TNF alpha and microRNA-15b expression changes in experimental
model of subarachnoid haemorrhage

J 2019

TNF alpha and microRNA-15b expression changes in experimental model of subarachnoid haemorrhage

LIPKOVÁ, Jolana, Zbyněk ŠPLÍCHAL, Michal JURAJDA, Tereza MADARÁSZOVÁ, Anna VAŠKŮ et. al.

Základní údaje

Originální název

TNF alpha and microRNA-15b expression changes in experimental model of subarachnoid haemorrhage

Autoři

LIPKOVÁ, Jolana (203 Česká republika, domácí), Zbyněk ŠPLÍCHAL (203 Česká republika, domácí), Michal JURAJDA (203 Česká republika, garant, domácí), Tereza MADARÁSZOVÁ (203 Česká republika, domácí), Anna VAŠKŮ (203 Česká republika, domácí), Martin SMRČKA (203 Česká republika, domácí) a Kamil ĎURIŠ (203 Česká republika, domácí)

Vydání

Česká a slovenská neurologie a neurochirurgie, Praha, Česká lékařská společnost J.E. Purkyně, 2019, 1210-7859

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30103 Neurosciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 0.377

Kód RIV

RIV/00216224:14110/19:00107364

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.14735/amcsnn201953

UT WoS

000458015800007

Klíčová slova anglicky

subarachnoid haemorrhage; early brain injury; inflammation; apoptosis; microRNA; perforation model

Štítky

14110224, 14110518, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 4. 2019 14:22, Soňa Böhmová

Anotace

V originále

Aim: The aim of the study was to investigate expression changes of pro-inflammatory and proapoptotic cytokine tumor necrosis factor alpha (TNF alpha) and microRNAs (miRNAs) involved in its regulation in early pathophysiological changes after subarachnoid haemorrhage (SAH). Materials and methods: MiRNAs (miR-125b, miR-146a, miR-346, miR-155, miR-15b) and mRNA (TNF alpha) expression were determined by quantitative real-time polymerase chain reaction in brain tissue samples. A total of 88 animals were divided to Sham (control surgery without induction of SAH), Mild SAH, Severe SAH groups in following time-points: 2, 4, 6 and 8 h (n = 7 per group); including 4 animals used as an absolute control. Results: We have found a statistically significant difference in TNF alpha expression between Sham and Severe SAH groups at all the time-points (p < 0.05), between Sham and Mild SAH groups 4 h after induction of SAH (p < 0.05) and between Mild and Severe SAH groups at 2 and 6 h time-points (p < 0.05). Furthermore, a significant difference in miR-15b expression between Sham and Severe SAH groups was observed 8 h after SAH (p < 0.05). All the other microRNAs have not been significantly changed. Conclusions: SAH was associated with an early increase in TNF alpha and miR-15b expression especially in Severe SAH group. Despite complex cross-regulation between cytokines and miRNA, any information about the activation of inflammation/apoptotic mechanisms within a few hours after SAH may improve our knowledge of SAH pathophysiology. Furthermore, it can lead to therapeutic improvement using a combination of both pro-apoptotic markers TNF alpha and miR-15b.

Návaznosti

GP14-23773P, projekt VaV

Název: Regulace zánětlivé odpovědi prostřednictvím mikroRNA na experimaentálním modelu subarachnoidálního krvácení

Investor: Grantová agentura ČR, Regulace zánětlivé odpovědi prostřednictvím mikroRNA na experimaentálním modelu subarachnoidálního krvácení

Citovat

LIPKOVÁ, Jolana, Zbyněk ŠPLÍCHAL, Michal JURAJDA, Tereza MADARÁSZOVÁ, Anna VAŠKŮ, Martin SMRČKA a Kamil ĎURIŠ. TNF alpha and microRNA-15b expression changes in experimental model of subarachnoid haemorrhage. Česká a slovenská neurologie a neurochirurgie. Praha: Česká lékařská společnost J.E. Purkyně, 2019, roč. 82, č. 1, s. 53-59. ISSN 1210-7859. Dostupné z: https://dx.doi.org/10.14735/amcsnn201953.

