Enterovirus particles expel capsid pentamers to enable genome
release

J 2019

Enterovirus particles expel capsid pentamers to enable genome release

BUCHTA, David, Tibor FÜZIK, Dominik HREBÍK, Yevgen LEVDANSKY, Lukáš SUKENÍK et. al.

Basic information

Original name

Enterovirus particles expel capsid pentamers to enable genome release

Authors

BUCHTA, David (203 Czech Republic, belonging to the institution), Tibor FÜZIK (703 Slovakia, belonging to the institution), Dominik HREBÍK (703 Slovakia, belonging to the institution), Yevgen LEVDANSKY (804 Ukraine, belonging to the institution), Lukáš SUKENÍK (203 Czech Republic, belonging to the institution), Liya MUKHAMEDOVA (643 Russian Federation, belonging to the institution), Jana MORAVCOVÁ (203 Czech Republic, belonging to the institution), Robert VÁCHA (203 Czech Republic, belonging to the institution) and Pavel PLEVKA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Nature Communications, London, Nature Publishing Group, 2019, 2041-1723

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10607 Virology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 12.121

RIV identification code

RIV/00216224:14740/19:00107387

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1038/s41467-019-09132-x

UT WoS

000460631100023

Keywords in English

MOLECULAR-DYNAMICS; CONFORMATIONAL-CHANGES; FORCE-FIELD; CELL ENTRY; POLIOVIRUS; VIRUS; RNA; MODEL; TRANSITIONS; RECOGNITION

Abstract

V originále

Viruses from the genus Enterovirus are important human pathogens. Receptor binding or exposure to acidic pH in endosomes converts enterovirus particles to an activated state that is required for genome release. However, the mechanism of enterovirus uncoating is not well understood. Here, we use cryo-electron microscopy to visualize virions of human echovirus 18 in the process of genome release. We discover that the exit of the RNA from the particle of echovirus 18 results in a loss of one, two, or three adjacent capsid-protein pentamers. The opening in the capsid, which is more than 120 A in diameter, enables the release of the genome without the need to unwind its putative double-stranded RNA segments. We also detect capsids lacking pentamers during genome release from echovirus 30. Thus, our findings uncover a mechanism of enterovirus genome release that could become target for antiviral drugs.

Links

GX19-25982X, research and development project

Name: Analýza replikace enterovirů s využitím elektronové mikroskopie

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

3041, interní kód MU

Name: Structural studies of human and animal pathogens from the order Picornavirales

Investor: EMBO (European Molecular Biology Organization)

335855, interní kód MU

Name: Structural studies of human picornaviruses directed towards development of anti-viral compounds (Acronym: PicoDrugs)

Investor: European Union, Ideas

90043, large research infrastructures

Name: CIISB

Citovat

BUCHTA, David, Tibor FÜZIK, Dominik HREBÍK, Yevgen LEVDANSKY, Lukáš SUKENÍK, Liya MUKHAMEDOVA, Jana MORAVCOVÁ, Robert VÁCHA and Pavel PLEVKA. Enterovirus particles expel capsid pentamers to enable genome release. Nature Communications. London: Nature Publishing Group, 2019, vol. 10, MAR, p. 1-9. ISSN 2041-1723. Available from: https://dx.doi.org/10.1038/s41467-019-09132-x.

@article{1528796,
   author = {Buchta, David and Füzik, Tibor and Hrebík, Dominik and Levdansky, Yevgen and Sukeník, Lukáš and Mukhamedova, Liya and Moravcová, Jana and Vácha, Robert and Plevka, Pavel},
   article_location = {London},
   article_number = {MAR},
   doi = {http://dx.doi.org/10.1038/s41467-019-09132-x},
   keywords = {MOLECULAR-DYNAMICS; CONFORMATIONAL-CHANGES; FORCE-FIELD; CELL ENTRY; POLIOVIRUS; VIRUS; RNA; MODEL; TRANSITIONS; RECOGNITION},
   language = {eng},
   issn = {2041-1723},
   journal = {Nature Communications},
   title = {Enterovirus particles expel capsid pentamers to enable genome release},
   url = {https://www.nature.com/articles/s41467-019-09132-x.pdf},
   volume = {10},
   year = {2019}
}

TY  - JOUR
ID  - 1528796
AU  - Buchta, David - Füzik, Tibor - Hrebík, Dominik - Levdansky, Yevgen - Sukeník, Lukáš - Mukhamedova, Liya - Moravcová, Jana - Vácha, Robert - Plevka, Pavel
PY  - 2019
TI  - Enterovirus particles expel capsid pentamers to enable genome release
JF  - Nature Communications
VL  - 10
IS  - MAR
SP  - 1-9
EP  - 1-9
PB  - Nature Publishing Group
SN  - 20411723
KW  - MOLECULAR-DYNAMICS
KW  - CONFORMATIONAL-CHANGES
KW  - FORCE-FIELD
KW  - CELL ENTRY
KW  - POLIOVIRUS
KW  - VIRUS
KW  - RNA
KW  - MODEL
KW  - TRANSITIONS
KW  - RECOGNITION
UR  - https://www.nature.com/articles/s41467-019-09132-x.pdf
L2  - https://www.nature.com/articles/s41467-019-09132-x.pdf
N2  - Viruses from the genus Enterovirus are important human pathogens. Receptor binding or exposure to acidic pH in endosomes converts enterovirus particles to an activated state that is required for genome release. However, the mechanism of enterovirus uncoating is not well understood. Here, we use cryo-electron microscopy to visualize virions of human echovirus 18 in the process of genome release. We discover that the exit of the RNA from the particle of echovirus 18 results in a loss of one, two, or three adjacent capsid-protein pentamers. The opening in the capsid, which is more than 120 A in diameter, enables the release of the genome without the need to unwind its putative double-stranded RNA segments. We also detect capsids lacking pentamers during genome release from echovirus 30. Thus, our findings uncover a mechanism of enterovirus genome release that could become target for antiviral drugs.
ER  -

BUCHTA, David, Tibor FÜZIK, Dominik HREBÍK, Yevgen LEVDANSKY, Lukáš SUKENÍK, Liya MUKHAMEDOVA, Jana MORAVCOVÁ, Robert VÁCHA and Pavel PLEVKA. Enterovirus particles expel capsid pentamers to enable genome release. \textit{Nature Communications}. London: Nature Publishing Group, 2019, vol.~10, MAR, p.~1-9. ISSN~2041-1723. Available from: https://dx.doi.org/10.1038/s41467-019-09132-x.

Detailed Information on Publication Record