ivana and Benešík, Martin and Petráš, Petr and Růžičková, Vladislava and Havlíčková, Pavla and Varga, Marian and Žemličková, Helena and Koláčková, Ivana and Florianová, Martina and Jakubů, Vladislav and Karpíšková, Renata and Doškař, Jiří},
article_location = {London},
article_number = {1},
doi = {http://dx.doi.org/10.1038/s41598-019-41868-w},
keywords = {Kayvirus; Viral genetics; Phage Therapy; Methicillin-Resistant Staphylococcus aureus; Host Range; Genetic Polymorphisms},
language = {eng},
issn = {2045-2322},
journal = {Scientific Reports},
title = {Lytic and genomic properties of spontaneous host-range Kayvirus mutants prove their suitability for upgrading phage therapeutics against staphylococci},
url = {https://www.nature.com/articles/s41598-019-41868-w},
volume = {9},
year = {2019}
}
TY - JOUR
ID - 1522076
AU - Botka, Tibor - Pantůček, Roman - Mašlaňová, Ivana - Benešík, Martin - Petráš, Petr - Růžičková, Vladislava - Havlíčková, Pavla - Varga, Marian - Žemličková, Helena - Koláčková, Ivana - Florianová, Martina - Jakubů, Vladislav - Karpíšková, Renata - Doškař, Jiří
PY - 2019
TI - Lytic and genomic properties of spontaneous host-range Kayvirus mutants prove their suitability for upgrading phage therapeutics against staphylococci
JF - Scientific Reports
VL - 9
IS - 1
SP - 5475
EP - 5475
PB - Nature Publishing Group
SN - 20452322
KW - Kayvirus
KW - Viral genetics
KW - Phage Therapy
KW - Methicillin-Resistant Staphylococcus aureus
KW - Host Range
KW - Genetic Polymorphisms
UR - https://www.nature.com/articles/s41598-019-41868-w
L2 - https://www.nature.com/articles/s41598-019-41868-w
N2 - Lytic bacteriophages are valuable therapeutic agents against bacterial infections. There is continual effort to obtain new phages to increase the effectivity of phage preparations against emerging phage-resistant strains. Here we described the genomic diversity of spontaneous host-range mutants of kayvirus 812. Five mutant phages were isolated as rare plaques on phage-resistant Staphylococcus aureus strains. The host range of phage 812-derived mutants was 42% higher than the wild type, determined on a set of 186 methicillin-resistant S. aureus strains representing the globally circulating human and livestock-associated clones. Comparative genomics revealed that single-nucleotide polymorphisms from the parental phage 812 population were fixed in next-step mutants, mostly in genes for tail and baseplate components, and the acquired point mutations led to diverse receptor binding proteins in the phage mutants. Numerous genome changes associated with rearrangements between direct repeat motifs or intron loss were found. Alterations occurred in host-takeover and terminal genomic regions or the endolysin gene of mutants that exhibited the highest lytic activity, which implied various mechanisms of overcoming bacterial resistance. The genomic data revealed that Kayvirus spontaneous mutants are free from undesirable genes and their lytic properties proved their suitability for rapidly updating phage therapeutics.
ER -
BOTKA, Tibor, Roman PANTŮČEK, Ivana MAŠLAŇOVÁ, Martin BENEŠÍK, Petr PETRÁŠ, Vladislava RŮŽIČKOVÁ, Pavla HAVLÍČKOVÁ, Marian VARGA, Helena ŽEMLIČKOVÁ, Ivana KOLÁČKOVÁ, Martina FLORIANOVÁ, Vladislav JAKUBŮ, Renata KARPÍŠKOVÁ and Jiří DOŠKAŘ. Lytic and genomic properties of spontaneous host-range Kayvirus mutants prove their suitability for upgrading phage therapeutics against staphylococci. \textit{Scientific Reports}. London: Nature Publishing Group, 2019, vol.~9, No~1, p.~5475-5486. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-019-41868-w.
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