@article{1522296,
   author = {Lipková, Jolana and Šplíchal, Zbyněk and Jurajda, Michal and Madarászová, Tereza and Vašků, Anna and Smrčka, Martin and Ďuriš, Kamil},
   article_location = {Praha},
   article_number = {1},
   doi = {http://dx.doi.org/10.14735/amcsnn201953},
   keywords = {subarachnoid haemorrhage; early brain injury; inflammation; apoptosis; microRNA; perforation model},
   language = {eng},
   issn = {1210-7859},
   journal = {Česká a slovenská neurologie a neurochirurgie},
   title = {TNF alpha and microRNA-15b expression changes in experimental model of subarachnoid haemorrhage},
   url = {http://dx.doi.org/10.14735/amcsnn201953},
   volume = {82},
   year = {2019}
}

TY  - JOUR
ID  - 1522296
AU  - Lipková, Jolana - Šplíchal, Zbyněk - Jurajda, Michal - Madarászová, Tereza - Vašků, Anna - Smrčka, Martin - Ďuriš, Kamil
PY  - 2019
TI  - TNF alpha and microRNA-15b expression changes in experimental model of subarachnoid haemorrhage
JF  - Česká a slovenská neurologie a neurochirurgie
VL  - 82
IS  - 1
SP  - 53-59
EP  - 53-59
PB  - Česká lékařská společnost J.E. Purkyně
SN  - 12107859
KW  - subarachnoid haemorrhage
KW  - early brain injury
KW  - inflammation
KW  - apoptosis
KW  - microRNA
KW  - perforation model
UR  - http://dx.doi.org/10.14735/amcsnn201953
L2  - http://dx.doi.org/10.14735/amcsnn201953
N2  - Aim: The aim of the study was to investigate expression changes of pro-inflammatory and proapoptotic cytokine tumor necrosis factor alpha (TNF alpha) and microRNAs (miRNAs) involved in its regulation in early pathophysiological changes after subarachnoid haemorrhage (SAH). Materials and methods: MiRNAs (miR-125b, miR-146a, miR-346, miR-155, miR-15b) and mRNA (TNF alpha) expression were determined by quantitative real-time polymerase chain reaction in brain tissue samples. A total of 88 animals were divided to Sham (control surgery without induction of SAH), Mild SAH, Severe SAH groups in following time-points: 2, 4, 6 and 8 h (n = 7 per group); including 4 animals used as an absolute control. Results: We have found a statistically significant difference in TNF alpha expression between Sham and Severe SAH groups at all the time-points (p < 0.05), between Sham and Mild SAH groups 4 h after induction of SAH (p < 0.05) and between Mild and Severe SAH groups at 2 and 6 h time-points (p < 0.05). Furthermore, a significant difference in miR-15b expression between Sham and Severe SAH groups was observed 8 h after SAH (p < 0.05). All the other microRNAs have not been significantly changed. Conclusions: SAH was associated with an early increase in TNF alpha and miR-15b expression especially in Severe SAH group. Despite complex cross-regulation between cytokines and miRNA, any information about the activation of inflammation/apoptotic mechanisms within a few hours after SAH may improve our knowledge of SAH pathophysiology. Furthermore, it can lead to therapeutic improvement using a combination of both pro-apoptotic markers TNF alpha and miR-15b.
ER  -

LIPKOVÁ, Jolana, Zbyněk ŠPLÍCHAL, Michal JURAJDA, Tereza MADARÁSZOVÁ, Anna VAŠKŮ, Martin SMRČKA a Kamil ĎURIŠ. TNF alpha and microRNA-15b expression changes in experimental model of subarachnoid haemorrhage. \textit{Česká a slovenská neurologie a neurochirurgie}. Praha: Česká lékařská společnost J.E. Purkyně, 2019, roč.~82, č.~1, s.~53-59. ISSN~1210-7859. Dostupné z: https://dx.doi.org/10.14735/amcsnn201953.

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